Kugwiritsa Ntchito Zithunzi za Cytometer Kupititsa patsogolo Chitukuko cha Ma cell Line kwa Biologics ndi rAAV Production

Biologics ndi AAV-based gene therapy akupeza gawo lalikulu pamsika wazochiza matenda.Komabe, kupanga chingwe champhamvu komanso chogwira mtima cha ma cell a mammalian kuti apange ndizovuta ndipo nthawi zambiri kumafuna mawonekedwe amtundu wambiri.M'mbiri, cytometer yothamanga imagwiritsidwa ntchito poyesa ma cell awa.Komabe, cytometer yothamanga ndiyokwera mtengo kwambiri ndipo imaphatikizapo maphunziro ochulukirapo pakugwira ntchito ndi kukonza.Posachedwapa, ndi kuwonjezeka kwa luso la makompyuta ndi makamera apamwamba kwambiri a makamera, cytometry yochokera pazithunzi yapangidwa kuti ipereke njira yolondola komanso yotsika mtengo yopangira chitukuko cha ma cell.Mu ntchitoyi, tidalongosola kayendedwe kakapangidwe ka ma cell ophatikiza ma cytometer ozikidwa pazithunzi, omwe ndi Countstar Rigel, pakuwunika kwa magwiridwe antchito ndikuwunika kwamadzi okhazikika pogwiritsa ntchito ma cell a CHO ndi HEK293 omwe akuwonetsa antibody ndi rAAV vector, motsatana.M'maphunziro awiriwa, tidawonetsa:

1. Countstar Rigel anapereka kulondola kofananako kwa cytometry.
2. Kuwunika kwa dziwe kwa Countstar Rigel kungathandize kudziwa gulu lofunikira la single-cell cloning (SCC).
3. Countstar Rigel yophatikizira nsanja yotukula ma cell idakwanitsa 2.5 g/L mAb titer.

Tidakambirananso za kuthekera kogwiritsa ntchito Countstar ngati gawo lina la rAAV DoE-based optimization target.

kuti mumve zambiri, chonde tsitsani fayilo ya PDF.