Countstar Mira FL

Zogulitsa

Innovative Optical Multiplication Technology

Tekinoloje yapadera yowonera makulitsidwe imathandizira ogwiritsa ntchito kusanthula ma cell mumadiameter osiyanasiyana

Mukamagwiritsa ntchito ma tempulo owala a BioApp mu Countstar Mira, buku la Zooming Technology limathandiza wogwiritsa ntchito kuzindikira molondola zinthu zam'manja zomwe zili mkati mwa diamater kuyambira 1.0µm mpaka 180.0µm.Zithunzi zomwe zapezedwa zimawonetsanso zambiri zamaselo amodzi.Izi zimakulitsa kuchuluka kwa ntchito ngakhale kuzinthu zam'manja, zomwe sizidathe kuwunikidwa bwino m'mbuyomu.

Progressive AI based Image Analysis algorithms

Countstar Mira FL imagwiritsa ntchito maubwino a Artifical Intelligence kupanga ma aligorivimu odziphunzirira okha.Amatha kuzindikira ndi kusanthula mikhalidwe yambiri ya maselo.Kuphatikizika kwa magawo a mawonekedwe a cell kumapangitsa kusanthula kolondola kwambiri komanso kobwerezabwereza kwa momwe ma cell azungulira komanso/kapena kumapereka chidziwitso chokhudzana ndi kusintha kwa ma cell morphology, kupanga magulu a cell (aggregates, ma spheroid ang'onoang'ono) ndi zomwe zimakhudza.

Kulemba zotsatira za Mesenchymal Stem Cells (MSC; 5x mangification) mu chikhalidwe chochulukirachulukira

• Zozungulira zobiriwira zimayika maselo amoyo
• Zozungulira zofiira zimayika maselo akufa
• Magulu oyera ophatikiza maselo

RAW264.7 cell line ndi yaying'ono komanso yopindika mosavuta.The Countstar AI algorithm imatha kuzindikira ma cell omwe ali mumagulu ndikuwerengera

• Zozungulira zobiriwira zimayika maselo amoyo
• Zozungulira zofiira zimayika maselo akufa
• Magulu oyera ophatikiza maselo

Kukula kosagwirizana kwa ma cell a embryonic a zebrafish (6.6X magnification

• Zozungulira zobiriwira zimayika maselo amoyo
• Zozungulira zofiira zimayika maselo akufa
• Magulu oyera ophatikiza maselo

Kapangidwe ka Intuitive Graphical User Interface (GUI).

GUI yowoneka bwino imalola kuyesa koyenera komanso kosavuta

• Laibulale yayikulu yokhala ndi mitundu yokhazikitsidwa kale ndi BioApps (ma protocol aassay template).Kungodina kamodzi pa BioApp, ndipo mayeso angayambe.
• GUI yosavuta kugwiritsa ntchito imapangitsa kukhala kosavuta kusinthana pakati pamitundu yosiyanasiyana ya menyu ndikutsimikizira zoyeserera zomasuka
• Ma module osanjidwa bwino amathandizira wogwiritsa ntchito pamayeso atsiku ndi tsiku

Sankhani BioApp, lowetsani Chidziwitso Chachitsanzo, ndikuyamba kuyesa

128 GB ya mphamvu yapakati yosungira deta, yokwanira kusunga pafupifupi.Kusanthula kwa 50,000 kumabweretsa Countstar (R) Mira.Kuti mupeze mwachangu, deta yomwe mukufuna imatha kusankhidwa ndi zosankha zosiyanasiyana.

Chinthu chothandiza kuti musunge nthawi, ndi chowerengera chowerengera chomwe chingabwezerenso.Idzapereka mavoti enieni a maselo a diluent ndi oyambirira, kamodzi kokha ndende yomaliza ya maselo ndi voliyumu yomwe mukufuna kutsata ikalowetsedwa.Izi zimapangitsa kudutsa kwa ma cell kupita kumagulu awo kukhala omasuka.

Zambiri zamapulogalamu

Zowunikira za Countstar Mira zimathandizira wogwiritsa ntchito kumvetsetsa kusintha kwamphamvu mkati mwa chikhalidwe cha cell ndikuthandizira kukulitsa zomwe akukula.

Mapulogalamu apamwamba, a AI ozindikiritsa zithunzi a Countstar Mira amatha kupereka magawo angapo.Kupatula zotsatira zofananira za kuchuluka kwa ma cell komanso kuthekera kwake, kukula kwa maselo, kupangika kwamagulu a cell, kuchuluka kwa fluorescence kwa selo lililonse, mawonekedwe a kapindika kakukula, ndi mawonekedwe awo akunja a morphology ndizofunika kwambiri kuti awone zenizeni. chikhalidwe cha cell chikhalidwe.Ma graph omwe amapangidwa okha a ma curve, kugawa m'mimba mwake ndi ma fluorescence intensity histograms, kusanthula kwa cell imodzi mkati mwamagulu ophatikizika komanso kutsimikiza kwa gawo la cell compactness kumathandizira wosuta kuti amvetsetse bwino zomwe zimachitika mkati mwa cell yoyesedwa kuyambira koyambira mpaka kumapeto kwa ntchitoyi.

Histogram

Relative Fluorescence Intensity (RFI) yogawa histogram

Diameter yogawa histogram

Mzere wa kukula

Zithunzi Zoyesa ndi Zotsatira

Chithunzi cha kukula kwa curve

Product Application

AO/PI dual fluorescence cell density and viability assays

Njira yodetsa yamitundu iwiri ya fluorescence AO/PI imachokera pa mfundo yakuti mitundu yonse iwiri ya utoto, Acridine Orange (AO) ndi Propidium Iodide (PI), imagwirizana pakati pa nucleic acids ya chromosome mu nyukiliyasi ya selo.Ngakhale AO imatha kulowa m'mitsempha ya nyukiliya nthawi iliyonse ndikudetsa DNA, PI imatha kudutsa nembanemba yosokonekera ya nyukiliya ya cell yakufa (yakufa).AO yochuluka mu cell nucleus imatulutsa kuwala kobiriwira pamtunda wa 525nm, ngati kukondwera ndi 480nm, PI ikutumiza kuwala kofiira ndi matalikidwe ake pa 615nm, pamene ikusangalala ndi 525nm.Mphamvu ya FRET (Foerster Resonance Energy Transfer) imatsimikizira, kuti chizindikiro chotulutsidwa cha AO pa 525nm chimalowetsedwa pamaso pa utoto wa PI kupewa kutulutsa kowirikiza kawiri ndikutaya.Kuphatikizika kwa utoto wapadera wa AO/PI kumeneku kumalola kusefa makamaka ma cell okhala ndi ma acaryotes ngati erythrocyte.

Deta ya Countstar Mira FL inawonetsa mzere wabwino wa kuchepetsedwa kwa ma cell a HEK293

GFP/RFP kusanthula magwiridwe antchito

Kugwira ntchito bwino kwa ma transfection ndi index yofunikira pakukula kwa ma cell ndi kukhathamiritsa, pakukonza ma viral vector, komanso kuwunikira zokolola mumayendedwe a Biopharma.Chakhala chiyeso chomwe chimakhazikitsidwa pafupipafupi kuti adziwe mwachangu zomwe zili mu protein yomwe mukufuna kulowa mkati mwa cell.M'njira zosiyanasiyana zochizira ma jini, ndi chida chofunikira kwambiri chowongolera kusinthika kwamtundu womwe mukufuna.

Countstar Mira sikuti imangopereka zotsatira zolondola komanso zolondola, poyerekeza ndi cytometry yoyenda, kuwonjezeranso kusanthula kumapereka zithunzi ngati umboni wa umboni.Kupatula izi, imathandizira kwambiri ndikufulumizitsa kusanthula kuti kuwongolera chitukuko cha chitukuko ndi kupanga.

Zithunzi zojambulidwa, zopezedwa ndi Countstar(R) Mira, kuwonetsa kuchuluka kwa magwiridwe antchito (kuchokera kumanzere kupita kumanja) kwa maselo osinthidwa ma genetic (HEK 293 cell line; kufotokoza GFP mosiyanasiyana)

Zotsatira za miyeso yofananira, yochitidwa ndi B / C CytoFLEX, kutsimikizira deta yogwira ntchito ya GFP ya maselo osinthidwa a HEK 293, kufufuzidwa mu Countstar Mira.

Kusanthula kokhazikika kwa Trypan Blue

Kuyesa tsankho la Trypan Blue ndi njira imodzi yomwe imagwiritsidwa ntchito kwambiri komanso yodalirika yodziwira kuchuluka kwa maselo (akufa) omwe ali mkati mwa chikhalidwe choyimitsidwa cha cell.Maselo otheka okhala ndi nembanemba yakunja ya cell amathamangitsa Trypan Blue kuti asalowe mu nembanemba.Ngati, nembanemba ya cell imatuluka chifukwa cha kufa kwa cell yake, Trypan Blue imatha kudutsa chotchinga cha membrane, imadziunjikira mu plasma ya cell ndikudetsa buluu.Kusiyana kwa kuwalaku kungagwiritsidwe ntchito kusiyanitsa maselo amoyo osasinthika kuchokera ku maselo akufa ndi ma aligorivimu ozindikira zithunzi a Countstar Mira FL.

• Zithunzi za atatu, mizere yamtundu wa Trypan Blue, yopezeka mu Countstar (R) Mira FL mumayendedwe owala.

• Zotsatira za dilution gradient ya HEK 293 mndandanda