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Aiwatar da Cytometer Hoto don Inganta Ci gaban Layin Halitta don Ilimin Halittu da Samar da rAAV

Ilimin halittu da tushen jiyya na AAV suna samun ƙarin kaso na kasuwa don maganin cututtuka.Koyaya, haɓaka layin tantanin halitta mai ƙarfi da inganci don samar da su yana da ƙalubale kuma yawanci yana buƙatar ƙirar salon salula mai faɗi.A tarihance, ana amfani da sitometer mai gudana a cikin waɗannan ƙididdigar tushen tantanin halitta.Koyaya, sitometer mai gudana yana da ɗan tsada kuma ya ƙunshi horo mai yawa don duka aiki da kulawa.Kwanan nan, tare da haɓaka ƙarfin kwamfuta da na'urori masu auna kyamarorin kyamarorin kyamarorin, an ƙirƙira sitometry na tushen hoto don samar da madaidaicin madaidaicin farashi mai inganci don haɓaka aikin layin salula.A cikin wannan aikin, mun bayyana aikin ci gaban layin salula wanda ya haɗa da cytometer na tushen hoto, wato Countstar Rigel, don kimanta ingancin canjin yanayi da kwanciyar hankali ta hanyar amfani da ƙwayoyin CHO da HEK293 waɗanda ke bayyana antibody da vector rAAV, bi da bi.A cikin nau'i biyu na binciken, mun nuna:

  1. Countstar Rigel ya ba da daidaiton ganowa iri ɗaya don kwararar sitometry.
  2. Countstar Rigel-tushen kimantawa na tafkin zai iya taimakawa wajen tantance ƙungiyar da ake so don cloning-cell (SCC).
  3. Countstar Rigel ya haɗa dandamalin haɓaka layin salula ya sami 2.5 g/L mAb titer.

Mun kuma tattauna yuwuwar amfani da Countstar azaman wani Layer na tushen ingantawa na tushen rAAV DoE.

 

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  • Aiwatar da Cytometer Hoto don Inganta Ci gaban Layin Tantanin halitta don Ilimin Halittu da Samar da rAAV.pdf Zazzagewa
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