Product features
Innovative Optical Multiplication Technology
Unique zooming tekinoroji inogonesa vashandisi kuongorora maseru muhupamhi hwemadhayamita
Paunenge uchishandisa iyo inopenya munda BioApp matemplate mu Countstar Mira, iyo novel Zooming Technology inogonesa mushandisi kuona nemazvo zvinhu zvemaserura mukati medhiyamita renji kubva pa1.0µm kusvika 180.0µm.Mifananidzo yakatorwa inoratidza kunyange ruzivo rwesero rimwe chete.Izvi zvinowedzera huwandu hwemashandisirwo kunyangwe kune zvinhu zvemaserura, izvo zvaisakwanisa kuongororwa chaizvo munguva yakapfuura.
| Mienzaniso yeakajairwa maseru mitsara mukuwirirana kune inosarudzwa magnifications 5x, 6.6x, uye 8x |
| Magnification Diameter range | 5x | 6.6x | 8x |
| >10µm | 5-10 µm | 1-5 µm |
| Kuverenga | ✓ | ✓ | ✓ |
| Vability | ✓ | ✓ | ✓ |
| Sero Type | - MCF7
- HEK293
- CHO
- MSC
- RAW264.7
| - Immune Cell
- Doro mbiriso
- Zebrafish embryo cells
| - Pichia Pastoris
- Chlorella vulgaris (FACHB-8)
- Escherichia
|
Progressive AI yakavakirwa Image Analysis algorithms
Iyo Countstar Mira FL inoshandisa mabhenefiti eArtifical Intelligence kugadzira yega-yekudzidza algorithms.Vanokwanisa kuziva uye kuongorora maitiro akawanda emasero.Iko kubatanidzwa kwesero chimiro parameters kunobvumira kuongororwa kwakanyatso uye kudzokororwa kwechimiro chesero cycle uye / kana kuendesa data nezve kuwirirana pakati pekuchinja kwesero morphology, kuumbwa kwesero masumbu (aggregates, madiki saizi spheroids) uye mamiriro anokanganisa.
Kunyora mhedzisiro yeasina kurongeka akaumbwa Mesenchymal Stem Cells (MSC; 5x mangification) mutsika inowedzera.
- Denderedzwa regirinhi rinotara masero mapenyu
- Denderedzwa dzvuku rinoratidza masero akafa
- White madenderedzwa akaunganidza maseru
RAW264.7 cell line idiki uye nyore kuunganidzwa.Iyo Countstar AI algorithm inokwanisa kuona maseru ari muzvikwata uye kuverenga
- Denderedzwa regirinhi rinotara masero mapenyu
- Denderedzwa dzvuku rinoratidza masero akafa
- White madenderedzwa akaunganidza maseru
Saizi isina kuenzana yezebrafish embryonic masero (6.6X magnification
- Denderedzwa regirinhi rinotara masero mapenyu
- Denderedzwa dzvuku rinoratidza masero akafa
- White madenderedzwa akaunganidza maseru
Intuitive Graphical User Interface (GUI) Dhizaini
Iyo yakajeka yakarongeka GUI inobvumira kuita kwakanaka uye kwakagadzikana kuyedza kuita
- Raibhurari yakakura ine pre-set seti mhando uye BioApps (assay template protocol).Kungodzvanya kamwe chete paBioApp, uye bvunzo inogona kutanga.
- Iyo mushandisi-inoshamwaridzika GUI inoita kuti zvive nyore kuchinja pakati peakasiyana menyu sarudzo uye inovimbisa yakagadzika bvunzo chiitiko
- Yakajeka yakarongeka menyu modules inotsigira mushandisi mune yemazuva ese bvunzo maitiro
Sarudza iyo BioApp, isa Sample ID, uye tanga assay kumhanya
128 GB yepakati data yekuchengetedza simba, inokwana kuchengetedza approx.50,000 kuongororwa kunoguma mu Countstar (R) Mira.Kuti uwane nekukurumidza, data inodiwa inogona kusarudzwa nemhando dzakasiyana dzekutsvaga.
Chinhu chinobatsira kuchengetedza nguva, ndiyo inodzoreredzwa dilution Calculator.Iyo ichaunza iwo chaiwo mavhoriyamu e diluent uye epakutanga sero sampuli, kana iyo yekupedzisira kusangana kwemaseru uye vhoriyamu yakanangana yapinzwa.Izvi zvinoita kuti kupfuudza kwemasero kune ma subcultures avo kugadzikane.
Multiple application features
Iwo ekuongorora maficha e Countstar Mira anotsigira mushandisi mukunzwisisa shanduko ine simba mukati mesero tsika uye inobatsira kukwirisa mamiriro avo ekukura.
Iyo yepamusoro, AI yakavakirwa mufananidzo yekuziva software yeCountstar Mira inokwanisa kuendesa akawanda ma paramita.Kunze kwezvakajairwa mhedzisiro yekumisikidzwa kwesero uye chimiro chekushanda, kugovaniswa kwehukuru hwesero, kuumbwa kwemapoka emasero, kusimba kwefluorescence kwesero rimwe nerimwe, chimiro chekukura kwecurve, uye yavo yekunze morphology factor maparamita akakosha ekuongorora iwo chaiwo. mamiriro sero tsika.Iwo anogadzirwa otomatiki magirafu ekukura macurves, kugovera dhayamita uye fluorescence intensity histograms, kuongororwa kwesero rimwe mukati meaggregates uye kutsunga kwesero compactness parameter inogonesa mushandisi kunzwisisa zvirinani maitiro ane simba mukati meakaongororwa sero tsika kubva pakutanga kusvika pakupera kwemaitiro.
Histogram
Relative Fluorescence Intensity (RFI) kugovera histogram
Diameter kugovera histogram
Kukura curve
Yedza mufananidzo(s) uye Mibairo
Kukura curve diagram
Product Application
AO/PI dual fluorescence cell density uye mashandiro ekuongorora
Iyo dual-fluorescence AO/PI staining method yakavakirwa pamusimboti, wekuti ese madhayi, Acridine Orange (AO) nePropidium Iodide (PI), ari kupindirana pakati pemanucleic acids echromosome ari munucleus yesero.Nepo AO ichikwanisa kupinda mukati memembranes yenucleus chero nguva uye kusvibisa DNA, PI inogona kungopfuura iyo yakakanganiswa membrane yenucleus yesero rinofa (rakafa).Iyo yakaunganidzwa AO musero nucleus inoburitsa girini yakasvibira pakukwira kwe525nm, kana ichinakidzwa ne480nm, PI iri kutumira mwenje mutsvuku neamplitude yayo pa615nm, kana ichinakidzwa pa525nm.Iyo FRET (Foerster Resonance Energy Transfer) inovimbisa, kuti iyo yakaburitswa chiratidzo cheAO pa525nm inobatwa pamberi pePI dhayi kudzivirira kaviri mwenje emittance uye kudurura pamusoro.Iyi yakakosha dhayi musanganiswa weAO/PI inobvumira kusefa chaiko nucleus ine maseru pamberi peacaryotes senge erythrocytes.
Countstar Mira FL data yakaratidza mutsara wakanaka weiyo gradient dilution yeHEK293 maseru
GFP/RFP transfection performance analysis
Kubudirira kwekutapurirana indekisi yakakosha mukusimudzira mutsara uye optimization, muhutachiona vector tuning, uye yekutarisa goho rechigadzirwa muBiopharma process.Yakave bvunzo inogarogadzikiswa yekuona nekukurumidza nekuvimbika zvirimo mupuroteni inonangwa mukati mesero.Mune akasiyana gene therapy nzira, chishandiso chakakosha chekudzora kutapurirana zvakanaka kweinodiwa genetic modified.
Iyo Countstar Mira haingopi chaiyo uye yakarurama mhedzisiro, ichienzaniswa nekuyerera cytometry, uyezve iyo analyzer inopa mifananidzo sehumbowo hwehumbowo.Kunze kweizvi, inorerutsa zvakanyanya uye nekumhanyisa ongororo kuti ifambise budiriro yekuvandudza nekugadzira maitiro.
Mifananidzo yakatevedzana, yakawanikwa neCountstar(R) Mira, ichiratidza kuwedzera kwekutapurirana nhanho (kubva kuruboshwe kuenda kurudyi) yemasero akagadziridzwa genetically (HEK 293 cell line; kuratidza GFP muzvikamu zvakasiyana)
Mhedzisiro yezviyero zvekufananidza, yakaurayiwa neB / C CytoFLEX, ichisimbisa iyo GFP yekufambisa data data yeakagadziridzwa HEK 293 masero, akaongororwa mu Countstar Mira.
Yakasimbiswa zvakanyanya Trypan Blue viability ongororo
Iyo Trypan Blue viability rusarura assay ichiri imwe yedzakanyanya kushandiswa uye yakavimbika nzira yekuona huwandu hwe (kufa) maseru akafa mukati meyakamiswa sero tsika.Maseru anoshanda ane ekunze ekunze sero membrane chimiro anodzinga Trypan Blue kubva pakupinda membrane.Muchiitiko ichi, membrane yesero inodonha nekuda kwekufambira mberi kwekufa kwesero, Trypan Blue inogona kupfuura membrane barriers, inounganidza musero plasma uye kusvibisa sero bhuruu.Musiyano uyu wemaziso unogona kushandiswa kusiyanisa masero ekurarama asina kujeka kubva kumaseru akafa nemufananidzo wekuziva algorithms ye Countstar Mira FL.
- Mifananidzo yevatatu, Trypan Blue yakasvibiswa maseru mitsara, yakawanikwa muCountstar (R) Mira FL mune inopenya munda mode.
- Mhedzisiro ye dilution gradient yeHEK 293 yakatevedzana
