Igazi kunye neeseli ezingundoqo ezisandula ukuba zodwa okanye iiseli ezikhulisiweyo zinokuqulatha ukungcola, iintlobo ezininzi zeeseli okanye amasuntswana aphazamisayo anjengobutyobo beeseli eziya kwenza ukuba kube nzima ukuhlalutya iiseli zomdla.I-Countstar FL enohlalutyo lweendlela ezimbini ze-fluorescence inokukhuphela ngaphandle amaqhekeza eseli, i-debris kunye namasuntswana ezinto zakudala kunye neziganeko ezinobungakanani obuphantsi njengeeplatelet, ezinika iziphumo ezichaneke kakhulu.
AO/PI Dual Fluorescence Viability Ukubala
IAcridine orenji (AO) kunye nePropidium iodide (PI) ziidayi ezibophelelayo kwinucleic acid.Uhlalutyo alubandakanyi amaqhekeza eeseli, i-debris kunye ne-artifacts particles kunye neziganeko ezincinci ezifana ne-red blood cell, enika umphumo ochanekileyo.Ukuqukumbela, inkqubo ye-Countstar inokusetyenziselwa inyathelo ngalinye lenkqubo yokwenziwa kweeseli.
Ii-WBCs kwiGazi Elipheleleyo
Umzobo we-2 umfanekiso wesampulu yegazi lonke uthathwe ngu-Countstar Rigel
Ukuhlalutya ii-WBCs kwigazi elipheleleyo luvavanyo lwesiqhelo kwilebhu yezonyango okanye kwibhanki yegazi.Ukugxila kunye nokusebenza kwee-WBCs zisalathiso esibalulekileyo njengolawulo lomgangatho wokugcinwa kwegazi.
I-Countstar Rigel ene-AO/PI indlela inokwahlula ngokuchanekileyo imeko ephilayo nefileyo yeeseli.I-Rigel inokwenza ukubala kwe-WBC ngokuchanekileyo ngelixa ingabandakanyi uphazamiseko lweeseli ezibomvu zegazi.
Ukubala kunye nokusebenza kwe-PBMC
Umzobo we-3 Ummandla oqaqambileyo kunye nemifanekiso yeFluorescence ye-PBMC ibanjwe ngu-Countstar Rigel
I-AOPI Ubalo lwe-Dual-fluoresces luhlobo lovavanyo olusetyenziselwa ukufumanisa ukugxila kweeseli kunye nokusebenza.Ngenxa yoko, iiseli ze-nucleated ezinemembrane ezingaguqukiyo zingcolisa i-fluorescent eluhlaza kwaye zibalwa njengeziphilayo, ngelixa iiseli ze-nucleated ezineminwebu eyonakeleyo zibala umbala obomvu we-fluorescent kwaye zibalwa njengezifileyo xa kusetyenziswa inkqubo ye-Countstar Rigel.Izinto ezingenayo i-nucleated ezifana neeseli ezibomvu zegazi, iiplatelets kunye ne-debris azikhuli kwaye azihoywa yi-software ye-Countstar Rigel.
