Dual-fluorescence Viability (AO / PI), Acridine txiv kab ntxwv (AO) thiab propidium iodide (PI) yog cov xim ntawm cov nucleic thiab cov kua qaub-khi dyes.AO tuaj yeem nkag mus rau hauv daim nyias nyias ntawm ob lub cell tuag thiab muaj sia nyob thiab stains lub nucleus, tsim cov fluorescence ntsuab.Nyob rau hauv sib piv, PI tsuas tuaj yeem permeate lub disintegrating daim nyias nyias ntawm cov nucleated hlwb tuag, tsim cov fluorescence liab.Cov duab-raws li thev naus laus zis ntawm Countstar Rigel tsis suav nrog cov khoom tawg ntawm tes, cov khib nyiab, thiab cov khoom siv dag zog nrog rau cov xwm txheej tsis zoo xws li platelets, muab cov txiaj ntsig zoo.Hauv kev xaus, Countstar Rigel system tuaj yeem siv rau txhua kauj ruam ntawm cov txheej txheem ntawm tes.

T / NK Cell-Mediated Cytotoxicity, Nyob rau hauv tsis ntev los no FDA-pom zoo CAR-T cell kho, genetic engineered T-lymphocytes khi tshwj xeeb rau cov hom qog nqaij hlav (T) thiab tua lawv.Countstar Rigel analyzers muaj peev xwm txheeb xyuas cov txheej txheem tiav ntawm T / NK Cell-Mediated Cytotoxicity.

Cov kev tshawb fawb cytotoxicity yog ua los ntawm kev sau cov kab mob qog noj ntshav nrog CFSE lossis hloov lawv nrog GFP.Hoechst 33342 tej zaum yuav siv los stain tag nrho cov hlwb (ob leeg T hlwb thiab qog hlwb).Xwb, lub hom phiaj qog hlwb tuaj yeem stained nrog CFSE.Propidium iodide (PI) yog siv los stain hlwb tuag (ob leeg T hlwb thiab qog hlwb).Kev ntxub ntxaug ntawm cov hlwb sib txawv tuaj yeem tau txais los ntawm kev siv lub tswv yim staining no.

GFP Transfection Efficiency, Nyob rau hauv molecular noob caj noob ces, ntau yam qauv kab mob, thiab cell biology, GFP noob yog nquag siv raws li ib tug reporter rau qhia kev kawm.Tam sim no, cov kws tshawb fawb feem ntau siv cov fluorescent microscopes los yog cov cytometers ntws los txheeb xyuas cov txiaj ntsig ntawm kev hloov pauv ntawm cov tsiaj txhu.Tab sis tuav txoj kev siv thev naus laus zis ntawm kev ua haujlwm siab cytometer xav tau tus neeg ua haujlwm paub txog thiab tsim nyog.Countstar Rigel ua rau cov neeg siv tau yooj yim thiab raug ua qhov kev ntsuam xyuas kev ua haujlwm zoo yam tsis muaj kev ua haujlwm thiab kev saib xyuas cov nqi cuam tshuam nrog cov tshuaj cytometry.

Cell Apoptosis, Kev nce qib ntawm cell apoptosis tuaj yeem saib xyuas siv FITC conjugated Annexin-V ua ke nrog 7-ADD.Phosphatidylserine (PS) residues feem ntau nyob rau sab hauv ntawm cov plasma membrane ntawm cov hlwb noj qab haus huv.Thaum lub sij hawm apoptosis thaum ntxov, daim nyias nyias tau ploj mus thiab PS yuav raug hloov mus rau sab nraud ntawm cov cell membrane.Annexin V muaj kev sib raug zoo rau PS thiab yog li ntawd yog lub cim zoo rau cov hlwb apoptotic thaum ntxov.

Cell Cycle, Thaum lub sij hawm faib cell, cov hlwb muaj cov DNA ntau ntxiv.Sau npe los ntawm PI, qhov nce hauv fluorescence siv ncaj qha ncaj qha rau kev sib sau ntawm DNA.Qhov sib txawv ntawm cov fluorescence intensities ntawm tib lub hlwb yog cov cim qhia ntawm cov xwm txheej ntawm lub voj voog ntawm tes MCF 7 hlwb tau kho nrog 4μM ntawm Nocodazole kom ntes cov hlwb ntawm ntau theem ntawm lawv lub voj voog ntawm tes.Cov duab ci ntsa iab uas tau txais thaum lub sijhawm sim no tso cai rau peb txheeb xyuas txhua lub xov tooj ntawm tes.PI fluorescence channel ntawm Countstar Rigel txheeb xyuas cov cim DNA ntawm tib lub hlwb txawm tias nyob hauv cov khoom sib sau.Kev soj ntsuam ntxaws ntxaws ntawm cov fluorescence intensities tuaj yeem ua tiav siv FCS.

CD Marker Phenotyping, Countstar Rigel qauv muab kev nrawm dua, yooj yim dua thiab yooj yim dua mus rau, immuno-based phenotyping ntawm cov hlwb ntau dua.Nrog cov duab daws teeb meem siab thiab muaj peev xwm ua ke nrog cov ntaub ntawv txheeb xyuas muaj peev xwm, Countstar Rigel tso cai rau cov neeg siv kom ua tiav cov txiaj ntsig zoo tsis tu ncua yam tsis tas yuav tsum muaj kev tswj xyuas qhov nyuaj thiab kev hloov kho fluorescence.

Cytokine Induced Killer (CIK) ntawm tes sib txawv ua kom pom qhov ua tau zoo ntawm Countstar Rigel analyzer hauv kev sib piv ncaj qha rau cov chav kawm siab cytometers.PBMCs ntawm nas hauv kab lis kev cai tau stained nrog CD3-FITC, CD4-PE, CD8-PE, thiab CD56-PE, thiab induced los ntawm Interleukin (IL) 6. Tom qab ntawd soj ntsuam ib txhij nrog Countstar® Rigel thiab Flow Cytometry.Hauv qhov kev sim no, CD3-CD4, CD3-CD8, thiab CD3-CD56 tau muab faib ua peb pawg, txhawm rau txiav txim siab qhov sib txawv ntawm cov cell subpopulations.

Kev kuaj pom ntawm Degenerated Cells los ntawm Immunofluorescence, Monoclonal antibodies uas tsim cov kab ntawm tes yuav poob qee qhov zoo clones thaum lub sij hawm cell proliferation thiab dhau mus vim degradation los yog noob caj noob ces.Kev poob ntau dua yuav cuam tshuam rau kev tsim khoom ntawm cov txheej txheem tsim khoom.Kev saib xyuas ntawm qhov degradation plays lub luag haujlwm tseem ceeb hauv cov txheej txheem tswj kom hloov cov txiaj ntsig ntawm cov tshuaj tiv thaiv mus rau qhov zoo tshaj plaws.

Feem ntau ntawm cov tshuaj tiv thaiv tsim nyob rau hauv BioPharma kev lag luam tuaj yeem kuaj pom los ntawm cov tshuaj tiv thaiv kab mob fluorescence thiab tshuaj xyuas kom muaj nuj nqis los ntawm Countstar Rigel series.Cov duab ci ntsa iab thiab fluorescence channel cov duab hauv qab no qhia meej cov clones uas poob lawv cov cwj pwm los tsim cov tshuaj tiv thaiv uas xav tau.Qhov kev soj ntsuam ntxaws ntxiv nrog DeNovo FCS Express Image software lees paub, tias 86.35% ntawm tag nrho cov hlwb tau qhia txog cov tshuaj tiv thaiv kab mob, tsuas yog 3.34% yog qhov tsis zoo.

Trypan (capitalize B hauv Blue) Cell suav, Trypan xiav staining tseem siv nyob rau hauv feem ntau ntawm cell kab lis kev cai labs.

Lub Trypan Blue Viability thiab Cell Density BioApp tuaj yeem ntsia tau rau txhua tus qauv Countstar Rigel.Peb cov duab tiv thaiv kev paub txog algorithms tsom xam ntau dua 20 qhov tsis muaj los txheeb xyuas txhua qhov khoom pom.

Cell Line Storage QC, Hauv kev cia ntawm tes, lub tswv yim zoo tswj xyuas kom muaj kev nyab xeeb, saib xyuas txhua yam khoom ntawm tes.Qhov no lav qhov ruaj khov zoo ntawm cov xov tooj ntawm tes cryopreserved, cryo-preserved rau kev sim, txheej txheem kev loj hlob, thiab ntau lawm.

Lub Countstar Rigel tau txais cov duab daws teeb meem siab, tshuaj xyuas ntau yam morphological yam ntxwv ntawm cov khoom ntawm tes xws li txoj kab uas hla, cov duab, thiab kev sib sau ua ke.Cov duab ntawm cov txheej txheem sib txawv tuaj yeem yooj yim piv rau ib leeg.Yog li kev hloov pauv hauv cov duab thiab kev sib sau tuaj yeem pom tau yooj yim, los ntawm kev zam kev ntsuas tib neeg.Thiab Countstar Rigel database muaj cov txheej txheem kev tswj hwm zoo rau kev khaws cia thiab khaws cov duab thiab cov ntaub ntawv.