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Kushandisa Mufananidzo Cytometer Kuvandudza Cell Line Development yeBiologics uye rAAV Production

Biologics uye AAV-yakavakirwa gene marapirwo ari kuwana yakawanda musika mugove wekurapwa kwechirwere.Nekudaro, kugadzira yakasimba uye inoshanda mammalian cell line yekugadzira kwavo kunonetsa uye kazhinji kunoda yakakura cellular characterization.Nhoroondo, cytometer inoyerera inoshandiswa mune aya maseru-based assays.Nekudaro, cytometer inoyerera inodhura uye inosanganisira kudzidziswa kwakawanda kwese kushanda nekugadzirisa.Munguva pfupi yapfuura, nekuwedzera kwekugona kwekombuta uye yepamusoro-mhando kamera sensors, mufananidzo-yakavakirwa cytometry yakagadziridzwa kuti ipe chaiyo uye inodhura-inoshanda imwe nzira yekusimudzira mutsara mutsara.Mubasa iri, isu takatsanangura sero mutsara wekuvandudza mafambiro unosanganisira mufananidzo-based cytometer, iyo inonzi Countstar Rigel, yekutapurirana kunyatsoshanda ongororo uye yakagadzikana dziva kuongororwa uchishandisa CHO uye HEK293 masero anoratidza antibody uye rAAV vector, zvichiteerana.Muzvidzidzo zviviri izvi, takaratidza:

  1. Countstar Rigel yakapa yakafanana yekuona chokwadi kuyerera cytometry.
  2. Countstar Rigel -based pool evaluation inogona kubatsira kuona boka rinodikanwa resingle-cell cloning (SCC).
  3. Countstar Rigel yakabatanidzwa sero mutsara wekusimudzira chikuva chakawana 2.5 g/L mAb titer.

Isu takakurukura zvakare mukana wekushandisa Countstar seimwe nhanho yeRAAV DoE-yakavakirwa optimization chinangwa.

 

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