Maselo a nyamakazi amagwiritsidwa ntchito kwambiri mu Biopharmaceuticals, monga ma antibodies, katemera, peptides ndi metabolites yachiwiri amapangidwa ndi bioprocessing ndi maselo a mammalian.Munthawi yonseyi kuchokera ku R&D ya antibody mpaka kupanga, pali njira zambiri zomwe zimafunikira kuti muyesere kutengera ma cell kuti muwunikire njira kapena kuwongolera khalidwe.Monga kuchuluka kwa ma cell ndi kuthekera kumatanthawuza momwe chikhalidwe cha cell chilili.Komanso ma cell transfection, kugwirizana kwa ma antibodies kumatsimikizira pamlingo wa cell.Zida za Countstar ndizojambula zochokera ku cytometry, zitha kuthandizira kuwunika kuchokera ku R&D kupita ku njira zopangira ndikuwonetsetsa kuberekana komanso kusasinthika.
Kuwerengera Kwa Ma Cell ndi Viability ndi Trypan Blue Staining Principle
Kuyang'anira ndi kusanthula chikhalidwe cha ma cell ndi njira zamakono.Kuwunika kodalirika komanso koyenera ndikofunikira pakukulitsa zokolola komanso mtundu wazinthu chifukwa ngakhale kusintha kwakung'ono kwa magawo a bioprocess kumatha kukhudza momwe ma cell anu amagwirira ntchito.Kuwerengera kwa ma cell ndi kuthekera kwake ndizofunikira kwambiri, Countstar Altair imapereka chidziwitso chanzeru komanso kutsatira kwathunthu cGMP yankho la izi.
The Countstar Altair idapangidwa kutengera mfundo yanthawi zonse ya Trypan Blue yopatula, kuphatikiza benchi yapamwamba ya "fix focus" yowonera, matekinoloje apamwamba kwambiri ozindikira ma cell, ndi ma algorithms apulogalamu.Yambitsani kudziwa za kuchuluka kwa maselo, kuthekera kwake, kuchuluka kwa ma aggregation, kuzungulira, ndi kugawa kwake kumodzi.
Viability ndi GFP Transfection Determination m'maselo
Panthawi ya bioprocess, GFP nthawi zambiri imagwiritsidwa ntchito kuphatikiza mapuloteni ophatikizanso ngati chizindikiro.Dziwani kuti fluorescent ya GFP imatha kuwonetsa zomwe mukufuna.Countstar Rigel imapereka kuyesa kwachangu komanso kosavuta kuyesa kufalikira kwa GFP komanso kutheka.Maselo adadetsedwa ndi Propidium iodide (PI) ndi Hoechst 33342 kuti afotokoze kuchuluka kwa maselo akufa ndi kuchuluka kwa ma cell.Countstar Rigel imapereka njira yachangu, yochulukira yowunikira magwiridwe antchito a GFP komanso kutheka nthawi imodzi.
Maselo amapezeka pogwiritsa ntchito Hoechst 33342 (buluu) ndipo kuchuluka kwa ma cell a GFP (wobiriwira) amatha kudziwitsidwa mosavuta.Maselo osatheka amakhala odetsedwa ndi propidium iodide (PI; red).
Kugwirizana kwa kudziwika kwa antibody pa Countstar Rigel
Kusungunula antibody mu ndende zosiyanasiyana, ndiye incubated ndi maselo.Zotsatirazo zidapezedwa kuchokera ku Countstar Rigel (zonse chithunzi ndi kuchuluka kwake)
Countstar ndi GMP-yokonzekera 21 CFR Gawo 11
Zida za Countstar zimagwirizana kwathunthu ndi 21 CFR ndi Gawo 11, mautumiki a IQ / OQ / PQ amaonetsetsa kuti ntchitoyo ikuyendetsedwa mosasinthasintha.Zida za Countstar zakonzeka kukhazikitsidwa mu GMP ndi 21 CFR gawo 11 ma laboratories ovomerezeka.Kuwongolera kwa ogwiritsa ntchito ndi njira zowunikira zimalola zolemba zokwanira zogwiritsidwa ntchito ndi malipoti okhazikika a PDF.
Zolemba za IQ/OQ ndi magawo ovomerezeka
