Uhlalutyo lwe-Immuno-phenotyping luvavanyo oluqhelekileyo olwenziwa kumacandelo ophando anxulumene neeseli ukufumanisa izifo ezahlukeneyo (isifo esizimelayo, isifo somzimba, isifo sokuqina kwemithambo-luvo, ukuxilongwa kwethumba, ukopha, izifo ezibangelwa kukwalana, kunye nezinye ezininzi) kunye nesifo sokugula.Ikwasetyenziselwa ukuvavanya umgangatho weseli kuphando lwezifo zeseli ezahlukeneyo.I-cytometry egelezayo kunye ne-fluorescence microscope ziindlela zokuhlalutya rhoqo kumaziko ophando lwezifo zeseli ezisetyenziselwa i-immuno-phenotyping.Kodwa ezi ndlela zokuhlalutya zinokubonelela ngemifanekiso okanye uluhlu lwedatha, kuphela, olungenakuhlangabezana neemfuno ezingqongqo zokuvunywa kwabasemagunyeni.
M Dominici el, Cytotherapy (2006) Vol.8, No. 4, 315-317
Ukuchongwa kwe-Immuno-phenotype ye-AdMSCs
I-immunophenotype ye-AdMSCs igqitywe yi-Countstar FL, i-AdMSCs yafakwa kunye ne-antibody eyahlukeneyo ngokulandelanayo (CD29, CD34, CD45, CD56, CD73, CD105, kunye ne-HLADR).Inkqubo yokufakwa kombala wesiginali yenziwa ngokumisela itshaneli eluhlaza kumfanekiso we-PE fluorescence, kunye nebala eliqaqambileyo.Ulwahlulo lwesalathiso somfanekiso webala eliqaqambileyo lasetyenziswa njengemaski ukwenza isampulu yesiginali ye-PE fluorescence.Iziphumo zeCD105 zaboniswa (Figure 1).
Umzobo 1 Ukuchongwa kwe-Immuno-phenotype ye-AdMSCs.A. Ummandla oqaqambileyo kunye noMfanekiso weFluorescence wee-AdMSC;B. CD Marker Ukufunyanwa kwe-AdMSCs ngu-Countstar FL
Ulawulo lomgangatho wee-MSCs-ukuqinisekisa iziphumo kwiseli nganye nganye
Umzobo 2 A: Iziphumo ze-Countstar FL ziboniswe kwi-FCS Express 5plus, i-gating ipesenteji encomekayo ye-CD105, kunye nokujonga ngokubanzi iiseli zetafile enye.B: Ukulungiswa kwesango lokungena kwicala lasekunene, imifanekiso yetafile yeseli enye ibonisa ezo seli zinembonakalo ephezulu ye-CD105.C: Ukulungiswa kwesango lokungena kwicala lasekhohlo, imifanekiso yetafile yeeseli enye ibonisa ezo seli zinembonakalo ephantsi ye-CD105.
Utshintsho lwePhenotypical ngexesha lezoThutho
Umzobo 3. A: Ucazululo lobungakanani bepesenti elungileyo ye-CD105 kwiisampulu ezahlukeneyo yi-FCS Express 5 kunye nesoftware.B: Imifanekiso ekumgangatho ophezulu ibonelela ngolwazi olongezelelweyo lwe-morphological.C: Iziphumo eziqinisekisiweyo nge-thumbnails yeseli nganye enye, izixhobo ze-software ze-FCS zohlula iiseli ngokwahlukeneyo
