Amaseli we-Mesenchymal stem ayisethi engaphansi yamaseli e-pluripotent stem angahlukaniswa ne-mesoderm.Ngezimpawu zabo zokuvuselela ukuziphindaphinda kanye nezici zokuhlukanisa izinkomba eziningi, banamandla amakhulu ezindlela zokwelapha ezihlukahlukene kwezokwelapha.Amaseli we-Mesenchymal stem ane-phenotype eyingqayizivele ye-immune kanye nekhono lokulawula amasosha omzimba.Ngakho-ke, amaseli e-mesenchymal stem asevele asetshenziswa kabanzi ekushintsheni ama-stem cell, ubunjiniyela bezicubu kanye nokufakelwa kwezitho.Futhi Ngale kwalezi zinhlelo zokusebenza, asetshenziswa njengethuluzi elikahle kubunjiniyela bezicubu njengamaseli embewu ochungechungeni locwaningo oluyisisekelo nolwezempilo.
I-Countstar Rigel ingaqapha ukugxila, ukusebenza, ukuhlaziya i-apoptosis nezici ze-phenotype (kanye nezinguquko zazo) ngesikhathi sokukhiqizwa nokuhlukaniswa kwalawa maseli esiqu.I-Countstar Rigel futhi inenzuzo ekutholeni ulwazi olwengeziwe lwe-morphological, olunikezwa inkambu ekhanyayo engapheli kanye nokuqoshwa kwezithombe okusekelwe ku-fluorescence phakathi nayo yonke inqubo yokuqapha ikhwalithi yeseli.I-Countstar Rigelo inikeza indlela esheshayo, eyinkimbinkimbi nethembekile yokulawula ikhwalithi yamaseli we-stem.
Ukuqapha Ukusebenza Kwe-MSCs Kwezokwelapha Ezivuselelayo
Umfanekiso 1 Ukuqapha ukusebenza nokubala kwamaseli we-mesenchymal stem cell (MSCs) ukuze asetshenziswe ekwelashweni kwamaseli
I-Stem Cell ingenye yezindlela zokwelapha ezithembisayo ekwelashweni kwamaseli okuzivuselela.Kusukela ekuvuneni kwe-MSC kuya ekwelashweni, kubalulekile ukugcina ukusebenza kwe-stem cell ephezulu phakathi nazo zonke izinyathelo zokukhiqizwa kwamaseli e-stem (Umfanekiso 1).Ikhawunta ye-stem cell counter ye-Countstar iqapha ukusebenza kwe-stem cell nokugxila ukuze idlale indima ebalulekile ekulawuleni ikhwalithi.
Ukuqapha Izinguquko ze-MSC Morphological ngemva Kwezokuthutha
Ububanzi nokuhlanganisa kuphinde kwanqunywa ngu-Countstar Rigel.Ububanzi be-AdMSC bushintshwe kakhulu ngemva kokuhamba uma buqhathaniswa nangaphambili kwezokuthutha.Ububanzi bangaphambi kokuthutha babungu-19µm, kodwa bunyuke baba ngu-21µm ngemva kokuthutha.Ukuhlanganiswa kwezinto zangaphambili zokuthutha bekungama-20%, kodwa kukhuphuke kwafinyelela ku-25% ngemuva kokuhamba.Kusukela ezithombeni ezithwetshulwe ngu-Countstar Rigel, i-phenotype ye-AdMSCs yashintshwa kakhulu ngemva kokuhamba.Imiphumela ikhonjiswe kuMfanekiso 3.
Ukuhlonzwa kwama-AdMSC ku-Cell Phenotype
Okwamanje ubuncane bezinqubo zokuhlola ukuhlonza okujwayelekile zokuqinisekisa ikhwalithi yama-MSC agadiwe zisohlwini lwesitatimende se-International Society for Cellular Therapy (ISCT), esichazwe kakade ngo-2006.
Ukutholwa Ngokushesha Kwe-Apoptosis kuma-MSC ane-FITC Conjugated Annexin-V kanye nesingeniso esingu-7-ADD
I-Cell Apoptosis ingatholwa nge-FITC conjugated annexin-V kanye ne-7-ADD.I-PS ivame ukutholakala kuphela epheshaneni elingaphakathi kwe-intracellular le-plasma membrane kumaseli anempilo, kodwa ngesikhathi sokuqala kwe-apoptosis, i-membrane asymmetry iyalahleka futhi i-PS ishintshele epheshaneni langaphandle.
Umfanekiso 6 Ukutholwa kwe-Apoptosis kuma-MSC ngu-Countstar Rigel
A. Ukuhlolwa okubonakalayo kwesithombe se-fluorescence sokutholwa kwe-Apoptosis kuma-MSC
B. Iziqephu ze-Scatter ze-Apoptosis kuma-MSC nge-FCS express
C. Iphesenti lamaseli eseli asekelwe ku-% evamile, % i-apoptotic, kanye % ne-necrotic/amaseli e-apoptotic asekupheleni kakhulu.
