Habka tijaabada ah
Sitotoxicity% waxaa lagu xisaabiyaa isla'egta hoose.
Cytotoxicity% = (Tirooyinka kontoroolka nool - tirooyinka nool ee la daweeyay) / Tirooyinka kontoroolka tooska ah × 100
Marka lagu calaamadiyo unugyada burada bartilmaameedka leh calcein AM aan sun ahayn, ama aan ku gudbin GFP, waxaan la socon karnaa dilka unugyada burooyinka ee unugyada CAR-T.Halka unugyada kansarka ee la beegsanayo lagu calaamadin doono calcein cagaaran AM ama GFP, unugyada dhintay ma sii haysan karaan dheeha cagaaran.Hoechst 33342 waxaa loo isticmaalaa wasakhda dhammaan unugyada (labadaba unugyada T iyo unugyada burooyinka), haddii kale, unugyada burooyinka bartilmaameedka ah waxaa lagu dhejin karaa calcein AM, PI waxaa loo isticmaalaa wasakhaynta unugyada dhintay (labadaba unugyada T iyo unugyada burooyinka).Istaraatiijiyadan wasakhaynta ayaa u oggolaanaysa in la takooro unugyo kala duwan.
E: T Saamiga ku-tiirsanaanta Cytotoxicity ee K562
Tusaalaha Hoechst 33342, CFSE, PI sawirada fluorescent waa unugyada bartilmaameedka K562 ee t = 3 saacadood.
Sawirada fluorescent ee ka soo baxay waxay muujiyeen kororka Hoechst + CFSE + PI + unugyada Target sida E: saamiga T uu kordhay.
