Protocol ea Teko
Cytotoxicity % e baloa ka equation e ka tlase.
Cytotoxicity% = (Lipalo tse phelang tsa taolo - Lipalo tse phelang tsa phekolo) / Lipalo tse phelang tsa taolo × 100
Ka ho tšoaea lisele tsa hlahala tse shebiloeng ka calcein AM e seng chefo, e seng radioactive kapa e fetisoa ka GFP, re ka beha leihlo polao ea lisele tsa tumor ka lisele tsa CAR-T.Le ha lisele tsa mofetše tse phelang li tla ngoloa ke calcein e tala AM kapa GFP, lisele tse shoeleng li ke ke tsa boloka dae e tala.Hoechst 33342 e sebelisoa bakeng sa letheba lisele tsohle (lisele T ka bobeli le lisele tsa hlahala), ho seng joalo, lisele tse shebiloeng hlahala ka stained le lera tlamme calcein AM, PI e sebediswa bakeng sa letheba lisele tse shoeleng (lisele T ka bobeli le lisele hlahala).Leano lena la litšila le lumella ho khetholla lisele tse fapaneng.
E: T Karo-karolelano e itšetlehileng ka Cytotoxicity ea K562
Mohlala Hoechst 33342, CFSE, PI litšoantšo tsa fluorescent ke lisele tse shebiloeng tsa K562 ho t = lihora tse 3.
Litšoantšo tsa fluorescent tse hlahisitsoeng li bonts'itse keketseho ea Hoechst+CFSE+PI+ Target cell as E: T ratio e eketsehile.
