Ikhaya » Okwe-Bioprocessing

Esingakwenza

  • I-Trypan Blue Cell Counting
  • Ukusebenza nokudluliswa kwe-GFP
  • Ukuhlangana kwama-Antibodies
Trypan Blue Cell Counting
I-Trypan Blue Cell Counting

I-Trypan Blue Cell Counting

Ukuqapha nokuhlaziya isiko lamaseli ngezixazululo zesimanjemanje.Ukuqapha okuthembekile nokusebenzayo kubalulekile ekuthuthukiseni umkhiqizo kanye nekhwalithi yomkhiqizo njengoba ngisho nezinguquko ezincane kumapharamitha we-bioprocess zingathonya ukusebenza kwesiko leseli lakho.Ukubalwa kwamaseli nokusebenza kwayo kuyimingcele ebaluleke kakhulu, i-Countstar® Altair inikeza isixazululo esihlakaniphe ngokwedlulele nesithobelana ngokugcwele nesixazululo se-cGMP salokhu.

Viability and GFP Transfection
Ukusebenza nokudluliswa kwe-GFP

Ngesikhathi se-bioprocess, i-GFP ivame ukusetshenziselwa ukuhlanganisa amaprotheni ahlangene njengenkomba.Nquma i-GFP fluorescent ingabonisa i-protein expression.I-Countstar Rigel inikezela ngesivivinyo esisheshayo nesilula sokuhlola ukudluliselwa kwe-GFP kanye nokusebenza.Amaseli agcotshwe nge-Propidium iodide (PI) kanye ne-Hoechst 33342 ukuze achaze inani lamaseli afile kanye nengqikithi yamaseli.I-Countstar Rigel inikezela ngendlela esheshayo, yobuningi yokuhlola ukusebenza kahle kokuvezwa kwe-GFP nokusebenza ngesikhathi esifanayo.

Antibodies Affinity
Ukuhlangana kwama-Antibodies
Amasosha omzimba ahlobene ngokuvamile akalwa ngu-Elisa noma i-Biacore, lezi zindlela zizwela kakhulu, kodwa zibona i-antibody ngephrotheni ehlanzekile, kodwa hhayi iphrotheni yemvelo yemvelo.Sebenzisa indlela ye-cell immunofluorescence, umsebenzisi angakwazi ukubona ukuhlobana kwe-antibody neprotheyini ye-conformation yemvelo.Njengamanje, ukulinganisa kokuhambisana kwe-antibody kuhlaziywa nge-flow cytometry.I-Countstar Rigel futhi inganikeza indlela esheshayo nelula yokuhlola ukuhlobana kwe-antibody.
I-Countstar Rigel ingathwebula ngokuzenzakalelayo isithombe kanye nenani lokuqina kwe-fluorescence okungabonisa ukuhambisana kwe-antibody.

Imikhiqizo Enconyiwe

Izinsiza Ezihlobene

Ubumfihlo bakho bubalulekile kithi.

Sisebenzisa amakhukhi ukuthuthukisa ulwazi lwakho lapho uvakashela amawebhusayithi ethu: amakhukhi okusebenza asibonisa ukuthi uyisebenzisa kanjani le webhusayithi, amakhukhi asebenzayo akhumbula okuncamelayo futhi amakhukhi aqondisayo asisiza ukuthi sabelane ngokuqukethwe okuhlobene nawe.

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