ʻO Dual-fluorescence Viability (AO / PI), Acridine alani (AO) a me propidium iodide (PI) he nukle nucleic staining a me nā mea hoʻopaʻa waikawa.Hiki iā AO ke komo i loko o ka membrane o nā sela make a me nā mea ola a hoʻopaneʻe i ka nucleus, e hoʻohua ana i ka fluorescence ʻōmaʻomaʻo.ʻO ka ʻokoʻa, hiki i ka PI ke hoʻoheheʻe wale i nā membrane disintegrating o nā cell nucleated make, e hana ana i ka fluorescence ʻulaʻula.Hoʻokaʻawale ka ʻenehana kiʻi kiʻi o ka Countstar Rigel i nā ʻāpana cell, nā ʻōpala, a me nā ʻāpana artifact a me nā hanana undersized e like me nā platelets, e hāʻawi ana i kahi hopena pololei loa.I ka hopena, hiki ke hoʻohana ʻia ka ʻōnaehana Countstar Rigel no kēlā me kēia pae o ke kaʻina hana kelepona.

T/NK Cell-Mediated Cytotoxicity, I loko o ka lāʻau lapaʻau CAR-T i ʻāpono ʻia e FDA, ua hoʻopaʻa ʻia nā T-lymphocytes genetically i nā pūnana maʻi maʻi i manaʻo ʻia (T) a pepehi iā lākou.Hiki i ka Countstar Rigel analyers ke kālailai i kēia kaʻina hana piha o T/NK Cell-Mediated Cytotoxicity.

Hana ʻia nā haʻawina Cytotoxicity ma ka hōʻailona ʻana i nā pūnaʻi maʻi maʻi i manaʻo ʻia me CFSE a i ʻole ka hoʻololi ʻana iā lākou me GFP.Hiki ke hoʻohana ʻia ʻo Hoechst 33342 no ka hoʻopaʻa ʻana i nā cell āpau (ʻo nā pūnaewele T a me nā pūnaewele tumo).ʻO kahi ʻē aʻe, hiki ke hoʻopaʻa ʻia nā cell tumor target me CFSE.Hoʻohana ʻia ka propidium iodide (PI) no ka hoʻopaʻa ʻana i nā sela make (ʻo nā pūnae T a me nā pūnaewele tumo).Hiki ke loaʻa ka hoʻokae ʻana ma waena o nā cell like ʻole me ka hoʻohana ʻana i kēia hoʻolālā kala.

ʻO ka maikaʻi o ka hoʻololi ʻana o GFP, i loko o nā genetics molecular, nā ʻano mea hoʻohālike like ʻole, a me ka biology cell, hoʻohana pinepine ʻia ka gene GFP ma ke ʻano he mea hoʻolaha no nā haʻawina hōʻike.I kēia manawa, hoʻohana maʻamau ka poʻe ʻepekema i nā microscope fluorescent a i ʻole flow cytometers e nānā i ka pono o ka hoʻololi ʻana o nā cell mammalian.Akā ʻo ka lawelawe ʻana i ka ʻenehana paʻakikī o kahi cytometer kahe holomua e koi ana i kahi mea hoʻohana ʻike a mākaukau loa.Hāʻawi ʻo Countstar Rigel i nā mea hoʻohana e hana maʻalahi a pololei hoʻi i kahi hoʻāʻo pono transfection me ka ʻole o ka hana a me nā kumukūʻai mālama e pili ana i ka cytometry kahe kuʻuna.

Cell Apoptosis, Hiki ke nānā ʻia ka holomua o ka cell apoptosis me ka hoʻohana ʻana i ka FITC conjugated Annexin-V i hui pū me 7-ADD.Loaʻa nā koena Phosphatidylserine (PS) ma ka ʻaoʻao o loko o ka membrane plasma o nā cell olakino.I ka wā apoptosis mua, nalowale ka pono o ka membrane a e unuhi ʻia ʻo PS i waho o ka membrane cell.Loaʻa i ka Annexin V kahi pilina ikaika me PS a no laila ʻo ia ka māka kūpono no nā cell apoptotic mua.

Cell Cycle, I ka wā o ka hoʻokaʻawale ʻana i ke kelepona, loaʻa i nā cell i ka nui o ka DNA.Hoʻopaʻa inoa ʻia e PI, ʻo ka hoʻonui ʻana i ka ikaika o ka fluorescence pili pono i ka hōʻiliʻili o DNA.ʻO nāʻokoʻa i ka ikaika o ka fluorescence o nā pūnaewele hoʻokahi,ʻo ia nā hōʻailona o ke kūlana maoli o ke kaʻina pūnaewele MCF 7 pūnaewele i mālamaʻia me 4μM o Nocodazole e hopu i kēia mau pūnaewele ma nāʻanuʻu likeʻole o ko lākou pōʻai pūnaewele.ʻO nā kiʻi ʻālohilohi i loaʻa i kēia hiʻohiʻona hoʻāʻo e ʻae iā mākou e ʻike i kēlā me kēia cell.ʻO ka PI fluorescence channel o ka Countstar Rigel e ʻike i nā hōʻailona DNA o nā pūnaeho hoʻokahi i loko o nā hui.Hiki ke hoʻokō ʻia kahi loiloi kikoʻī o ka ikaika fluorescence me ka hoʻohana ʻana i ka FCS.

ʻO CD Marker Phenotyping, ʻO nā hiʻohiʻona Countstar Rigel ke hāʻawi aku i kahi ala wikiwiki, maʻalahi a maʻalahi hoʻi i ka phenotyping ma muli o ka immunotyping o nā cell i ʻoi aku ka maikaʻi.Me nā kiʻi hoʻonā kiʻekiʻe a me nā mana hoʻopaʻa ʻikepili hoʻohui ikaika, hiki i ka Countstar Rigel i nā mea hoʻohana ke hoʻokō i nā hopena hilinaʻi mau me ka ʻole o ka pono o nā hoʻonohonoho mana paʻakikī a me nā hoʻoponopono uku fluorescence.

Hōʻike ka ʻokoʻa cell Cytokine Induced Killer (CIK) i ka maikaʻi o ka hana maikaʻi o ka Countstar Rigel analyzer i ka hoʻohālikelike pololei ʻana i nā cytometers kahe o ka papa kiʻekiʻe.ʻO nā PBMC o ka ʻiole ma ka moʻomeheu i hoʻopaʻa ʻia me CD3-FITC, CD4-PE, CD8-PE, a me CD56-PE, a hoʻoulu ʻia e Interleukin (IL) 6. A laila nānā ʻia i ka manawa like me Countstar® Rigel a me Flow Cytometry.Ma kēia ho'āʻo, ua māhele ʻia ka CD3-CD4 , ka CD3-CD8, a me ka CD3-CD56 i ʻekolu pūʻulu, e hoʻoholo ai i ka hapa o nā subpopulations cell like ʻole.

ʻO ka ʻike ʻana i nā Pūnaewele Degenerated e ka Immunofluorescence, ʻo nā antibodies Monoclonal e hana ana i nā laina kelepona e nalowale kekahi mau clones maikaʻi i ka wā o ka hoʻonui ʻana o ka cell a me ka hele ʻana ma muli o ka degradation a i ʻole nā ​​genetic mutations.ʻO kahi poho kiʻekiʻe e hoʻopilikia nui i ka huahana o ka hana hana.He kuleana koʻikoʻi ka nānā ʻana i ka hoʻohaʻahaʻa ʻana i ke kaʻina hana e hoʻololi i ka hua o nā antibodies i ka mea maikaʻi loa.

ʻO ka hapa nui o nā antibodies i hana ʻia i ka ʻoihana BioPharma hiki ke ʻike ʻia e ka immunofluorescence labeling a nānā ʻia me ka nui e ka Countstar Rigel series.Hōʻike maopopo ʻia nā kiʻi ʻālohilohi a me ka fluorescence channel ma lalo i kēlā mau clones i nalowale i ko lākou ʻano e hana ai i nā antibodies i makemake ʻia.ʻO ka ʻike kikoʻī hou aʻe me ka polokalamu DeNovo FCS Express Image hōʻoia, ʻo 86.35 % o nā cell āpau e hōʻike ana i nā immunoglobulins, ʻo 3.34% wale nō ka maikaʻi ʻole.

ʻO Trypan (ka poʻo nui B ma ka ʻulaʻula) helu kelepona, hoʻohana mau ʻia ka ʻili polū Trypan i ka hapa nui o nā keʻena moʻomeheu cell.

Hiki ke hoʻokomo ʻia ka Trypan Blue Viability a me Cell Density BioApp ma nā hiʻohiʻona Countstar Rigel āpau.Kākoʻo kā mākou algorithms ʻike kiʻi pale ʻia ma mua o 20 mau ʻāpana e hoʻokaʻawale i kēlā me kēia mea i ʻike ʻia.

Cell Line Storage QC, I loko o ka mālama ʻana i ke kelepona, kahi manaʻo hoʻokele waiwai paʻakikī e hōʻoia i ka mālama pono ʻana i nā huahana kelepona āpau.Mālama kēia i ka maikaʻi paʻa o ka cell cryopreserved, cryo-preserved no nā hoʻokolohua, hoʻomohala kaʻina hana, a me ka hana ʻana.

Loaʻa i ka Countstar Rigel nā kiʻi hoʻonā kiʻekiʻe, ke kālailai ʻana i nā hiʻohiʻona morphological like ʻole o nā mea kelepona e like me ke anawaena, ke ʻano, a me ke ʻano hoʻohui.Hiki ke hoʻohālikelike ʻia nā kiʻi o nā ʻanuʻu kaʻina hana like ʻole.No laila hiki ke ʻike maʻalahi i nā ʻano like ʻole a me ka hōʻuluʻulu ʻana, ma ka pale ʻana i nā ana kanaka.A he ʻōnaehana hoʻokele koʻikoʻi ko ka Countstar Rigel no ka mālama ʻana a me ka hoʻihoʻi ʻana i nā kiʻi a me nā ʻikepili.