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Countstar BioFerm

Countstar automated fungi ukumiswa kweseli analyzer

I-Countstar BioFerm i-automated fungi cell analyzer idibanisa iindlela ze-classical staining usebenzisa i-Methylene Blue, i-Trypan Blue, i-Methylene Violet, okanye i-Erythrosin B kunye ne-high-resolution imaging.Ii-algorithms zokuqatshelwa komfanekiso ophucukileyo zihambisa ngokuchanekileyo kunye nobhaqo oluchanekileyo lweeseli zefungi ezisebenzayo nezifileyo, ukugxininiswa kweeseli zabo, ububanzi kunye nolwazi malunga ne-morphology kunye.Inkqubo yolawulo lwedatha enamandla igcina ngokuthembekileyo iziphumo kunye nemifanekiso kwaye ivumela ukuhlalutya kwakhona nangaliphi na ixesha.  

 

Uluhlu lweSicelo

I-Countstar BioFerm iyakwazi ukubala kwaye ihlalutye iintlobo ezininzi zeentlobo zefungi (kunye ne-aggregates yazo) kuluhlu oluphakathi kwe-2μm ukuya kwi-180μm.Kwishishini le-biofuel kunye ne-biopharma, i-Countstar BioFerm ibonakalise amandla ayo njengesixhobo esithembekileyo nesikhawulezayo sokubeka iliso kwiinkqubo zemveliso.

 

IiNzuzo zoMsebenzisi

  • Ulwazi olubanzi malunga nokungunda
    Idatha ibandakanya ulwazi malunga nokugxila, ukusebenza, ukujula, ukubumbana, kunye nesantya sokuhlanganiswa.
  • Ilungelo lethu elilodwa lomenzi wechiza "iTekhnoloji yokuJonga eFixed"
    Akukho mfuneko nangaliphi na ixesha ukulungelelanisa ugxininiso lwe-Countstar BioFerm.
  • Ibhentshi yamehlo enekhamera ye-5-megapixel yombala
    Uqinisekisa ukubonwa okutyebileyo kunye neenkcukacha zezinto eziphilayo.
  • Imodyuli yohlalutyo loQelaniso
    Ivumela ingxelo ethembekileyo malunga nomsebenzi okhulayo
  • Izinto ezisetyenziswayo ezingabizi kakhulu
    Izikhundla ezihlanu zeesampulu kwiSilayidi se-Countstar Chamber enye zinciphisa iindleko zokusebenza, inkunkuma yeplastiki, kwaye igcina ixesha lovavanyo.
  • Iinkcukacha zemveliso
  • Iinkcukacha zobuGcisa
  • Khuphela
Iinkcukacha zemveliso

 

 

Isampulu yeMifanekiso ye-Baker's Yeast Saccharomyces cerevisiae

 

Imifanekiso yegwele lombhaki Saccharomyces cerevisiae ifunyenwe nge-Countstar BioFerm. Iisampulu zathathwa kwiinkqubo ezahlukeneyo zokwenziwa, ngokuyinxenye zineMethylene Blue (ezantsi ekhohlo) kunye neMethylene Violet (ekunene ezantsi)

 

 

 

Saccharomyces cerevisiae kwizigaba ezahlukeneyo zenkqubo ye-2-step fermentation

 

Phezulu ekhohlo: Icandelo lomfanekiso we-Countstar BioFerm obonisa inkcubeko yokuqalisa, engcoliswe yiMethylene Blue (MB).Isampuli iqulethe ubuninzi beeseli eziphezulu kunye neeseli zisebenza kakhulu (umlinganiselo wokufa <5%).Ngasekhohlo ngasezantsi: Isampulu engacocekanga evela kwi-bioreactor esandula kugotywa;amathupha abonakala ngokucacileyo.Ngasezantsi ngasekunene: Isampuli ithathwe kwinqanaba lokugqibela lenkqubo yokuvutshelwa ephambili, ifakwe i-1: 1 nge-MB (umlinganiselo wokufa: 25%).Iintolo ezibomvu ziphawula iiseli ezifileyo, ezibandakanya idayi ye-MB, ekhokelela kumbala omnyama wevolume yonke yeseli.

 

 

 

Ukuthelekisa idatha yomlinganiselo

 

Le mizobo ingasentla ibonisa ukuthelekiseka kwe-Countstar BioFerm ekubaleni ngesandla, kunye neenguqu ezibalulekileyo ezisezantsi kwiziphumo zomlinganiselo, ukuba kuthelekiswa nezibalo ze-hemocytometer yezandla.

 

Ukuthelekisa uhlalutyo lwe-manuwali kunye ne-athomathikhi yokusabalalisa ububanzi

 

 

Imizobo engasentla ibonisa ukuchaneka okuphezulu kwemilinganiselo yedayamitha ye-Countstar BioFerm kwisaveyi yesandla kwihemocytometer.Njengoko kwi-manual ibala inani eliphantsi le-100 leeseli lihlalutyiweyo, ipateni yokusabalalisa ububanzi iyahluka kakhulu kune-Countstar BioFerm, apho phantse i-3,000 yeeseli zeyeast zahlalutywa.

 

 

 

Ukuveliswa kwakhona kokubalwa kweeseli kunye nezinga lokufa

 

25 iialiquots ezixutyiweyo Saccharomyces cerevisiae iisampulu, eziqulethe i-concentration yegama le-6.6 × 106 iiseli / mL zahlalutywa ngokuhambelana ne-Countstar BioFerm kunye ne-hemocytometer ngesandla.

Zombini iigrafiki zibonisa umahluko ophezulu kakhulu kumanani eseli enye, olwenziwa ngesandla kwihemocytometer.Ngokwahlukileyo, i-Countstar BioFerm ihluka kancinci ukusuka kwixabiso legama lokugxila (ekhohlo) kunye nokufa (ekunene).

 

Saccharomyces cerevisiae kwizigaba ezahlukeneyo zenkqubo ye-2-step fermentation

 

Saccharomyces cerevisiae, idyojwe nguMethylene Violet kwaye emva koko yahlalutywa nge-Countstar Inkqubo yeBioFerm

Khohlo: Icandelo lomfanekiso ofunyenweyo we-Countstar Bioferm Kunene: Icandelo elifanayo, iiseli ezibhalwe yi-Countstar BioFerm ukuqondwa umfanekiso algorithms.Iiseli ezisebenzayo zijikelezwe zizangqa eziluhlaza, iiseli ezinamabala (ezifileyo). iphawulwe ngezangqa ezityheli (ibonakaliswe ngakumbi kule ncwadana ngeentolo ezityheli).Idityanisiwe iiseli zijikelezwe zizangqa ezipinki.Inani eliphezulu lee-aggregates ezimbini zeeseli ziyabonakala - isalathisi esicacileyo somsebenzi okhulayo wale nkcubeko, iintolo eziphuzi, ezifakwe ngesandla, ziphawula iiseli ezifileyo.

 

Ihistogram edityanisiweyo yokuvutshelwa kwegwele elikhula ngokugqithisileyo inqanaba eliphezulu lomsebenzi wokuhluma, ebonisa ubukhulu becala iiseli ezi-2 ezidityanisiweyo,

Iinkcukacha zobuGcisa

 

 

Iinkcukacha zobuGcisa
Imveliso yedatha Ukugxila, Ukubhubha, Idayamitha, iRate yokuHlanganisa, ukuBumbana
Uluhlu loMlinganiso 5.0 x 10 4 – 5.0 x 10 7 /ml
Ubungakanani Uluhlu 2 – 180 μm
Umthamo wegumbi 20 μl
Ixesha lomlinganiselo <20 Imizuzwana
ifomathi yesiphumo JPEG/PDF/Excel spreadsheet
Umsebenzi 5 Iisampulu / Countstar Chamber Slide

 

 

IiNgcaciso zesilayidi
Izinto eziphathekayo Poly-(Methyl) Methacrylate (PMMA)
Imilinganiselo: 75 mm (w) x 25 mm (d) x 1.8 mm (h)
Ubunzulu beGumbi: 190 ± 3 μm (kuphela yi-1.6% ukutenxa kubude ngokuchaneka okuphezulu)
Umthamo wegumbi 20 μl

 

 

Khuphela
  • Countstar BioFerm Brochure.pdf Khuphela

    • Khuphela iFayile

    • 7

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