Countstar Mira FL

Izici zomkhiqizo

I-Innovative Optical Multiplication Technology

Ubuchwepheshe bokusondeza obuhlukile buvumela abasebenzisi ukuthi bahlaziye amaseli ngebanga elibanzi lobubanzi

Uma usebenzisa izifanekiso zenkambu ekhanyayo ze-BioApp ku-Countstar Mira, inoveli ethi Zooming Technology inika amandla isisebenzisi ukuhlonza ngokunembile izinto zamaselula ngaphakathi kwebanga le-diamater ukusuka ku-1.0µm ukuya ku-180.0µm.Izithombe ezitholiwe zibonisa imininingwane yeseli elilodwa.Lokhu kunweba ububanzi bezinhlelo zokusebenza ngisho nasezintweni zeselula, ezingakwazanga ukuhlaziya ngokunembile esikhathini esidlule.

Ama-algorithms wokuhlaziya isithombe asekelwe ku-AI

I-Countstar Mira FL isebenzisa izinzuzo ze-Artifical Intelligence ukuthuthukisa ama-algorithms wokuzifundela.Bayakwazi ukuhlonza nokuhlaziya izici eziningi zamaseli.Ukuhlanganiswa kwamapharamitha womumo weseli kuvumela ukuhlaziywa okunembe kakhulu nokuphindaphindekayo kwesimo somjikelezo weseli kanye/noma kulethwa idatha mayelana nokuhlobana phakathi koshintsho ku-morphology yeseli, ukwakheka kwamaqoqo amaseli (amaqoqo, ama-spheroids anosayizi omncane) kanye nezimo ezithintayo.

Ukulebula imiphumela yamaseli e-Mesenchymal Stem Cells (MSC; 5x mangification) anomumo ongajwayelekile) esikweni elikhulayo

• Imibuthano eluhlaza imaka amaseli aphilayo
• Imibuthano ebomvu imaka amaseli afile
• Indilinga emhlophe amaseli ahlanganisiwe

I-RAW264.7 cell line incane futhi igoqeka kalula.I-algorithm ye-Countstar AI ingakwazi ukukhomba amaseli ku-clumps futhi ibale

• Imibuthano eluhlaza imaka amaseli aphilayo
• Imibuthano ebomvu imaka amaseli afile
• Indilinga emhlophe amaseli ahlanganisiwe

Usayizi ongalingani wamaseli e-embryonic we-zebrafish (i-6.6X magnification

• Imibuthano eluhlaza imaka amaseli aphilayo
• Imibuthano ebomvu imaka amaseli afile
• Indilinga emhlophe amaseli ahlanganisiwe

Idizayini ye-Intuitive Graphical User Interface (GUI).

I-GUI ehlelekile ecacile ivumela ukuqaliswa kokuhlolwa okuphumelelayo nokunethezekile

• Umtapo wolwazi obanzi onezinhlobo zamaseli asethwe ngaphambilini kanye ne-BioApps (amaphrothokholi esifanekiso sokuhlola).Ukuchofoza okukodwa nje ku-BioApp, futhi ukuhlolwa kungaqala.
• I-GUI esebenziseka kalula yenza kube lula ukushintsha phakathi kwezinketho ezihlukene zemenyu futhi iqinisekisa ukuzizwisa kokuhlola okunethezekile
• Sula amamojula emenyu ehlelekile asekela umsebenzisi ohlelweni lokuhlola lwansuku zonke

Khetha i-BioApp, faka i-ID Yesampula, bese uqala ukugijima kokuhlola

128 GB womthamo wangaphakathi wedatha yokugcina idatha, eyanele ukugcina cishe.50,000 imiphumela yokuhlaziya ku-Countstar (R) Mira.Ukuze uthole ukufinyelela okusheshayo, idatha efunwayo ingakhethwa ngezinketho ezihlukahlukene zokusesha.

Isici esiwusizo sokonga isikhathi, isibali sokuhlambulula esibuyiselekayo.Izoletha amavolumu anembile esampula yeseli encibilikisiwe neyoqobo, uma ukugxiliswa kokugcina kwamaseli nevolumu eqondiwe sekufakiwe.Lokhu kwenza ukudlula kwamaseli ku-subcultures yabo kube intofontofo.

Izici zohlelo lokusebenza eziningi

Izici zokuhlaziya ze-Countstar Mira zisekela umsebenzisi ekuqondeni izinguquko eziguqukayo ngaphakathi kwesiko leseli futhi kusiza ukukhulisa izimo zabo ezikhulayo.

Isoftware ethuthukisiwe, esekwe ku-AI yokuqaphela isithombe ye-Countstar Mira iyakwazi ukuletha amapharamitha amaningi.Ngaphandle kwemiphumela ejwayelekile yokugxiliswa kwengqamuzana nesimo sokusebenza kahle, ukusatshalaliswa kosayizi weseli, ukwakheka okungenzeka kwamaqoqo amaseli, ukuqina kwe-fluorescence okuhlobene kweseli ngalinye, uhlobo lwejika lokukhula, kanye nesici sawo se-morphology yangaphandle kuyimingcele ebalulekile yokuhlola isimo cell isiko.Amagrafu akhiqizwe ngokuzenzakalelayo amajika okukhula, ukusatshalaliswa kwedayamitha kanye nama-histograms we-fluorescence intensity, ukuhlaziya iseli elilodwa ngaphakathi kwezilinganiso kanye nokunqunywa kwepharamitha yokubumbana kwamaseli kusiza umsebenzisi ukuthi aqonde kangcono izinqubo eziguquguqukayo ngaphakathi kwesiko leseli elihloliwe kusukela ekuqaleni kuya ekupheleni kwenqubo.

I-Histogram

I-Relative Fluorescence Intensity (RFI) yokusabalalisa i-histogram

I-histogram yokusabalalisa ububanzi

Ijika lokukhula

Hlola izithombe kanye nemiphumela

Umdwebo wejika lokukhula

Isicelo somkhiqizo

I-AO/PI dual fluorescence cell density kanye nokuhlolwa kokusebenza

Indlela yokwenza amabala ye-double-fluorescence AO/PI isekelwe kumgomo, wokuthi bobabili odayi, i-Acridine Orange (AO) ne-Propidium Iodide (PI), bayahlangana phakathi kwama-nucleic acid e-chromosome kunucleus yeseli.Nakuba i-AO ikwazi ukungena kulwelwesi olungaguquki lwenucleus nganoma isiphi isikhathi futhi ingcolise i-DNA, i-PI ingadlula kuphela ulwelwesi olusengozini lwenucleus yeseli elifayo (elifile).I-AO eqoqiwe ku-nucleus yeseli ikhipha ukukhanya okuluhlaza okungaphezu kuka-525nm, uma ijatshuliswa ngu-480nm, i-PI ithumela ukukhanya okubomvu nge-amplitude yakho ku-615nm, uma ijabule ku-525nm.Umphumela we-FRET (Foerster Resonance Energy Transfer) uyaqinisekisa, ukuthi isignali ekhishiwe ye-AO ku-525nm imuntshwa phambi kodayi we-PI ukuze kugwenywe ukuphuma kokukhanya okuphindwe kabili nokuchitheka.Le nhlanganisela kadayi ekhethekile ye-AO/PI ivumela ukuhlunga ngokukhethekile i-nucleus equkethe amaseli phambi kwama-acaryote afana nama-erythrocyte.

Idatha ye-Countstar Mira FL ibonise ukuhambisana okuhle kokuhlanjululwa kwe-gradient kwamaseli e-HEK293

Ukuhlaziywa kokusebenza kokudluliselwa kwe-GFP/RFP

Ukusebenza kahle kokudluliselwa kwegciwane kuyinkomba ebalulekile ekuthuthukisweni nasekuthuthukisweni komugqa wamaseli, ekuhlelweni kwe-viral vector, kanye nokuqapha umkhiqizo wemikhiqizo ezinqubweni ze-Biopharma.Sekuyisivivinyo esivame ukusungulwa ukuthola ngokushesha okuthembekile okuqukethwe kwephrotheni okuqondiwe ngaphakathi kweseli.Ezindleleni ezehlukene zokwelapha izakhi zofuzo, iyithuluzi elibalulekile lokulawula ukusebenza kahle kokudluliselwa kofuzo olufunwayo.

I-Countstar Mira ayinikezi kuphela imiphumela enembile nenembile, uma iqhathaniswa ne-cytometry egelezayo, ngaphezu kwalokho i-analyzer inikeza izithombe njengobufakazi bobufakazi.Ngaphandle kwalokhu, yenza kube lula kakhulu futhi kusheshise ukuhlaziya ukuze kube lula ukuthuthukiswa kwenqubo yokuthuthukisa kanye nokukhiqiza.

Uchungechunge lwezithombe, olutholwe yi-Countstar(R) Mira, olubonisa amazinga akhulayo okusebenza kahle kokudluliselwa kwegciwane (kusuka kwesobunxele kuye kwesokudla) kwamaseli aguqulwe ngofuzo (HEK 293 cell line; eveza i-GFP ngokugxila okuhlukene)

Imiphumela yezilinganiso zokuqhathanisa, ezenziwe nge-B/C CytoFLEX, eqinisekisa idatha ye-GFP esebenza kahle yokudluliselwa kwamaseli e-HEK 293 ashintshiwe, ahlaziywa ku-Countstar Mira.

Ukuhlaziywa kokusebenza kwe-Trypan Blue okusungulwe kabanzi

Ukuhlolwa kokubandlululwa kwe-Trypan Blue kusengenye yezindlela ezisetshenziswa kakhulu futhi ezinokwethenjelwa zokunquma inani lamaseli afile (afayo) ngaphakathi kwesiko leseli elimisiwe.Amaseli asebenzayo anesakhiwo solwelwesi seseli esiqinile azoxosha i-Trypan Blue ukuthi ingangeni kulwelwesi.Uma kwenzeka, ulwelwesi lwamaseli luvuza ngenxa yenqubekela phambili yokufa kwengqamuzana layo, i-Trypan Blue ingadlula umgoqo we-membrane, iqoqeke ku-plasma yeseli futhi ingcolise iseli okwesibhakabhaka.Lo mehluko obonakalayo ungasetshenziswa ukuhlukanisa amaseli aphilayo angenasici kumaseli afile ngama-algorithms okubona isithombe we-Countstar Mira FL.

• Izithombe ezintathu, imigqa yamaseli e-Trypan Blue enamabala, etholwe ku-Countstar (R) Mira FL kumodi yensimu ekhanyayo.

• Imiphumela ye-dilution gradient yochungechunge lwe-HEK 293