Mesenchymal qia hlwb yog ib feem ntawm pluripotent qia hlwb uas tuaj yeem raug cais tawm ntawm mesoderm.Nrog rau lawv tus kheej-replication rov ua dua tshiab thiab ntau txoj kev sib txawv ntawm cov yam ntxwv, lawv muaj peev xwm rau ntau yam kev kho mob hauv cov tshuaj.Mesenchymal qia hlwb muaj qhov tshwj xeeb tiv thaiv kab mob phenotype thiab kev tiv thaiv kab mob muaj peev xwm.Yog li ntawd, mesenchymal qia hlwb twb tau siv dav hauv kev hloov pauv ntawm cov qia, cov ntaub so ntswg engineering thiab kev hloov pauv hauv nruab nrog cev.Thiab dhau ntawm cov ntawv thov no, lawv tau siv los ua cov cuab yeej zoo tshaj plaws hauv kev tsim cov ntaub so ntswg ua cov noob qoob loo hauv cov kev tshawb fawb hauv kev sim thiab kev sim tshuaj.
Lub Countstar Rigel tuaj yeem saib xyuas qhov concentration, kev muaj peev xwm, kev tshuaj ntsuam apoptosis thiab cov yam ntxwv phenotype (thiab lawv cov kev hloov pauv) thaum lub sijhawm tsim khoom thiab sib txawv ntawm cov qia hlwb.Lub Countstar Rigel kuj tseem muaj qhov zoo dua hauv kev tau txais cov ntaub ntawv morphological ntxiv, muab los ntawm qhov chaw ci ntsa iab mus tas li thiab cov duab thaij duab fluorescence thaum lub sijhawm tag nrho cov txheej txheem ntawm kev saib xyuas ntawm tes.Lub Countstar Rigel muab txoj hauv kev nrawm, sophisticated thiab txhim khu kev qha rau kev tswj cov qia hlwb.
Saib xyuas kev muaj peev xwm ntawm MSCs hauv Cov Tshuaj Kho Mob
Daim duab 1 Kev soj ntsuam ntawm kev muaj peev xwm thiab suav ntawm tes ntawm mesenchymal qia hlwb (MSCs) rau kev siv hauv kev kho ntawm tes
Stem Cell yog ib qho ntawm cov kev kho mob zoo tshaj plaws hauv kev kho cell regenerative.Los ntawm kev sau qoob loo ntawm MSC mus rau kev kho mob, nws yog ib qho tseem ceeb kom txhawb nqa lub qia cell muaj peev xwm nyob rau hauv tag nrho cov kauj ruam ntawm qia cell ntau lawm (Daim duab 1).Countstar's qia cell txee saib xyuas qia cell viability thiab concentration los ua lub luag haujlwm tseem ceeb hauv kev tswj kom zoo.
Saib xyuas MSC Morphological Hloov Kho tom qab Kev Thauj Mus Los
Txoj kab uas hla thiab kev sib sau ua ke kuj tau txiav txim los ntawm Countstar Rigel.Txoj kab uas hla ntawm AdMSCs tau hloov pauv loj heev tom qab kev thauj mus los thaum piv nrog ua ntej kev thauj mus los.Txoj kab uas hla ua ntej kev thauj mus los yog 19µm, tab sis nws tau nce mus rau 21µm tom qab kev thauj mus los.Kev sib sau ua ntej kev thauj mus los yog 20%, tab sis nws tau nce mus rau 25% tom qab kev thauj mus los.Los ntawm cov duab uas tau ntes los ntawm Countstar Rigel, phenotype ntawm AdMSCs tau hloov pauv sai heev tom qab kev thauj mus los.Cov txiaj ntsig tau pom hauv daim duab 3.
Kev txheeb xyuas ntawm AdMSCs hauv Cell Phenotype
Tam sim no yam tsawg kawg nkaus tus txheej txheem kev txheeb xyuas cov txheej txheem rau kev ruaj ntseg zoo ntawm kev soj ntsuam MSCs tau teev nyob rau hauv ib nqe lus ntawm International Society for Cellular Therapy (ISCT), tau hais tseg nyob rau hauv 2006.
Kev Tshawb Fawb nrawm ntawm Apoptosis hauv MSCs nrog FITC Conjugated Annexin-V thiab 7-ADD Taw Qhia
Cell Apoptosis tuaj yeem kuaj pom nrog FITC conjugated annexin-V thiab 7-ADD.PS feem ntau tsuas pom muaj nyob rau ntawm daim nplooj ntawv intracellular ntawm cov plasma membrane hauv cov hlwb noj qab haus huv, tab sis thaum ntxov apoptosis, membrane asymmetry poob thiab PS hloov mus rau daim ntawv sab nraud.
Daim duab 6 Kev Tshawb Nrhiav Apoptosis hauv MSCs los ntawm Countstar Rigel
A. Kev soj ntsuam pom ntawm cov duab fluorescence ntawm Kev Tshawb Fawb ntawm Apoptosis hauv MSCs
B. Scatter cov phiaj xwm ntawm Apoptosis hauv MSCs los ntawm FCS nthuav qhia
C. Feem pua ntawm cov pej xeem ntawm tes raws li % li qub, % apoptotic, thiab % necrotic / heev lig-theem apoptotic hlwb.
