Dual-fluorescence Viability (AO/PI), Acridine orenji (AO) uye propidium iodide (PI) inyukireya nucleic staining uye acid-inosunga dhayi.AO inogona kupinda mukati me membrane yeese akafa uye masero mapenyu uye inosvibisa nucleus, ichigadzira yegirinhi fluorescence.Kusiyana neizvi, PI inogona kungopinda mukati memembrane inoparara yemasero akafa nucleated, ichigadzira red fluorescence.Iyo tekinoroji-yakavakirwa tekinoroji yeCountstar Rigel haisanganisi zvimedu zvemasero, marara, uye zvimedu zvekugadzira pamwe nezviitiko zvakaderera senge maplatelet, zvichipa mhedzisiro chaiyo.Mukupedzisa, iyo Countstar Rigel system inogona kushandiswa kune yega nhanho yesero kugadzira maitiro.

T/NK Cell-Mediated Cytotoxicity, Munguva pfupi yapfuura FDA-yakatenderwa CAR-T cell therapy, maT-lymphocyte akagadzirwa nemajini anosunga zvakananga kumaseru egomarara akananga (T) uye anoauraya.The Countstar Rigel analyzers vanokwanisa kuongorora iyi yakazara maitiro eT/NK Cell-Mediated Cytotoxicity.

Zvidzidzo zveCytotoxicity zvinoitwa nekunyora mazita ekunangwa masero egomarara neCFSE kana kuvatapurirana neGFP.Hoechst 33342 inogona kushandiswa kusvibisa maseru ese (ese maT masero uye bundu masero).Neimwe nzira, masero anonangwa bundu anogona kusvibiswa neCFSE.Propidium iodide (PI) inoshandiswa kusvibisa masero akafa (ese maT masero uye bundu masero).Kusarura pakati pemasero akasiyana-siyana kunogona kuwanikwa uchishandisa iyi staining strategy.

GFP Transfection Efficiency, Mu molecular genetics, zvisikwa zvakasiyana-siyana zvemuenzaniso, uye cell biology, GFP gene inowanzoshandiswa semutori wenhau pazvidzidzo zvekutaura.Parizvino, masayendisiti anowanzo kushandisa maikorosikopu efluorescent kana kuyerera kwema cytometer kuongorora kugona kwekutapurirana kwemasero emammalian.Asi kubata tekinoroji yakaoma yeyepamusoro kuyerera cytometer inoda ane ruzivo uye ane hunyanzvi mushandisi.Countstar Rigel inogonesa vashandisi kuita nyore uye nemazvo dhizaini yekufambisa pasina kushanda uye kugadzirisa mari ine chekuita neyechinyakare kuyerera cytometry.

Cell Apoptosis, Kufambira mberi kwesero apoptosis kunogona kuongororwa uchishandisa FITC conjugated Annexin-V pamwe chete ne7-ADD.Phosphatidylserine (PS) masara anowanzo kuwanikwa mukati mechikamu cheplasma membrane yemasero ane hutano.Munguva yekutanga apoptosis, iyo membrane kutendeseka inorasika uye PS inozoendeswa kunze kwesero membrane.Annexin V ine hukama hwakasimba kuPS uye nekudaro ndiyo yakanakira chiratidzo chekutanga apoptotic masero.

Cell Cycle, Panguva yekupatsanurwa kwesero, maseru ane huwandu hwakawedzerwa hweDNA.Yakanyorwa nePI, kuwedzera kwekusimba kwefluorescence kunoenderana zvakananga nekuunganidzwa kweDNA.Misiyano yekusimba kwefluorescence yemasero mamwechete ndiyo zviratidzo zvechimiro chaicho chesero cycle MCF 7 masero akabatwa ne 4μM yeNocodazole kuti asunge masero aya pamatanho akasiyana emasero avo.Iyo inopenya-yemunda mifananidzo yakawanikwa panguva iyi yekuyedza mamiriro inotibvumira kuona sero rega rega.Iyo PI fluorescence chiteshi che Countstar Rigel inotaridza masaini eDNA emasero mamwechete kunyangwe akaunganidzwa.Ongororo yakadzama ye fluorescence intensity inogona kuitwa uchishandisa FCS.

CD Marker Phenotyping, Iyo Countstar Rigel modhi inopa nzira inokurumidza, yakapusa uye inonzwisisika kune, immuno-based phenotyping yemasero zvakanyanya kushanda.Iine yakakwirira resolution mifananidzo uye ane simba akabatanidzwa ekuongorora data kugona, iyo Countstar Rigel inobvumira vashandisi kuwana zvinogara zvakavimbika mhedzisiro pasina kudiwa kweakaomesesa kudzora marongero uye fluorescence muripo wekugadzirisa.

Iyo Cytokine Induced Killer (CIK) cell differentiation inoratidza yakanakisa mashandiro emhando yeCountstar Rigel analyzer mukuenzanisa yakananga kune yakakwira class flow cytometers.PBMCs yembeva mutsika yakasvibiswa neCD3-FITC, CD4-PE, CD8-PE, uye CD56-PE, uye yakakonzerwa neInterleukin (IL) 6. Zvadaro yakaongororwa panguva imwe chete neCountstar® Rigel uye Flow Cytometry.Mubvunzo uyu, CD3-CD4 , CD3-CD8, neCD3-CD56 zvakakamurwa kuita mapoka matatu, kuti zvionekwe huwandu hwemasero akasiyana.

Kuonekwa kweMasero Akaderedzwa neImmunofluorescence, Monoclonal antibodies inogadzira maseru mitsara inorasikirwa nemamwe maclone akanaka panguva yekuwedzera kwesero uye kupfuura nekuda kwekuora kana genetic mutations.Kurasikirwa kwepamusoro kuchakanganisa zvakanyanya kugadzirwa kwemaitiro ekugadzira.Kutariswa kwekudzikira kunoita basa rakakosha mukudzora kwemaitiro kushandura goho remasoja ekudzivirira chirwere kusvika pakanyanya.

Mazhinji emasoja ekudzivirira chirwere anogadzirwa muindasitiri yeBioPharma anogona kuonekwa ne immunofluorescence label uye nekuongororwa huwandu ne Countstar Rigel series.Iyo inopenya-munda uye fluorescence chiteshi mifananidzo pazasi inoratidza zvakajeka iwo ma clones akarasa hunhu kuburitsa anodiwa masoja ekudzivirira chirwere.Ongororo yakadzama neDeNovo FCS Express Image software inosimbisa, kuti 86.35 % yemasero ese ari kuratidza ma immunoglobulins, 3.34 % chete ndiwo asina kunaka.

Trypan (capitalize B muBlue) Kuverengera Sero, Trypan blue staining ichiri kushandiswa muhuwandu hwemasero tsika marabhu.

Iyo Trypan Blue Viability uye Cell Density BioApp inogona kuiswa pane ese Countstar Rigel modhi.Yedu yakadzivirirwa yekucherechedza mufananidzo algorithms inoongorora anopfuura makumi maviri paramita kurongedza chinhu chimwe nechimwe chakaonekwa.

Cell Line Storage QC, Muchitokisi chekuchengetedza, yakaomesesa yemhando manejimendi pfungwa inochengetedza yakachengeteka, inonyatso tarisisa yeese zvigadzirwa zvema cellular.Izvi zvinovimbisa kugadzikana kwemhando ye cell cryopreserved, cryo-yakachengetedzwa kuti iedze, kuvandudza maitiro, uye kugadzira.

Iyo Countstar Rigel inowana yakakwirira-resolution mifananidzo, ichiongorora akasiyana morphological hunhu hwezvinhu zvema cellular senge dhayamita, chimiro, uye kuunganidza maitiro.Mifananidzo yematanho akasiyana-siyana anogona kuenzaniswa nyore kune mumwe nemumwe.Saka misiyano yechimiro uye kuunganidzwa inogona kuoneka zviri nyore, nekudzivisa zviyero zvevanhu.Uye iyo Countstar Rigel dhatabhesi ine yakaomesesa manejimendi system yekuchengetedza uye kudzoreredza kwemifananidzo uye data.