Kev sim raws tu qauv
Lub cytotoxicity % yog xam los ntawm qhov sib npaug hauv qab no.
Cytotoxicity % = (Siv suav ntawm kev tswj - Nyob suav ntawm kev kho) / Nyob suav ntawm kev tswj × 100
Los ntawm daim ntawv lo rau lub hom phiaj qog hlwb nrog cov tsis muaj tshuaj lom, tsis muaj hluav taws xob calcein AM lossis kis nrog GFP, peb tuaj yeem saib xyuas kev tua cov qog hlwb los ntawm CAR-T hlwb.Thaum cov kab mob qog noj ntshav nyob hauv lub hom phiaj yuav raug sau los ntawm ntsuab calcein AM lossis GFP, cov hlwb tuag tsis tuaj yeem khaws cov xim ntsuab.Hoechst 33342 yog siv rau stain tag nrho cov hlwb (ob leeg T hlwb thiab qog hlwb), lwm yam, lub hom phiaj qog hlwb tuaj yeem stained nrog membrane khi calcein AM, PI yog siv rau stain lub hlwb tuag (ob leeg T hlwb thiab qog hlwb).Lub tswv yim staining no tso cai rau kev ntxub ntxaug ntawm cov hlwb sib txawv.
E: T Ratio nyob ntawm Cytotoxicity ntawm K562
Piv txwv Hoechst 33342, CFSE, PI fluorescent duab yog K562 lub hom phiaj ntawm t = 3 teev
Cov duab fluorescent tshwm sim pom qhov nce hauv Hoechst + CFSE + PI + Lub hom phiaj hlwb li E: T piv tau nce
