Countstar Mira FL

Siffofin samfur

Ƙirƙirar Fasahar Haɓakawa Na gani

Fasahar zuƙowa ta musamman tana ba masu amfani damar tantance sel a cikin kewayon diamita

Lokacin amfani da samfurin BioApp mai haske a cikin Countstar Mira, sabuwar fasahar Zuƙowa tana bawa mai aiki damar gano ainihin abubuwan salula a cikin kewayon diamita daga 1.0µm zuwa 180.0µm.Hotunan da aka samo suna nuna ko da cikakkun bayanai na sel guda ɗaya.Wannan yana faɗaɗa kewayon aikace-aikace har zuwa abubuwan salula, waɗanda ba za a iya tantance su daidai a baya ba.

Algorithms na Binciken Hoto na Ci gaba na AI

Countstar Mira FL yana amfani da fa'idodin Hankali na Artifical don haɓaka algorithms na koyan kai.Suna iya ganowa da kuma nazarin halaye masu yawa na sel.Haɗuwa da sigogin sifofin tantanin halitta yana ba da damar yin nazari sosai da ƙididdiga game da matsayi na sake zagayowar tantanin halitta da / ko isar da bayanai game da alaƙa tsakanin canji a cikin ilimin halittar jiki, samuwar gungu na tantanin halitta (aggregates, ƙananan spheroids) da yanayin da ke da tasiri.

Lakabin sakamako na Siffar Mesenchymal Stem Cells (MSC; 5x mangification) a cikin al'adun da ke yaduwa.

• Koren da'irori suna alamar sel masu rai
• Jajayen da'irar alama ce ta matattu
• Farin da'irori sun taru

Layin tantanin halitta RAW264.7 shine ƙarami kuma cikin sauƙi.Algorithm na Countstar AI na iya gano sel a cikin gungu da ƙidaya

• Koren da'irori suna alamar sel masu rai
• Jajayen da'irar alama ce ta matattu
• Farin da'irori sun taru

Girman da ba daidai ba na ƙwayoyin mahaifa na zebrafish (girma 6.6X

• Koren da'irori suna alamar sel masu rai
• Jajayen da'irar alama ce ta matattu
• Farin da'irori sun taru

Ƙirƙirar Ƙirƙirar Ƙwararrun Mai Amfani (GUI).

GUI da aka tsara bayyananne yana ba da izinin aiwatar da gwaji mai inganci da dadi

• Babban ɗakin karatu tare da nau'ikan tantanin halitta da aka riga aka saita da BioApps (ka'idojin samfuri).Dannawa ɗaya kawai akan BioApp, kuma gwajin na iya farawa.
• GUI mai sauƙin amfani yana sauƙaƙa sauyawa tsakanin zaɓuɓɓukan menu daban-daban kuma yana ba da garantin ƙwarewar gwaji mai daɗi
• Share tsarin menu wanda aka tsara yana goyan bayan mai amfani a cikin aikin gwajin yau da kullun

Zaɓi BioApp, shigar da Samfurin ID, sannan fara aikin tantancewa

128 GB na iyawar ma'ajiyar bayanai ta tsaka-tsaki, ya isa don adana kusan.Sakamakon bincike na 50,000 a cikin Countstar (R) Mira.Don samun dama cikin sauri, ana iya zaɓar bayanan da ake so ta zaɓuɓɓukan bincike daban-daban.

Siffa mai fa'ida don adana lokaci, ita ce kalkuleta mai narkewa mai iya dawo da ita.Zai isar da ainihin kundin diluent da samfurin tantanin halitta na asali, da zarar an shigar da ƙaddamarwar ƙarshe na sel da ƙarar manufa.Wannan yana ba da damar wucewar sel zuwa al'adun su cikin kwanciyar hankali.

Fasalolin aikace-aikacen da yawa

Siffofin bincike na Countstar Mira suna tallafawa mai amfani don fahimtar canje-canje masu ƙarfi a cikin al'adar tantanin halitta kuma yana taimakawa haɓaka yanayin haɓakarsu.

Ci gaba, software na tushen AI na gano hoto na Countstar Mira yana da ikon sadar da sigogi da yawa.Bayan daidaitattun sakamakon tattarawar tantanin halitta da matsayi mai yiwuwa, rarraba girman tantanin halitta, yuwuwar samuwar gungu na tantanin halitta, ƙarancin haske na kowane tantanin halitta guda ɗaya, nau'in lanƙwan girma, da yanayin yanayin halittarsu na waje sune mahimman sigogi don tantance ainihin ainihin. yanayin al'adun tantanin halitta.Hotunan da aka samar ta atomatik na masu lanƙwasa girma, rarraba diamita da ƙididdiga masu ƙarfi na haske, nazarin tantanin halitta guda ɗaya a cikin tarawa da ƙayyadaddun ma'aunin ƙayyadaddun tantanin halitta yana sauƙaƙe mai amfani don ƙarin fahimtar matakai masu ƙarfi a cikin al'adun tantanin halitta da aka bincika daga farkon zuwa ƙarewar tsari.

Histogram

Dangantakar Fluorescence Intensity (RFI) rarraba histogram

Histogram na rarraba diamita

Lanƙwan girma

Gwajin Hoto(s) da Sakamako

Zane mai lanƙwasa girma

Aikace-aikacen samfur

AO/PI dual fluorescence density na cell da kuma iya aiki

Hanya mai dual-fluorescence AO/PI tana dogara ne akan ka'ida, cewa duka rinayen, Acridine Orange (AO) da Propidium Iodide (PI), suna shiga tsakani tsakanin acid nucleic na chromosome a cikin tsakiya ta tantanin halitta.Duk da yake AO yana iya shiga cikin ƙananan membranes na tsakiya a kowane lokaci kuma ya lalata DNA, PI kawai zai iya wuce ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta.AO da aka tara a cikin kwayar tantanin halitta yana fitar da haske kore a iyakar 525nm, idan an yi farin ciki a 480nm, PI yana aika haske mai ja tare da girmansa a 615nm, lokacin da aka yi farin ciki a 525nm.Tasirin FRET (Foerster Resonance Energy Canja wurin) yana ba da garanti, cewa siginar da aka fitar na AO a 525nm ana ɗauka a gaban rini na PI don guje wa fitowar haske biyu da zube.Wannan haɗin rini na musamman na AO/PI yana ba da damar tace musamman tsakiya mai ɗauke da sel a gaban acaryotes kamar erythrocytes.

Bayanan Countstar Mira FL sun nuna kyakkyawan layi don haɓakar sel HEK293

GFP/RFP nazarin ingancin canjin canji

Ingantacciyar hanyar canja wuri muhimmin ma'auni ne a cikin haɓaka layin salula da haɓakawa, a cikin daidaitawar ƙwayoyin cuta, da kuma sa ido kan yawan amfanin samfur a cikin hanyoyin Biopharma.Ya zama gwajin da aka kafa akai-akai don tantance ainihin abin da ke cikin furotin da aka yi niyya a cikin tantanin halitta.A cikin hanyoyin magance kwayoyin halitta daban-daban, kayan aiki ne da ba makawa don sarrafa ingancin canjin yanayin da ake so.

Countstar Mira ba wai kawai yana bayar da madaidaitan sakamako ba, idan aka kwatanta da cytometry mai gudana, bugu da žari mai nazarin yana ba da hotuna a matsayin shaidar shaida.Bayan wannan, yana da mahimmanci sauƙaƙa da saurin bincike don daidaita ci gaban ci gaba da tsarin samarwa.

Jerin hotuna, wanda Countstar (R) Mira ya samu, yana nuna haɓaka haɓakar matakan canzawa (daga hagu zuwa dama) na ƙwayoyin halitta da aka gyara (layin tantanin halitta HEK 293; bayyana GFP a cikin ƙira daban-daban)

Sakamako na ma'auni, wanda aka kashe tare da B/C CytoFLEX, yana mai tabbatar da ingancin canjin GFP na sel HEK 293 da aka gyara, an bincika a cikin Countstar Mira

Ƙididdiga da aka kafa Trypan Blue bincike mai yiwuwa

Ƙididdigar nuna wariya ta Trypan Blue har yanzu tana ɗaya daga cikin hanyoyin da aka fi amfani da su kuma amintacce don tantance adadin matattun ƙwayoyin sel a cikin al'adar tantanin halitta.Kwayoyin da za a iya amfani da su tare da ingantaccen tsarin membrane na tantanin halitta za su kori Trypan Blue daga ratsa cikin membrane.A cikin yanayin, membrane na tantanin halitta ya sami yoyo saboda ci gaban mutuwar tantanin halitta, Trypan Blue na iya wuce shingen membrane, ya taru a cikin plasma ta tantanin halitta kuma ya lalata tantanin halitta blue.Ana iya amfani da wannan bambance-bambancen gani don bambance sel marasa rai daga matattu ta hanyar gano algorithms na hoto na Countstar Mira FL.

• Hotunan uku, Trypan Blue layukan tantanin halitta, da aka samu a cikin Countstar (R) Mira FL a cikin yanayin filin haske.

• Sakamako na dilution gradient na jerin HEK 293