Dual-fluorescence Viability (AO/PI), Acridine orange (AO) iyo propidium iodide (PI) waa midabaynta nukliyeerka iyo midabaynta aashitada.AO waxay geli kartaa xuubka unugyada dhintay iyo kuwa noolba waxayna wasakhaysaa xudunta, taasoo dhalinaysa fluorescence cagaaran.Taas bedelkeeda, PI waxa ay kaliya dhex geli kartaa xuubabka burburay ee unugyo nukleed dhintay, iyaga oo dhalinaya fluorescence cas.Tignoolajiyada sawirka ku salaysan ee Countstar Rigel waxay ka reebaysaa jajabyada unugyada, qashinka, iyo walxaha artifact iyo sidoo kale dhacdooyinka aan la qiyaasi karin sida platelet, taasoo siinaysa natiijo sax ah.Gabagabadii, nidaamka Countstar Rigel waxaa loo isticmaali karaa tallaabo kasta oo ka mid ah habka wax soo saarka unugyada.

Cytotoxicity-Cytotoxicity-Cell- Dhexdhexaadin T/NK, Daawaynta unugyada CAR-T ee dhawaan FDA-ansixisay, T-lymphocytes hidde ahaan loo farsameeyay waxay si gaar ah ugu xidhaan unugyada kansarka ee la beegsaday (T) wayna dilaan.Falanqeeyayaasha Countstar Rigel ayaa awood u leh inay falanqeeyaan nidaamkan dhamaystiran ee Cytotoxicity-ka Dhex-dhexaadinta T/NK.

Daraasadaha cytotoxicity waxaa lagu sameeyaa iyada oo lagu calaamadiyo unugyada kansarka bartilmaameedka leh CFSE ama lagu beddelo GFP.Hoechst 33342 waxaa loo isticmaali karaa in lagu wasakheeyo dhammaan unugyada (labadaba unugyada T iyo unugyada burooyinka).Haddii kale, unugyada burooyinka ee bartilmaameedka ah waxaa lagu sumayn karaa CFSE.Propidium iodide (PI) waxaa loo isticmaalaa in lagu wasakheeyo unugyada dhintay (labadaba unugyada T iyo unugyada burooyinka).Takoorka u dhexeeya unugyo kala duwan ayaa lagu heli karaa iyadoo la adeegsanayo istaraatiijiyadan wasakheynta ah.

Wax ku oolnimada Gudbinta GFP, hidde-sideyaasha molecular, noole moodeel oo kala duwan, iyo bayoolaji unug, hidde-sidaha GFP waxaa had iyo jeer loo adeegsadaa wariye ahaan daraasaadka muujinta.Hadda, saynisyahannadu waxay caadi ahaan isticmaalayaan mikroskoobyada fluorescent ama cytometers socodka si ay u falanqeeyaan waxtarka gudbinta unugyada naasleyda.Laakin maaraynta tignoolajiyada kakan ee cytometer socodka horumaray waxay u baahantahay hawlwadeen khibrad iyo aqoon sare leh.Countstar Rigel waxa ay u sahlaysa isticmaalayaasha in ay si fudud oo sax ah u gutaan qiimaynta hufnaanta isu gudbinta iyada oo aan la helin hawlgalka iyo kharashaadka dayactirka ee la xidhiidha cytometry socodka dhaqameed.

Unugyada Apoptosis-ka, Horumarka apoptosis-ka unugyada waxaa lagula socon karaa iyadoo la isticmaalayo FITC isku-dhafan Annexin-V oo lagu daray 7-ADD.Hadhaaga Phosphatidylserine (PS) waxay caadi ahaan ku yaalliin dhinaca gudaha ee xuubka balaasmaha ee unugyada caafimaadka qaba.Xilliga hore ee apoptosis, daacadnimada xuubku way lumi doontaa PS waxa loo wareejin doonaa bannaanka xuubka unugyada.Annexin V waxay xidhiidh xooggan la leedahay PS sidaas darteed waa calaamadda ugu habboon ee unugyada apoptotic-ka hore.

Wareegga unugga, Inta lagu jiro kala qaybinta unugyada, unugyadu waxay ka kooban yihiin xaddi badan oo DNA ah.Waxaa calaamad u ah PI, kororka xoogga florescence wuxuu si toos ah ula siman yahay ururinta DNA-da.Farqiga u dhexeeya xoojinta fluorescence ee unugyada kelida ah ayaa ah tilmaamayaasha xaaladda dhabta ah ee wareegga unugyada MCF 7 unugyada waxaa lagu daaweeyay 4μM ee Nocodazole si loo xiro unugyadaas marxalado kala duwan ee wareegga unugyada.Sawirrada bannaanka ifaya ee la helay intii lagu jiray xaaladda tijaabadan waxay noo oggolaanaysaa inaan aqoonsanno unug kasta.Kanaalka PI fluorescence ee Countstar Rigel wuxuu tilmaamayaa calaamadaha DNA-da ee unugyada hal unug xitaa marka la isku daro.Falanqayn faahfaahsan oo ku saabsan xoojinta fluorescence ayaa la samayn karaa iyadoo la adeegsanayo FCS.

CD Marker Phenotyping, Moodooyinka Countstar Rigel waxay bixiyaan hab degdeg ah, fudud oo xasaasi ah oo ku saabsan, fenotyping-ku-saleysan difaaca unugyada si hufan.Iyada oo la adeegsanayo sawirada xallinta sare iyo awoodaha falanqaynta xogta isku dhafan ee xoogga leh, Countstar Rigel waxay u ogolaataa dadka isticmaala inay gaaraan natiijooyin joogto ah oo la isku halleyn karo iyada oo aan loo baahnayn goobo kontarool oo adag oo ballaaran iyo hagaajinta magdhowga fluorescence.

Kala soocida unugyada Cytokine Induced Killer (CIK) waxay tusinaysaa tayada wax qabad ee aadka u wanaagsan ee falanqeeyaha Countstar Rigel marka la barbar dhigo cytometer socodka heerka sare.PBMC-yada jiirka ee dhaqanka waxaa lagu dheehday CD3-FITC, CD4-PE, CD8-PE, iyo CD56-PE, waxaana keenay Interleukin (IL) 6. Kadibna isku mar lagu falanqeeyay Countstar® Rigel iyo Flow Cytometry.Imtixaankan, CD3-CD4, CD3-CD8, iyo CD3-CD56 ayaa loo qaybiyay saddex kooxood, si loo go'aamiyo saamiga unugyada kala duwan ee unugyada.

Ogaanshaha Unugyada xumaaday ee Immunofluorescence, unugyada difaaca jirka ee Monoclonal ee soo saara xariiqyada unugyada waxay lumin doonaan qaar ka mid ah kloonyada togan inta lagu jiro kororka unugyada iyo gudbinta sababtoo ah hoos u dhaca ama isbeddellada hidde-sidaha.Khasaare sare ayaa si weyn u saameyn doona wax soo saarka habka wax soo saarka.La socodka xaalufku waxa uu door muhiim ah ka ciyaaraa xakamaynta habka si loogu wareejiyo dhalidda unugyada difaaca sida ugu wanaagsan.

Inta badan unugyada difaaca jirka ee laga soo saaray warshadaha BioPharma waxaa lagu ogaan karaa calaamadeynta difaaca jirka waxaana si qiyaas ah u falanqeeyay taxanaha Countstar Rigel.Sawirrada kanaalka ifaya iyo kanaalka ifaya ee hoos ku yaal waxay si cad u muujinayaan clones-ka lumay sifadoodii si ay u soo saaraan unugyada difaaca ee la doonayo.Falanqaynta faahfaahsan ee barnaamijka DeNovo FCS Express Image software waxay xaqiijinaysaa, in 86.35 % dhammaan unugyada ay muujinayaan immunoglobulins, kaliya 3.34% ayaa si cad u xun.

Trypan (caasimad ka dhig B ee Buluugga ah) Tirinta unugyada, midabaynta buluuga ah ee Trypan ayaa weli lagu isticmaalaa inta badan shaybaadhka dhaqanka unugga.

The Trypan Blue Viability iyo BioApp Cufnaanta Unug ayaa lagu rakibi karaa dhammaan noocyada Countstar Rigel.Algorithms-yada aqoonsiga sawirka ee la ilaaliyo waxay falanqeeyaan in ka badan 20 cabbir si loo kala saaro shay kasta oo la ogaado.

Kaydinta Khadka Unugyada QC, Kaydinta unugyada, fikradda maaraynta tayada casriga ah waxay hubisaa badbaadada, la socodka wax-ku-oolka ah ee dhammaan alaabta gacanta.Tani waxay dammaanad qaadaysaa tayada xasiloon ee unugyada cryopreserved, cryo-loo xafiday tijaabooyinka, horumarinta habka, iyo wax soo saarka.

Countstar Rigel waxa uu helaa muuqaalo heer sare ah, isaga oo falanqeynaya sifooyinka qaab-dhismeedka kala duwan ee walxaha gacanta sida dhexroorka, qaabka, iyo u janjeersiga isu-ururinta.Sawirada talaabooyinka geedi socodka kala duwan ayaa si fudud loo barbar dhigi karaa midba midka kale.Haddaba kala duwanaanshaha qaabka iyo isu geynta ayaa si fudud loo ogaan karaa, iyadoo laga fogaanayo cabbirrada bini aadamka.Xogta Countstar Rigel waxay leedahay nidaam maarayn oo casri ah oo loogu talagalay kaydinta iyo soo celinta sawirada iyo xogta.