Dual-fluorescence Viability (AO/PI), Acridine orange (AO) da propidium iodide (PI) sune tabon nucleic da rini mai ɗaure acid.AO na iya shiga cikin membrane na matattu da raye-rayen sel kuma yana lalata tsakiya, yana haifar da haske mai kore.Sabanin haka, PI kawai na iya ratsa ɓangarorin ɓarkewar ƙwayoyin matattu, suna haifar da haske mai haske.Fasaha na tushen hoto na Countstar Rigel ya keɓance gutsuttsuran tantanin halitta, tarkace, da ɓangarorin kayan tarihi da kuma abubuwan da ba su da girma kamar platelets, suna ba da kyakkyawan sakamako.A ƙarshe, ana iya amfani da tsarin Countstar Rigel don kowane mataki na tsarin kera tantanin halitta.

T/NK Cell-Mediated Cytotoxicity, A cikin kwanan nan FDA-an yarda da CAR-T cell far, kwayoyin halitta T-lymphocytes ɗaure musamman zuwa ga ciwon daji Kwayoyin (T) da kuma kashe su.Masu nazarin Countstar Rigel sun sami damar yin nazarin wannan cikakken tsari na T/NK Cell-Mediated Cytotoxicity.

Ana yin nazarin cytotoxicity ta hanyar sanya maƙasudin ƙwayoyin cutar kansa da CFSE ko canza su da GFP.Ana iya amfani da Hoechst 33342 don tabo dukkan sel (duka ƙwayoyin T da ƙwayoyin ƙari).A madadin, ƙwayoyin tumor da aka yi niyya za a iya lalata su da CFSE.Ana amfani da Propidium iodide (PI) don tabo matattun ƙwayoyin cuta (duka ƙwayoyin T da ƙwayoyin ƙari).Ana iya samun wariya tsakanin sel daban-daban ta amfani da wannan dabarar tabo.

Ingantaccen Canjin GFP, A cikin kwayoyin halittar kwayoyin halitta, nau'ikan nau'ikan nau'ikan halittu, da ilmin halitta, ana yawan amfani da kwayar GFP azaman mai ba da rahoto don nazarin magana.A halin yanzu, masana kimiyya galibi suna amfani da microscopes masu kyalli ko na'urorin sitometer masu gudana don tantance ingancin canjin ƙwayoyin dabbobi masu shayarwa.Amma sarrafa hadaddun fasaha na ci-gaba na cytometer kwarara yana buƙatar ƙwararren ƙwararren mai aiki.Countstar Rigel yana bawa masu amfani damar yin ƙayyadaddun ƙayyadaddun ƙayyadaddun canzawa cikin sauƙi da daidai ba tare da aiki da ƙimar kulawa da ke da alaƙa da sitometry kwarara na gargajiya ba.

Cell Apoptosis, Ana iya lura da ci gaban apoptosis cell ta amfani da FITC conjugated Annexin-V a hade tare da 7-ADD.Ragowar Phosphatidylserine (PS) suna yawanci a gefen ciki na membrane plasma na sel lafiya.A lokacin apoptosis na farko, mutuncin membrane yana ɓacewa kuma PS za a canza shi zuwa waje na tantanin halitta.Annexin V yana da alaƙa mai ƙarfi ga PS kuma don haka shine madaidaicin alama don ƙwayoyin apoptotic na farko.

Zagayowar salula, Yayin rarraba tantanin halitta, sel sun ƙunshi ƙarin adadin DNA.PI wanda aka yiwa lakabi da shi, haɓakar ƙarfin haske yana daidai da tarin DNA kai tsaye.Bambance-bambance a cikin ƙarfin hasken wuta na sel guda ɗaya sune masu nuna alamar ainihin matsayi na sake zagayowar tantanin halitta MCF 7 Kwayoyin da aka bi da su tare da 4μM na Nocodazole don kama waɗannan kwayoyin halitta a matakai daban-daban na sake zagayowar tantanin halitta.Hotunan fili mai haske da aka samu yayin wannan yanayin gwaji ya ba mu damar gano kowane tantanin halitta guda.Tashar fluorescence ta PI na Countstar Rigel tana gano siginar DNA na sel guda ko da a cikin tari.Ana iya yin cikakken bincike game da ƙarfin hasken wuta ta amfani da FCS.

CD Marker Phenotyping, Samfuran Countstar Rigel suna ba da hanya mai sauri, mafi sauƙi kuma mafi mahimmanci ga, ƙirar ƙwayoyin ƙwayoyin cuta masu inganci.Tare da manyan hotuna masu ƙarfi da ƙarfin bincike na bayanai masu ƙarfi, Countstar Rigel yana bawa masu amfani damar cimma ingantaccen sakamako mai inganci ba tare da buƙatar manyan saitunan sarrafawa da gyare-gyaren ramuwa mai haske ba.

Bambance-bambancen kwayar cutar Cytokine Induced Killer (CIK) yana nuna ingantaccen ingancin aikin mai nazarin Countstar Rigel a kwatanta kai tsaye zuwa manyan sitometer masu gudana.PBMCs na linzamin kwamfuta a cikin al'ada an lalata su tare da CD3-FITC, CD4-PE, CD8-PE, da CD56-PE, kuma Interleukin (IL) 6 ya haifar da shi. Sa'an nan kuma an bincika tare da Countstar® Rigel da Flow Cytometry.A cikin wannan gwajin, CD3-CD4, CD3-CD8, da CD3-CD56 an raba su zuwa rukuni uku, don sanin adadin ƙananan ƙwayoyin sel daban-daban.

Gano Lalacewar Kwayoyin Ta hanyar Immunofluorescence, ƙwayoyin rigakafi na Monoclonal da ke samar da layin tantanin halitta za su rasa wasu kyawawan clones yayin yaduwar kwayar halitta da wucewa saboda lalacewa ko maye gurbi.Babban hasara zai haifar da tasiri sosai ga yawan aiki na tsarin masana'antu.Saka idanu na lalacewa yana taka muhimmiyar rawa a cikin sarrafa tsari don canza yawan amfanin kwayoyin zuwa mafi kyau.

Yawancin ƙwayoyin rigakafi da aka ƙera a cikin masana'antar BioPharma ana iya gano su ta lakabin immunofluorescence kuma an bincika su da ƙima ta jerin Countstar Rigel.Hotunan tashoshi mai haske da kyalli da ke ƙasa suna nuna a fili waɗancan clones waɗanda suka rasa sifa don samar da ƙwayoyin rigakafin da ake so.Cikakken cikakken bincike tare da software na DeNovo FCS Express Hoton ya tabbatar, cewa 86.35 % na duk sel suna bayyana immunoglobulins, kawai 3.34% ba su da kyau.

Trypan (ya zama babban B a cikin Blue) Ƙididdiga ta salula, Trypan blue tabo har yanzu ana amfani da shi a yawancin ɗakunan binciken al'adun tantanin halitta.

Ana iya shigar da Tripan Blue Viability da Cell Density BioApp akan duk samfuran Countstar Rigel.Algorithms na tantance hoton mu masu kariya suna tantance sigogi sama da 20 don rarraba kowane abu guda daya da aka gano.

Ma'ajiyar Layin Cell QC, A cikin ma'ajin tantanin halitta, ingantaccen ra'ayi na gudanarwa yana tabbatar da aminci, ingantaccen kulawa na duk samfuran salula.Wannan yana ba da tabbacin ingantaccen ingancin cell cryopreserved, cryo-tsare don gwaje-gwaje, haɓaka tsari, da samarwa.

Countstar Rigel yana samun hotuna masu girman gaske, yana nazarin halaye daban-daban na halittar abubuwa na salon salula kamar diamita, siffa, da halin tarawa.Hotuna na matakai daban-daban na tsari za a iya sauƙin kwatanta da juna.Don haka ana iya gano bambance-bambancen siffa da tarawa cikin sauƙi, ta hanyar guje wa ma'aunin ɗan adam.Kuma bayanan Countstar Rigel yana da ingantaccen tsarin gudanarwa don adanawa da dawo da hotuna da bayanai.