Kunyumba » Za CAR-T Cell Therapy

Za CAR-T Cell Therapy

  • 1.Zosonkhanitsa
  • 2.Kudzipatula
  • 3.Kusintha
  • 4.Kukula
  • 5.Kukolola
  • 6. Product QC
  • 7. Chithandizo

Zomwe tingachite

  • AO/PI Viability
  • Cell Cytotoxicity
  • Kusintha Mwachangu
  • Cell Apoptosis
  • Cell Cycle
  • CD Marker
  • Maselo Owonongeka
  • Kuwerengera Maselo
  • Ma cell Line
AO/PI Viability
AO/PI Viability

Dual-fluorescence Viability(AO/PI), Acridine orange (AO) ndi propidium iodide (PI) ndi utoto wa nyukiliya wa nucleic ndi utoto womanga asidi.AO imatha kulowa mu nembanemba ya onse akufa ndi maselo amoyo ndikudetsa phata, kupanga wobiriwira fluorescence.Mosiyana ndi izi, PI imatha kulowa m'mitsempha yosokonekera ya ma cell okhala ndi nucleated, kupanga fluorescence yofiira.Ukadaulo wopangidwa ndi zithunzi za Countstar Rigel suphatikiza zidutswa za cell, zinyalala, ndi tinthu tating'onoting'ono komanso zochitika zochepa monga mapulateleti, zomwe zimapereka zotsatira zolondola kwambiri.Pomaliza, dongosolo la Countstar Rigel litha kugwiritsidwa ntchito pagawo lililonse la kupanga ma cell.

Cell Cytotoxicity
Cell Cytotoxicity

T/NK Cell-Mediated Cytotoxicity, Mu chithandizo cha posachedwapa chovomerezedwa ndi FDA cha CAR-T, ma T-lymphocyte opangidwa ndi majini amamanga makamaka ku maselo a khansa (T) ndikuwapha.The Countstar Rigel analyzers amatha kusanthula ndondomeko yonseyi ya T / NK Cell-Mediated Cytotoxicity.

Maphunziro a cytotoxicity amachitidwa polemba ma cell a khansa omwe akutsata ndi CFSE kapena kuwapatsira ndi GFP.Hoechst 33342 ikhoza kugwiritsidwa ntchito kudetsa ma cell onse (ma T cell ndi chotupa).Kapenanso, ma cell chotupa omwe amayang'ana amatha kuipitsidwa ndi CFSE.Propidium iodide (PI) imagwiritsidwa ntchito kuwononga maselo akufa (ma T cell ndi ma cell chotupa).Kusankhana pakati pa maselo osiyanasiyana kungapezeke pogwiritsa ntchito njira yodetsa iyi.

Transfection Efficiency
Kusintha Mwachangu

GFP Transfection Efficiency, Mu genetics ya maselo, zamoyo zosiyanasiyana, ndi biology yama cell, jini ya GFP imagwiritsidwa ntchito nthawi zambiri ngati mtolankhani pamaphunziro ofotokozera.Pakadali pano, asayansi amagwiritsa ntchito ma microscopes a fulorosenti kapena ma cytometer oyenda kuti aunike momwe ma cell a mammalian amatha kufalikira.Koma kusamalira ukadaulo wovuta wa cytometer yopita patsogolo kumafuna munthu wodziwa zambiri komanso wodziwa bwino ntchito.Countstar Rigel imathandizira ogwiritsa ntchito kuyesa mosavuta komanso molondola popanda kugwiritsa ntchito ndi kukonza ndalama zomwe zimayenderana ndi cytometry yachikhalidwe.

Cell Apoptosis
Cell Apoptosis

Cell Apoptosis, Kuyenda kwa cell apoptosis kumatha kuyang'aniridwa pogwiritsa ntchito FITC conjugated Annexin-V kuphatikiza 7-ADD.Zotsalira za Phosphatidylserine (PS) nthawi zambiri zimakhala mkati mwa nembanemba ya plasma ya maselo athanzi.Kumayambiriro kwa apoptosis, kukhulupirika kwa nembanemba kumatayika ndipo PS imasamutsidwa kupita kunja kwa nembanemba ya cell.Annexin V imagwirizana kwambiri ndi PS motero ndiye chikhomo choyenera pama cell a apoptotic.

Cell Cycle
Cell Cycle

Ma cell Cycle, Pakugawanika kwa maselo, maselo amakhala ndi kuchuluka kwa DNA.Cholembedwa ndi PI, kuwonjezeka kwa mphamvu ya fluorescence kumagwirizana mwachindunji ndi kudzikundikira kwa DNA.Kusiyanasiyana kwa fluorescence intensity wa maselo limodzi ndi zizindikiro za udindo weniweni wa selo mkombero MCF 7 maselo ankachitira ndi 4μM wa Nocodazole kumanga maselo awa pa magawo osiyanasiyana a mkombero selo.Zithunzi zowala zopezeka panthawi yoyesererazi zimatilola kuzindikira selo lililonse.Njira ya PI fluorescence ya Countstar Rigel imazindikiritsa zizindikiro za DNA zamaselo amodzi ngakhale pamagulu.Kusanthula mwatsatanetsatane kwa mphamvu ya fluorescence kutha kuchitidwa pogwiritsa ntchito FCS.

CD Marker
CD Marker

CD Marker Phenotyping, Mitundu ya Countstar Rigel imapereka njira yofulumira, yosavuta komanso yachidziwitso cha, immuno-based phenotyping yama cell bwino.Ndi zithunzi zowoneka bwino komanso luso lophatikizika lophatikizika la data, Countstar Rigel imalola ogwiritsa ntchito kupeza zotsatira zodalirika popanda kufunikira kowongolera zovuta komanso zosintha zamalipiro a fluorescence.

Kusiyanitsa kwa ma cell a Cytokine Induced Killer (CIK) kukuwonetsa magwiridwe antchito apamwamba a Countstar Rigel analyzer poyerekeza mwachindunji ndi ma cytometer othamanga kwambiri.PBMCs ya mbewa mu chikhalidwe inali yodetsedwa ndi CD3-FITC, CD4-PE, CD8-PE, ndi CD56-PE, ndipo inayambitsidwa ndi Interleukin (IL) 6. Kenaka anafufuzidwa nthawi imodzi ndi Countstar® Rigel ndi Flow Cytometry.Pachiyeso ichi, CD3-CD4, CD3-CD8, ndi CD3-CD56 adagawidwa m'magulu atatu, kuti adziwe kuchuluka kwa maselo osiyanasiyana.

Degenerated Cells
Maselo Owonongeka

Kuzindikira Maselo Owonongeka ndi Immunofluorescence, ma antibodies a Monoclonal omwe amapanga ma cell amataya ma clones abwino panthawi yakukula kwa maselo ndikudutsa chifukwa cha kuwonongeka kapena kusintha kwa ma genetic.Kutayika kwakukulu kudzakhudza kwambiri zokolola za kupanga.Kuyang'anira kuwonongeka kumagwira ntchito yofunika kwambiri pakuwongolera njira kuti asinthe zokolola za ma antibodies kuti zikhale momwe zilili.

Ma antibodies ambiri opangidwa mumakampani a BioPharma amatha kuzindikirika ndi zilembo za immunofluorescence ndikuwunikidwa mochulukira ndi mndandanda wa Countstar Rigel.Zithunzi zowala komanso zamtundu wa fluorescence pansipa zikuwonetsa bwino ma clones omwe adataya mawonekedwe awo kuti apange ma antibodies omwe akufuna.Kusanthula mwatsatanetsatane ndi pulogalamu ya DeNovo FCS Express Image kumatsimikizira kuti 86.35 % ya maselo onse akuwonetsa ma immunoglobulins, 3.34% okha ndi omwe alibe.

Cell Counting
Kuwerengera Maselo

Trypan (capitalize B mu Buluu) Kuwerengera Ma cell, madontho a buluu a Trypan amagwiritsidwabe ntchito m'ma lab ambiri azikhalidwe zama cell.

Trypan Blue Viability ndi Cell Density BioApp ikhoza kukhazikitsidwa pamitundu yonse ya Countstar Rigel.Ma algorithms athu otetezedwa ozindikira zithunzi amasanthula magawo opitilira 20 kuti asankhe chinthu chilichonse chomwe chapezeka.

Cell Line
Ma cell Line

Cell Line Storage QC, Mu kusungirako ma cell, lingaliro laukadaulo la kasamalidwe kabwino limatsimikizira kuwunika kotetezeka, koyenera kwazinthu zonse zam'manja.Izi zimatsimikizira kukhazikika kwa cell cryopreserved, cryo-yosungidwa kuti iyesedwe, kakulidwe kazinthu, ndi kupanga.

The Countstar Rigel amapeza zithunzi zowoneka bwino, kusanthula mawonekedwe osiyanasiyana azinthu zama cell monga mainchesi, mawonekedwe, ndi chizolowezi chophatikiza.Zithunzi zosiyana ndondomeko masitepe mosavuta poyerekeza wina ndi mzake.Chifukwa chake kusiyanasiyana kwamawonekedwe ndi kuphatikizika kumatha kuzindikirika mosavuta, popewa miyeso yamunthu.Ndipo nkhokwe ya Countstar Rigel ili ndi kasamalidwe kake kakusungirako ndi kubweza zithunzi ndi deta.

Zoperekedwa

Zothandizira Zogwirizana

Zinsinsi zanu ndizofunikira kwa ife.

Timagwiritsa ntchito makeke kuti tikulitse zomwe mumakumana nazo mukamayendera mawebusayiti athu: ma cookie akugwira ntchito amatiwonetsa momwe mumagwiritsira ntchito tsambali, ma cookie ogwira ntchito amakumbukira zomwe mumakonda komanso kutsata ma cookie amatithandiza kugawana zomwe zikugwirizana ndi inu.

Landirani

Lowani muakaunti