Iiseli ze-Mammalian zisetyenziswa kakhulu kwi-Biopharmaceuticals, ezifana ne-antibodies, isitofu sokugonya, i-peptides kunye ne-metabolites yesibini ziveliswa nge-bioprocessing kunye neeseli ze-mammalian.Ngexesha lenkqubo yonke ukusuka kwi-antibody R&D ukuya kwimveliso, maninzi amanyathelo afunekayo ukwenza uvavanyo olusekwe kwiseli ukuvavanya inkqubo okanye ulawulo lomgangatho.Okufana noxinzelelo lweeseli ezipheleleyo kunye nokusebenza kuya kuchaza imeko yenkcubeko yeseli.Kunye nokudluliselwa kweeseli, ukunxulumana kwe-antibody kumisela kwinqanaba leseli.Izixhobo ze-Countstar ziyi-cytometry esekelwe kumfanekiso, inokunceda ukubeka iliso ukusuka kwi-R & D ukuya kwiinkqubo zokuvelisa kunye nokuqinisekisa ukuveliswa kunye nokuhambelana.
Ukubalwa kweeSeli kunye nokuSebenza ngoMgaqo we-Trypan Blue Staining
Ukubeka iliso kunye nokuhlalutya inkcubeko yeseli kunye nezisombululo zanamhlanje.Ukubeka iliso okuthembekileyo nokusebenzayo kubalulekile ekwandiseni isivuno kunye nomgangatho wemveliso kuba notshintsho oluncinci kwiparamitha ye-bioprocess inokuphembelela ukusebenza kwenkcubeko yeeseli zakho.Ukubalwa kweeseli kunye nokusebenza zezona parameters zibaluleke kakhulu, i-Countstar Altair ibonelela ngobuchule obugqithisileyo kwaye ithobela ngokupheleleyo isisombululo se-cGMP kwezi.
I-Countstar Altair iyilwe ngokusekwe kumgaqo wokukhutshelwa we-Trypan Blue wakudala, odibanisa ibhentshi yokujonga "yokulungisa" ye-optical imaging, eyona teknoloji iphambili yokuqondwa kweeseli, kunye ne-algorithms yesoftware.Nika amandla ukufumana ulwazi loxinaniso lweseli, ukusebenza, umyinge wokudityaniswa, ukungqukuva, kunye nokusasazwa kwedayamitha ngokubaleka okunye.
Ukusebenza kunye neGFP Transfection Detemination in Cells
Ngexesha le-bioprocess, i-GFP isoloko isetyenziselwa ukuxubha kunye neprotheni edibeneyo njengesalathisi.Ukumisela i-fluorescent ye-GFP ingabonakalisa inkcazo yeprotheyini ekujoliswe kuyo.I-Countstar Rigel ibonelela ngovavanyo olukhawulezayo nolulula lovavanyo losulelo lwe-GFP kunye nokusebenza.Iiseli zadyojwa ngePropidium iodide (PI) kunye neHoechst 33342 ukuchaza inani leeseli ezifileyo kunye neeseli ezipheleleyo.I-Countstar Rigel ibonelela ngendlela ekhawulezayo, yobungakanani bokuvavanya ukusebenza kakuhle kokubonakaliswa kwe-GFP kunye nokusebenza ngexesha elinye.
Iiseli zibekwe kusetyenziswa i-Hoechst 33342 (ebhlowu) kwaye ipesenti ye-GFP ebonakalisa iiseli (eluhlaza) inokumiselwa ngokulula.Iseli engasebenziyo ingcoliswe yipropidium iodide (PI; ebomvu).
Ukuhambelana nokufunyanwa kwe-antibody kwi-Countstar Rigel
I-antibody ixutywe kwiindawo ezahlukeneyo, emva koko ifakwe kwiiseli.Iziphumo zifunyenwe kwi-Countstar Rigel (zombini umfanekiso kunye neziphumo zobungakanani)
I-Countstar yi-GMP-ilungele i-21 CFR iSigaba se-11
Izixhobo ze-Countstar zihambelana ngokupheleleyo ne-21 CFR kunye neCandelo le-11, iinkonzo ze-IQ / OQ / PQ ziqinisekisa ukulawulwa komsebenzi ohambelanayo.Izixhobo zokubala zilungele ukuphunyezwa kwi-GMP kunye ne-21 CFR inxalenye ye-11 iilabhoratri ezithobelayo.Ulawulo lomsebenzisi kunye neendlela zophicotho zivumela amaxwebhu afanelekileyo okusetyenziswa kunye neengxelo ezisemgangathweni zePDF.
Amaxwebhu e-IQ/OQ kunye neenxalenye zokuqinisekisa
