Ke loaʻa nei nā lāʻau lapaʻau ʻo Biologics a me AAV-based gene therapies.Eia nō naʻe, paʻakikī ka hoʻomohala ʻana i kahi laina kelepona mammalian ikaika a maikaʻi no kā lākou hana ʻana a koi pinepine ʻia ka ʻike kikoʻī cellular.ʻO ka mōʻaukala, hoʻohana ʻia kahi cytometer kahe i kēia mau hoʻāʻo e pili ana i ke kelepona.Eia nō naʻe, ʻoi aku ka pipiʻi o kahi cytometer kahe a komo i ka hoʻomaʻamaʻa nui no ka hana a me ka mālama ʻana.I kēia mau lā, me ka hoʻonui ʻana i nā mana helu helu a me nā mea ʻike kiʻi kiʻekiʻe kiʻekiʻe, ua hoʻokumu ʻia ka cytometry e pili ana i ke kiʻi e hāʻawi i kahi koho kūpono a me ke kumu kūʻai no ka hoʻomohala ʻana i ke kaʻina hana kelepona.Ma kēia hana, ua wehewehe mākou i kahi kaʻina hana hoʻomohala laina kelepona e hoʻopili ana i kahi cytometer e pili ana i ke kiʻi, ʻo ia hoʻi ʻo Countstar Rigel, no ka loiloi pono o ka transfection a me ka loiloi wai paʻa me ka hoʻohana ʻana i nā cell CHO a me HEK293 e hōʻike ana i ka antibody a me rAAV vector, kēlā me kēia.Ma nā haʻawina ʻelua, ua hōʻike mākou:
- Hāʻawi ʻo Countstar Rigel i ka pololei ʻike like me ka kahe cytometry.
- Hiki ke kōkua i ka loiloi loko i hoʻokumu ʻia ʻo Countstar Rigel e hoʻoholo i ka pūʻulu i makemake ʻia no ka cloning cell single (SCC).
- Ua hoʻokomo ʻo Countstar Rigel i ka paepae hoʻomohala laina kelepona i loaʻa i ka 2.5 g/L mAb titer.
Ua kūkākūkā pū mākou i ka hiki ke hoʻohana i ka Countstar ma ke ʻano he papa ʻē aʻe o kahi rAAV DoE-based optimization target.
no ka ʻike hou aku, e ʻoluʻolu e hoʻoiho i ka faila PDF.
