Yin nazarin leukocytes cikin jini gabaɗaya shine gwajin yau da kullun a cikin dakin gwaje-gwaje na asibiti ko bankin jini.Mahimmanci da iyawar leukocytes sune maƙasudin mahimmanci a matsayin ingancin kula da ajiyar jini.Baya ga leukocyte, duka jini yana ɗauke da adadi mai yawa na platelets, jajayen ƙwayoyin jini, ko tarkace ta salula, waɗanda ke sa ba zai yiwu a iya tantance cikakken jini ba kai tsaye a ƙarƙashin na'urar hangen nesa ko na'urar tantanin halitta mai haske.Hanyoyi na al'ada don ƙidaya fararen jinin jini sun haɗa da tsarin RBC lysis, wanda yake cin lokaci.
AOPI Dual-fluoresces ƙidayar ita ce nau'in tantancewar da ake amfani da ita don gano tattarawar tantanin halitta da yuwuwa.Maganin shine hadewar acridine orange (koren-fluorescent nucleic acid tabo) da kuma propidium iodide (tabon nucleic acid ja-fluorescent).Propidium iodide (PI) wani launi ne na keɓan membrane wanda kawai ke shiga sel tare da membranes masu rikitarwa, yayin da acridine orange yana iya shiga duk sel a cikin yawan jama'a.Lokacin da rinannun biyu ke kasancewa a cikin tsakiya, propidium iodide yana haifar da raguwar acridine orange fluorescence ta hanyar canja wurin makamashi mai haske (FRET).Sakamakon haka, ƙwayoyin sel waɗanda ke da sel ɗin da ba su da kyau suna lalata kore mai kyalli kuma ana kirga su azaman masu rai, yayin da ƙwayoyin sel waɗanda ke da alaƙa da membranes kawai suna lalata ja kuma ana ƙidaya su a matsayin matattu yayin amfani da tsarin Countstar® Rigel.
Countstar Rigel shine ingantaccen bayani don ƙididdige ƙididdige yawan adadin ƙwayoyin sel, yana ba da damar bincikar fararen-jini cikin sauri cikin jini gaba ɗaya.
Tsarin Gwaji:
1.Dauki 20 µl na samfurin jini kuma tsarma samfurin a cikin 180 µl na PBS.
2. Ƙara 12µl AO / PI bayani a cikin samfurin 12µl, a hankali gauraye da pipette;
3. Zana 20µl cakuda cikin ɗakin zamewa;
4.Ba da izinin sel su zauna a cikin ɗakin don kimanin minti 1;
5.Insect da zamewar cikin Countstar FL kayan aiki;
6.Zaɓi gwajin "AO/PI Viability", sannan Shigar da Sample ID don wannan samfurin.
7.Select Dilution rabo, Cell Type, danna 'Run' don fara gwajin.
Tsanaki: AO da PI ne mai yuwuwar cutar kansa.Ana ba da shawarar cewa ma'aikacin ya sa kayan kariya na sirri (PPE) don guje wa haɗuwa da fata da idanu kai tsaye.
Sakamako:
1. Hoton fili mai haske na duka jini
A cikin hoton fili mai haske na duka jini, WBC ba a iya gani a cikin jajayen tantanin halitta.(Hoto na 1)
Hoto 1 Hoton fili mai haske na cikakken jini.
2. Fluorescence Hoton dukkan jini
Rini na AO da PI duka suna tabo DNA a cikin tsakiya na sel.Saboda haka, Platelets, jajayen ƙwayoyin jini, ko tarkace ta salula ba su da ikon yin tasiri ga tattarawar leukocytes da sakamako mai yiwuwa.Leukocytes masu rai (Green) da matattu leukocytes (Red) ana iya gani cikin sauƙi a cikin hotunan kyalli.(Hoto na 2)
Hoto na 2 Hotunan Fluorescence na dukkan jini.(A).Hoton tashar AO;(B) Hoton PI Channel;(C) Haɗa hotunan AO da PI Channel.
3. Tattaunawa da iyawar leukocytes
Software na Countstar FL ta atomatik yana ƙididdige sel na sassan ɗaki uku kuma yana ƙididdige matsakaicin ƙimar jimlar adadin tantanin halitta WBC (1202), maida hankali (1.83 x 106 sel/ml), da % yiwuwa (82.04%).Ana iya fitar da dukkan hotunan jini da bayanan cikin sauƙi azaman PDF, Hoto ko Excel don ƙarin bincike ko adana bayanai.
Hoto 3 Hoton Hoton Countstar Rigel Software
