Protocol na gwaji
Ana ƙididdige cytotoxicity% ta lissafin da ke ƙasa.
Cytotoxicity% = (ƙididdigar sarrafawa - ƙididdige yawan jiyya) / Ƙididdigar sarrafawa × 100
Ta hanyar sanya maƙasudin ƙwayoyin ƙwayar cuta da marasa guba, calcein AM marasa radiyo ko canzawa tare da GFP, zamu iya saka idanu akan kashe ƙwayoyin tumor ta ƙwayoyin CAR-T.Yayin da kwayoyin cutar kansa masu rai za a yi wa lakabi da koren calcein AM ko GFP, matattun kwayoyin halitta ba za su iya rike koren rini ba.Ana amfani da Hoechst 33342 don tabo dukkan sel (duka ƙwayoyin T da ƙwayoyin ƙari), a madadin, ƙwayoyin tumor da aka yi niyya za a iya lalata su da membrane daure calcein AM, ana amfani da PI don tabo matattun sel (duka ƙwayoyin T da ƙwayoyin ƙari).Wannan dabarar tabo tana ba da damar bambance sel daban-daban.
E: T Ratio dogara Cytotoxicity na K562
Misali Hoechst 33342, CFSE, PI hotuna masu kyalli su ne K562 sel masu manufa a t = 3 hours
Hotunan da aka samo asali sun nuna karuwa a cikin Hoechst + CFSE + PI + Target cell kamar yadda E: T rabo ya karu.
