Mesenchymal stem cell ke karoloana ea lisele tsa "pluripotent stem" tse ka arohanngoang le mesoderm.Ka mekhoa ea bona ea ho inchafatsa le ho fapana ka litsela tse ngata, ba na le monyetla o moholo oa liphekolo tse fapaneng tsa bongaka.Lisele tsa stem tsa Mesenchymal li na le phenotype e ikhethang ea 'mele ea ho itšireletsa mafung le matla a taolo ea ho itšireletsa mafung.Ka hona, lisele tsa mesenchymal stem li se li sebelisoa haholo ho fetisetsa lisele tsa stem, boenjiniere ba lisele le ho fetisetsa litho.Ntle le lits'ebetso tsena, li sebelisoa e le sesebelisoa se nepahetseng ho boenjineri ba 'mele e le lisele tsa peo letotong la liteko tsa mantlha le tsa bongaka.
The Countstar Rigel e khona ho shebella mahloriso, bokhoni, tlhahlobo ea apoptosis le litšobotsi tsa phenotype (le liphetoho tsa bona) nakong ea tlhahiso le phapang ea lisele tsena tsa stem.Countstar Rigel e boetse e na le monyetla oa ho fumana tlhaiso-leseling e eketsehileng ea morphological, e fanoeng ke tšimo e khanyang e sa feleng le lirekoto tsa litšoantšo tse thehiloeng ho fluorescence nakong ea ts'ebetso eohle ea ho lekola boleng ba lisele.Countstar Rigelo e fana ka mokhoa o potlakileng, o tsoetseng pele le o tšepahalang bakeng sa taolo ea boleng ba lisele tsa stem.
Ho Lekola Tšebetso ea Li-MSC ho Meriana ea Tsosoloso
Setšoantšo sa 1 Tlhokomelo ea ho sebetsa le palo ea lisele tsa mesenchymal stem cell (MSCs) bakeng sa tšebeliso ea phekolo ea lisele.
Stem Cell ke e 'ngoe ea liphekolo tse ts'episang haholo kalafong ea regenerative cell.Ho tloha kotulong ea MSC ho isa kalafong, ho bohlokoa ho ts'ehetsa ts'ebetso e phahameng ea stem cell nakong ea mehato eohle ea tlhahiso ea lisele tsa stem (setšoantšo sa 1).Countstar's stem cell counter e laola ho sebetsa ha lisele tsa stem le ho tsepamisa mohopolo ho phetha karolo ea bohlokoa taolong ea boleng.
Tlhokomelo ea MSC Morphological Changes ka mor'a Lipalangoang
Bophara le ho kopanya li ne li boetse li khethoa ke Countstar Rigel.Bophara ba li-AdMSC bo fetotsoe haholo kamora lipalangoang ha bo bapisoa le pele ho lipalangoang.Bophara ba pele ho lipalangoang e ne e le 19µm, empa e ile ea nyolohela ho 21µm ka mor'a lipalangoang.Kakaretso ea pele ho lipalangoang e ne e le 20%, empa e nyolohetse ho 25% ka mor'a lipalangoang.Ho tsoa litšoantšong tse nkuoeng ke Countstar Rigel, phenotype ea AdMSCs e fetotsoe haholo kamora lipalangoang.Liphetho li bontšitsoe setšoantšong sa 3.
Khethollo ea li-AdMSC ho Cell Phenotype
Hajoale litekanyetso tse tlase tsa liteko tsa boitsebahatso tsa netefatso ea boleng ba li-MSC tse shebiloeng li thathamisitsoe polelong ea Mokhatlo oa Machaba oa Therapy ea Mehala (ISCT), e hlalositsoeng e seng e ntse e le ka 2006.
Ho lemoha ka potlako ha Apoptosis ho MSC ka FITC Conjugated Annexin-V le 7-ADD Selelekela
Cell Apoptosis e ka bonoa ka FITC conjugated annexin-V le 7-ADD.PS hangata e fumanoa feela letlapeng la intracellular ea plasma liseleng tse phetseng hantle, empa nakong ea apoptosis ea pele, membrane asymmetry ea lahleha mme PS e fetisetsa pampitšaneng e kantle.
Setšoantšo sa 6 Ho fumanoa ha Apoptosis ho MSC ka Countstar Rigel
A. Tlhahlobo ea pono ea setšoantšo sa fluorescence sa Detection of Apoptosis ho MSCs
B. Hasanya merero ea Apoptosis ho MSCs ka FCS express
C. Peresente ea palo ea lisele tse thehiloeng ho % e tloaelehileng, % apoptotic, le % necrotic / morao haholo lisele tsa apoptotic.
