AOPI Dual-fluoresces ƙidayar ita ce nau'in tantancewar da ake amfani da ita don gano tattarawar tantanin halitta da yuwuwa.Maganin shine haɗuwa da acridine orange (koren-fluorescent nucleic acid tabo) da kuma propidium iodide (ja-jajayen nucleic acid tabo).Propidium iodide (PI) wani launi ne na keɓan membrane wanda kawai ke shiga sel tare da membranes masu rikitarwa, yayin da acridine orange yana iya shiga duk sel a cikin yawan jama'a.Lokacin da rinannun biyu ke kasancewa a cikin tsakiya, propidium iodide yana haifar da raguwar acridine orange fluorescence ta hanyar canja wurin makamashi mai haske (FRET).Sakamakon haka, ƙwayoyin sel waɗanda ke da matattun membranes suna lalata kore mai kyalli kuma ana ƙidaya su a matsayin masu rai, yayin da ƙwayoyin sel waɗanda ke da lalatawar membranes kawai suna lalata ja kuma ana ƙidaya su a matsayin matattu lokacin amfani da tsarin Countstar® FL.Abubuwan da ba su da ƙwayar cuta kamar ƙwayoyin jajayen jini, platelets da tarkace ba sa fitowa fili kuma software na Countstar® FL sun yi watsi da su.
Tsarin Farkon Kwayoyin Halitta
Hoto 4 Kulawa da iyawa da ƙididdige ƙwayoyin sel na mesenchymal stem cell (MSCs) don amfani a cikin hanyoyin kwantar da hankulan tantanin halitta.
Ƙayyade iyawar MSC ta AO/PI da Trypan Blue assay
Hoto 2. A. Hoton MSC wanda AO/PI ya yi da kuma Trypan Blue;2. Kwatanta sakamakon AO/PI da Trypan blue kafin da bayan sufuri.
Canje-canjen fihirisar tantanin halitta, Trypan Blue tabon bai fito fili ba, yana da wahala a tantance iyawar bayan sufuri.Yayin da kyalli mai launi biyu ke ba da damar tabo na sel masu rai da matattu, samar da ingantaccen sakamako mai yiwuwa koda a gaban tarkace, platelets, da jajayen ƙwayoyin jini.
