Dual-fluorescence Viability(AO/PI), Acridine orange (AO) le propidium iodide (PI) ke nuclear nucleic staining and dyes tse tlamang asiti.AO e ka kenella ka har'a lera la lisele tse shoeleng le tse phelang 'me ea silafatsa khubu, ea hlahisa fluorescence e tala.Ka lehlakoreng le leng, PI e ka kenella feela ka har'a lera le qhalaneng la lisele tse shoeleng tsa nucleated, tse hlahisang fluorescence e khubelu.Theknoloji e thehiloeng litšoantšong ea Countstar Rigel ha e kenyeletse likotoana tsa lisele, maloanlahla le likaroloana tsa maiketsetso hammoho le liketsahalo tse nyenyane tse kang li-platelet, tse fanang ka sephetho se nepahetseng haholo.Qetellong, sistimi ea Countstar Rigel e ka sebelisoa mohatong o mong le o mong oa ts'ebetso ea ho etsa lisele.

T/NK Cell-Mediated Cytotoxicity, Phekolong ea morao tjena e amohetsoeng ke FDA ea CAR-T, T-lymphocyte e entsoeng ka liphatsa tsa lefutso e tlama ka ho khetheha liseleng tsa mofetše (T) tse lebisitsoeng ho tsona ebe lia li bolaea.Bahlahlobisisi ba Countstar Rigel ba khona ho hlahloba ts'ebetso ena e feletseng ea T / NK Cell-Mediated Cytotoxicity.

Lithuto tsa Cytotoxicity li etsoa ka ho ngola lisele tsa mofetše tse lebisitsoeng ka CFSE kapa ho li fetisetsa ka GFP.Hoechst 33342 e ka sebelisoa ho silafatsa lisele tsohle (lisele tsa T le lisele tsa hlahala).Ntle le moo, lisele tsa hlahala tse shebiloeng li ka silafatsoa ke CFSE.Propidium iodide (PI) e sebelisetsoa ho silafatsa lisele tse shoeleng (lisele tsa T le lisele tsa hlahala).Khethollo lipakeng tsa lisele tse fapaneng e ka fumanoa ho sebelisoa leano lena la ho silafatsa.

GFP Transfection Effective, Liphatseng tsa lefutso tsa limolek'hule, mefuta e fapaneng ea lintho tse phelang, le biology ea lisele, mofuta oa GFP o sebelisoa khafetsa joalo ka motlalehi oa lithuto tsa polelo.Hajoale, bo-ramahlale ba tloaetse ho sebelisa li-microscopes tsa fluorescent kapa li-cytometer tsa phallo ho lekola bokhoni ba phetisetso ea lisele tse anyesang.Empa ho sebetsana le theknoloji e rarahaneng ea cytometer ea phallo e tsoetseng pele ho hloka motho ea nang le boiphihlelo le ea tšoanelehang haholo.Countstar Rigel e thusa basebelisi ho etsa tlhahlobo ea ts'ebetso ea phetisetso habonolo le ka nepo ntle le litšenyehelo tsa ts'ebetso le tlhokomelo tse amanang le cytometry ea setso.

Cell Apoptosis, Tsoelo-pele ea apoptosis ea lisele e ka hlahlojoa ho sebelisoa FITC conjugated Annexin-V hammoho le 7-ADD.Masala a Phosphatidylserine (PS) hangata a fumaneha ka lehlakoreng le ka hare la lera la plasma la lisele tse phetseng hantle.Nakong ea apoptosis ea pele, botšepehi ba membrane boa lahleha 'me PS e tla fetisetsoa ka ntle ho lera la sele.Annexin V e na le kamano e matla le PS kahoo ke letšoao le loketseng bakeng sa lisele tsa pele tsa apoptotic.

Cell Cycle, Nakong ea karohano ea lisele, lisele li na le palo e eketsehileng ea DNA.E ngotsoe ke PI, keketseho ea matla a fluorescence e lekana ka ho toba le ho bokella DNA.Phapang ea matla a fluorescence ea lisele tse le 'ngoe ke matšoao a boemo ba sebele ba potoloho ea lisele tsa MCF 7 li ne li tšoaroa ka 4μM ea Nocodazole ho tšoara lisele tsena ka mekhahlelo e fapaneng ea potoloho ea lisele tsa bona.Litšoantšo tsa lebala le khanyang tse fumanoeng nakong ea teko ena li re lumella ho khetholla sele e 'ngoe le e' ngoe.Mocha oa PI fluorescence oa Countstar Rigel o tsebahatsa matšoao a DNA a lisele tse le 'ngoe esita le ka kakaretso.Tlhahlobo e qaqileng ea matla a fluorescence e ka etsoa ho sebelisoa FCS.

CD Marker Phenotyping, Mehlala ea Countstar Rigel e fana ka mokhoa o potlakileng, o bonolo le o bonolo haholoanyane ho, phenotyping e thehiloeng ho lisele tsa lisele tse sebetsang hantle haholoanyane.Ka litšoantšo tse nang le liqeto tse phahameng le bokhoni bo matla ba ho hlahloba lintlha, Countstar Rigel e lumella basebedisi ho fumana liphello tse tšepahalang ntle le tlhokahalo ea litlhophiso tse rarahaneng tsa taolo le liphetoho tsa matšeliso a fluorescence.

Phapang ea lisele tsa Cytokine Induced Killer (CIK) e bonts'a boleng bo ikhethang ba ts'ebetso ea Countstar Rigel analyzer ha e bapisoa ka ho toba le li-cytometer tsa phallo ea sehlopha se phahameng.Li-PBMC tsa mouse moetlong li ne li silafalitsoe ke CD3-FITC, CD4-PE, CD8-PE, le CD56-PE, 'me li susumelitsoe ke Interleukin (IL) 6. Eaba li hlahlojoa ka nako e le 'ngoe le Countstar® Rigel le Flow Cytometry.Tekong ena, CD3-CD4 , CD3-CD8, le CD3-CD56 li arotsoe ka lihlopha tse tharo, ho fumana karolo ea likarolo tse fapaneng tsa lisele.

Ho fumanoa ha Lisele tse Senyehileng ka Immunofluorescence, Li-antibodies tsa Monoclonal tse hlahisang lisele tsa lisele li tla lahleheloa ke li-clone tse ntle nakong ea ho ata ha lisele le ho feta ka lebaka la ho senyeha kapa liphetoho tsa lefutso.Tahlehelo e phahameng e tla ama haholo tlhahiso ea ts'ebetso ea tlhahiso.Tlhokomelo ea ho senyeha e phetha karolo ea bohlokoa taolong ea ts'ebetso ho fetisetsa chai ea li-antibodies ho ea holimo.

Bongata ba li-antibodies tse entsoeng indastering ea BioPharma li ka bonoa ka ho ngola li-immunofluorescence le ho hlahlojoa ka bongata ke letoto la Countstar Rigel.Litšoantšo tse khanyang le tsa fluorescence tse ka tlase li bontša ka ho hlaka li-clone tse ileng tsa lahleheloa ke tšobotsi ea tsona ho hlahisa li-antibodies tse lakatsehang.Tlhahlobo e qaqileng haholoanyane le software ea DeNovo FCS Express Image e tiisa hore 86.35% ea lisele tsohle li hlahisa li-immunoglobulins, ke 3.34% feela e leng mpe ka ho hlaka.

Trypan (capitalize B in Blue) Palo ea Cell, Trypan blue staining e ntse e sebelisoa boholo ba li-labs tsa setso sa lisele.

Trypan Blue Viability le Cell Density BioApp e ka kenngoa mefuteng eohle ea Countstar Rigel.Li-algorithms tsa rona tse sirelelitsoeng tsa ho lemoha litšoantšo li sekaseka liparamente tse fetang 20 ho hlophisa ntho ka 'ngoe e fumanoeng.

Cell Line Storage QC, Polokelong ea lisele, mohopolo o tsoetseng pele oa taolo ea boleng o netefatsa ts'ebetso e bolokehileng, e sebetsang hantle ea lihlahisoa tsohle tsa cellular.Sena se tiisa boleng bo tsitsitseng ba cell cryopreserved, cryo-served bakeng sa liteko, nts'etsopele ea ts'ebetso, le tlhahiso.

Countstar Rigel e fumana litšoantšo tse nang le liqeto tse phahameng, e sekaseka litšobotsi tse fapaneng tsa morphological tsa lintho tsa cellular joalo ka bophara, sebopeho le tloaelo ea ho kopanya.Litšoantšo tsa mehato e fapaneng ea ts'ebetso li ka bapisoa habonolo le tse ling.Kahoo liphapang tsa sebopeho le ho bokellana li ka bonoa habonolo, ka ho qoba litekanyo tsa motho.'Me database ea Countstar Rigel e na le tsamaiso e rarahaneng ea ho boloka le ho fumana litšoantšo le data.