AOPI Dual-fluoresces suav yog hom tshuaj ntsuam xyuas siv los kuaj xyuas cov cell concentration thiab muaj sia nyob.Cov tshuaj yog kev sib xyaw ua ke ntawm acridine txiv kab ntxwv (cov xim ntsuab-fluorescent nucleic acid stain) thiab propidium iodide (liab-fluorescent nucleic acid stain).Propidium iodide (PI) yog ib daim nyias nyias nyias dye uas tsuas yog nkag mus rau hauv cov hlwb nrog kev cuam tshuam daim nyias nyias, thaum acridine txiv kab ntxwv muaj peev xwm nkag mus rau tag nrho cov hlwb hauv cov pejxeem.Thaum ob lub dyes muaj nyob rau hauv lub nucleus, propidium iodide ua rau txo qis hauv acridine txiv kab ntxwv fluorescence los ntawm fluorescence resonance zog hloov (FRET).Raws li qhov tshwm sim, cov cell nucleated nrog cov kab mob tsis zoo stain fluorescent ntsuab thiab suav tias yog nyob, thaum cov cell nucleated nrog cov kab mob cuam tshuam tsuas yog stain fluorescent liab thiab suav tias tuag thaum siv Countstar® FL system.Cov khoom tsis muaj nucleated xws li cov qe ntshav liab, platelets thiab cov khib nyiab tsis muaj fluoresce thiab tsis quav ntsej los ntawm Countstar® FL software.
Txheej txheem ntawm Stem Cell Therapy
Daim duab 4 Kev soj ntsuam ntawm kev muaj peev xwm thiab kev suav ntawm tes ntawm mesenchymal qia hlwb (MSCs) rau kev siv hauv kev kho ntawm tes.
Txiav txim siab MSC kev muaj peev xwm los ntawm AO/PI thiab Trypan Blue assay
Daim duab 2. A. Duab ntawm MSC stained los ntawm AO/PI thiab Trypan Blue;2. Kev sib piv ntawm AO/PI thiab Trypan xiav tshwm sim ua ntej thiab tom qab thauj.
Lub cell refractive index hloov, Trypan Blue staining tsis yog qhov pom tseeb, nws yog qhov nyuaj los txiav txim siab qhov muaj txiaj ntsig tom qab thauj.Thaum ob-xim fluorescence tso cai rau staining ntawm cov nyob thiab tuag nucleated hlwb, ua kom muaj txiaj ntsig zoo txawm tias muaj cov khib nyiab, platelets, thiab cov qe ntshav liab.
