Mammalian hlwb yog dav siv nyob rau hauv Biopharmaceuticals, xws li tshuaj tiv thaiv, tshuaj tiv thaiv, peptides thiab lwm yam metabolites yog tsim los ntawm bioprocessing nrog mammalian hlwb.Thaum lub sij hawm tag nrho cov txheej txheem los ntawm antibody R & D mus rau ntau lawm, muaj ntau ntau cov kauj ruam yuav tsum tau mus nqa tawm ntawm tes raws li kev soj ntsuam los soj ntsuam cov txheej txheem los yog zoo tswj.Xws li tag nrho cell concentration thiab viability yuav txhais cov xwm txheej ntawm cell kab lis kev cai.Raws li kev hloov pauv ntawm tes, cov tshuaj tiv thaiv kab mob affinity txiav txim siab ntawm qib cell.Countstar cov cuab yeej yog cov duab raws li cytometry, tuaj yeem pab saib xyuas los ntawm R & D mus rau cov txheej txheem tsim khoom thiab xyuas kom muaj kev tsim tawm thiab sib xws.
Cell suav thiab Viability los ntawm Trypan Blue Staining Principple
Saib xyuas thiab txheeb xyuas kab lis kev cai ntawm tes nrog cov kev daws teeb meem hauv lub xeev.Kev saib xyuas kom ntseeg tau thiab ua tau zoo yog qhov tseem ceeb rau kev ua kom cov txiaj ntsig zoo thiab cov khoom lag luam zoo vim tias txawm tias kev hloov pauv me me hauv bioprocess tsis tuaj yeem cuam tshuam rau kev ua haujlwm ntawm koj cov kab lis kev cai ntawm tes.Cell suav thiab muaj peev xwm ua tau yog qhov tseem ceeb tshaj plaws, Countstar Altair muab cov khoom siv ntse heev thiab ua raws li cGMP cov tshuaj rau cov no.
Lub Countstar Altair yog tsim los ntawm lub classic Trypan Blue exclusion txoj cai, integrating the advanced "fix focus" optical imaging bench, the most advanced cell recognition technologies, and software algorithms.Pab kom tau txais cov ntaub ntawv ntawm cell concentration, viability, aggregation rate, roundness, and diameter distribution by one run.
Viability thiab GFP Transfection Determination hauv Cells
Thaum lub sij hawm bioprocess, GFP feem ntau yog siv los fuse nrog recombinant protein raws li qhov taw qhia.Txiav txim siab GFP fluorescent tuaj yeem cuam tshuam txog lub hom phiaj qhia cov protein.Countstar Rigel muab kev ntsuam xyuas sai thiab yooj yim rau kev kuaj GFP kev hloov pauv nrog rau kev muaj peev xwm.Cells tau stained nrog Propidium iodide (PI) thiab Hoechst 33342 los txhais cov neeg tuag ntawm tes thiab tag nrho cov cell cell.Countstar Rigel muaj txoj hauv kev ceev, ntau npaum li cas rau kev ntsuas GFP kev nthuav qhia kev ua tau zoo thiab muaj txiaj ntsig zoo tib lub sijhawm.
Cells nyob siv Hoechst 33342 (xiav) thiab feem pua ntawm GFP qhia cov hlwb (ntsuab) tuaj yeem txiav txim siab tau yooj yim.Cov cell tsis tuaj yeem yog xim av nrog propidium iodide (PI; liab).
Affinity ntawm antibody nrhiav pom ntawm Countstar Rigel
Diluted cov tshuaj tiv thaiv rau hauv ntau qhov sib txawv, tom qab ntawd incubated nrog cov hlwb.Cov txiaj ntsig tau txais los ntawm Countstar Rigel (ob qho tib si duab thiab cov txiaj ntsig ntau)
Countstar yog GMP-npaj rau 21 CFR Part 11
Countstar cov cuab yeej ua raws li 21 CFR thiab Tshooj 11, cov kev pabcuam IQ/OQ/PQ ua kom muaj kev tswj xyuas kev ua haujlwm zoo ib yam.Countstar cov cuab yeej tau npaj ua tiav hauv GMP thiab 21 CFR ntu 11 cov chaw soj nstuam ua raws.Cov neeg siv kev tswj hwm thiab kev tshawb xyuas txoj hauv kev tso cai rau cov ntaub ntawv tsim nyog ntawm kev siv nrog cov ntawv tshaj tawm PDF.
IQ/OQ cov ntaub ntawv thiab cov ntaub ntawv pov thawj
