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Countstar BioFerm

Countstar automated fungus suspension cell analyzer

The Countstar BioFerm automated fungi cell analyzer e kopanya mekhoa ea khale ea ho silafatsa e sebelisa Methylene Blue, Trypan Blue, Methylene Violet, kapa Erythrosin B e nang le litšoantšo tse phahameng.Mekhoa e tsoetseng pele ea ho lemoha litšoantšo e fana ka tlhahlobo e nepahetseng le e nepahetseng ea lisele tsa li-fungus tse sebetsang le tse shoeleng, mahloriso a lisele tsa tsona, bophara le tlhahisoleseding e mabapi le morphology le.Sistimi e matla ea taolo ea data e boloka sephetho le litšoantšo ka botshepehi mme e lumella tlhahlobo e ncha ka nako efe kapa efe.  

 

Sebaka sa Kopo

Countstar BioFerm e khona ho bala le ho hlahloba mefuta e mengata ea li-fungus (le likarolo tsa eona) ka bophara bo pakeng tsa 2μm ho isa ho 180μm.Lefapheng la biofuel le biopharma, Countstar BioFerm e pakile bokhoni ba eona e le sesebelisoa se tšepahalang le se potlakileng sa ho beha leihlo lits'ebetso tsa tlhahiso.

 

Melemo ea Basebelisi

  • Boitsebiso bo bongata ka li-fungus
    Lintlha li kenyelletsa lintlha tse mabapi le ho tsepamisa maikutlo, ho sebetsa, bophara, compactness, le sekhahla sa ho kopanya.
  • Theknoloji ea rona e nang le patented "Fixed Focus Technology"
    Ha ho na tlhoko ka nako efe kapa efe ea ho lokisa tsepamiso ea Countstar BioFerm.
  • Benche ea optical e nang le kh'amera ea mebala ea 5-megapixel
    E netefatsa pono e nang le phapang e ngata le e qaqileng ea lintho tse phelang.
  • Mojule oa tlhahlobo ea Aggregation
    E lumella polelo e tšepahalang mabapi le ts'ebetso ea budding
  • Lisebelisoa tse baballang litšenyehelo
    Maemo a mahlano a mehlala ho Countstar Chamber Slide e le 'ngoe a fokotsa litšenyehelo tsa ho sebetsa, litšila tsa polasetiki, 'me a boloka nako ea liteko.
  • Lintlha tsa sehlahisoa
  • Litlhaloso tsa Tekheniki
  • Khoasolla
Lintlha tsa sehlahisoa

 

 

Mehlala ea Litšoantšo tsa Baker's Yeast Saccharomyces cerevisiae

 

Litšoantšo tsa tomoso ea baapehi Saccharomyces cerevisiae e fumanoe ka Countstar BioFerm. Mehlala e nkiloe lits'ebetsong tse fapaneng tsa tlhahiso, karolo e 'ngoe e na le Methylene Blue (ka tlase ka letsohong le letšehali) le Methylene Violet (ka tlase ho le letona)

 

 

 

Saccharomyces cerevisiae ka methati e fapaneng ea mohato oa 2-step fermentation

 

Ka holimo ka ho le letšehali: Karolo ea setšoantšo sa Countstar BioFerm se bontšang setso sa ho qala, se silafalitsoeng ke Methylene Blue (MB).Mohlala o na le lisele tse ngata tsa lisele le lisele li sebetsa haholo (ho shoa ho lekanyelitsoeng <5%).Ka tlaase ka ho le letšehali: sampuli e sa hloekang ho tsoa ho bioreactor e sa tsoa kenngoa;li-buds li bonahala ka ho hlaka.Ka ho le letona ka tlaase: Mohlala o nkiloeng mothating oa ho qetela oa ho belisoa, o silafalitsoe ke 1:1 ka MB (mafu a lekantsoeng: 25%).Metsu e khubelu e tšoaea lisele tse shoeleng, tse kenyelletsang dae ea MB, e lebisang ho 'mala o lefifi oa volumen eohle ea sele.

 

 

 

Ho bapisa lintlha tsa tekanyo

 

Litšoantšo tse ka holimo li bonts'a papiso ea Countstar BioFerm le ho bala ka letsoho, le phapang e kholo e tlase liphethong tsa tekanyo, ha e bapisoa le lipalo tsa hemocytometer.

 

Papiso ea tlhahlobo ea kabo ea bophara ea matsoho le e iketsang

 

 

Lits'oants'o tse kaholimo li bonts'a boemo bo holimo ba litekanyo tsa bophara ba Countstar BioFerm ho tlhahlobo ea matsoho ho hemocytometer.Joalo ka ha bukana e bala palo e tlase ea lisele tse 100 e hlahlojoa, mokhoa oa kabo ea bophara o fapana haholo ho feta ho Countstar BioFerm, moo lisele tsa tomoso tse ka bang 3,000 li ileng tsa hlahlojoa.

 

 

 

Ho ikatisa ha palo ea lisele le sekhahla sa ho shoa

 

Li-aliquots tse 25 tsa diluted Saccharomyces cerevisiae disampole, tse nang le palo e akaretsang ea 6.6 × 106 liseleng / mL li ile tsa hlahlobjoa ka tsela e tšoanang ke Countstar BioFerm le ka hemocytometer ka letsoho.

Lits'oants'o ka bobeli li bonts'a phapang e phahameng haholo lipalong tsa sele e le 'ngoe, e etsoang ka letsoho ho hemocytometer.Ka lehlakoreng le leng, Countstar BioFerm e fapana hanyane ho tloha ho boleng bo ikhethileng ba mahloriso (ka ho le letšehali) le lefu (ka ho le letona).

 

Saccharomyces cerevisiae ka methati e fapaneng ea mohato oa 2-step fermentation

 

Saccharomyces cerevisiae, e silafalitsoe ke Methylene Violet mme hamorao ea hlahlobisisoa ka Countstar Sistimi ea BioFerm

Ka ho le letšehali: Karolo ea setšoantšo se fumanoeng sa Countstar Bioferm Ka ho le letona: Karolo e tšoanang, lisele tse ngotsoeng ke Countstar Li-algorithms tsa ho lemoha litšoantšo tsa BioFerm.Lisele tse sebetsang li pota-potiloe ke li-circles tse tala, lisele tse silafetseng (tse shoeleng). e tšoailoeng ka selikalikoe se mosehla (ho feta moo e bonts'itsoeng bukana ena e nang le metsu e mosehla).E kopanetsoe lisele li pota-potiloe ke li-circles tse pinki.Palo e phahameng ea li-aggregate tse peli tsa lisele li bonahala - pontšo e hlakileng ea ts'ebetso ea budding ea setso sena, Metsu e Yellow, e kenngoa ka letsoho, e tšoaea lisele tse shoeleng.

 

Kakaretso ea histogram ea ho belisoa ha tomoso e ntseng e hola ka mokhoa o hlakileng e fana ka maikutlo a boemo bo phahameng ba ts'ebetso e ntseng e tsoela pele, e bonts'ang haholo likarolo tse 2 tsa lisele,

Litlhaloso tsa Tekheniki

 

 

Litlhaloso tsa Tekheniki
Sephetho sa Lintlha Ho tsepamisa maikutlo, ho shoa ha batho, bophara, sekhahla sa ho kopanya, ho kopana
Mekhahlelo ea Tekanyo 5.0 x 10 4 – 5.0 x 10 7 /ml
Boholo Range 2 - 180 μm
Molumo oa Phaposi 20 μl
Nako ea Tekanyo <20 metsotsoana
Sephetho Format JPEG/PDF/Excel spreadsheet
Phello 5 lisampole / Countstar Chamber Slide

 

 

Litlhaloso tsa Slide
Lintho tse bonahalang Poly-(Methyl) Methacrylate (PMMA)
Boholo: 75 mm (w) x 25 mm (d) x 1.8 mm (h)
Botebo ba Chamber: 190 ± 3 μm (feela 1.6% kheloha ka bolelele bakeng sa ho nepahala ho phahameng)
Molumo oa Phaposi 20 μl

 

 

Khoasolla
  • Bukana ea Countstar BioFerm.pdf Khoasolla
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