I-AOPI Ubalo lwe-Dual-fluoresces luhlobo lovavanyo olusetyenziselwa ukufumanisa ukugxila kweeseli kunye nokusebenza.Isisombululo yindibaniselwano ye-acridine orange (ibala eliluhlaza-fluorescent nucleic acid) kunye nepropidium iodide (i-red-fluorescent nucleic acid stain).I-Propidium iodide (PI) yidayi yokukhutshwa kwe-membrane engena kuphela kwiiseli ezineembrane eziphazamisekileyo, ngelixa i-acridine orange iyakwazi ukungena kuzo zonke iiseli kubemi.Xa zombini iidayi zikhona kwi-nucleus, i-propidium iodide ibangela ukunciphisa i-acridine orange fluorescence nge-fluorescence resonance energy transfer (FRET).Ngenxa yoko, iiseli ze-nucleated ezine-membrane ezingaguqukiyo zingcolisa i-fluorescent eluhlaza kwaye zibalwa njengeziphilayo, kanti iiseli ze-nucleated ezinemibhobho ephazamisekileyo kuphela zibala umbala obomvu we-fluorescent kwaye zibalwa njengezifileyo xa usebenzisa inkqubo ye-Countstar® FL.Izinto ezingenayo i-nucleated ezifana neeseli ezibomvu zegazi, iiplatelet kunye ne-debris azikhuli kwaye azihoywa yi-software ye-Countstar® FL.
Inkqubo yoNyango lweStem Cell
Umzobo we-4 Ukubeka iliso ekusebenzeni kunye nokubala kweeseli ze-mesenchymal stem cells (MSCs) zokusetyenziswa kwiiseli zonyango.
Qinisekisa ukusebenza kwe-MSC nge-AO/PI kunye ne-Trypan Blue assay
Umzobo 2. A. Umfanekiso we-MSC odyojwe yi-AO/PI kunye ne-Trypan Blue;2. Ukuthelekiswa kwe-AO/PI kunye nesiphumo se-Trypan eluhlaza okwesibhakabhaka phambi nasemva kothutho.
Isalathiso se-cell refractive index, i-Trypan Blue staining yayingeyona nto icacileyo, kunzima ukumisela ukusebenza emva kokuthutha.Ngelixa i-fluorescence enemibala emibini ivumela ukungcoliswa kweeseli ze-nucleated eziphilayo nezifileyo, ivelisa iziphumo ezichanekileyo ezisebenzayo nokuba kukho inkunkuma, iiplatelets kunye neeseli ezibomvu zegazi.
