Falanqaynta Immuno-phenotyping waa tijaabo caadi ah oo lagu sameeyo qaybaha cilmi-baarista ee unugyada si loo ogaado cudurro kala duwan (cudurka difaaca jirka, cudurrada difaaca jirka, ogaanshaha burada, hemostasis, cudurrada xasaasiyadda, iyo kuwo kale oo badan) iyo cudurrada cudurrada.Waxa kale oo loo isticmaalaa in lagu tijaabiyo tayada unugyada cilmi-baarista cudurrada unugyada kala duwan.Cytometry socodka iyo mikroskoobyada fluorescence waa hababka falanqaynta joogtada ah ee cudurrada unugyada machadyada cilmi-baarista ee loo isticmaalo difaaca-fenotyping.Laakiin hababkan falanqayntu waxay bixin karaan sawirro ama taxane xog, kaliya, kuwaas oo aan buuxinayn shuruudaha oggolaanshaha adag ee masuuliyiinta sharciyeynta.
Mi Dominici el, Cytotherapy (2006) Vol.8, No. 4, 315-317
Aqoonsiga Immuno-phenotype ee AdMSCs
Nooca difaaca jirka ee AdMSCs waxaa go'aamiyay Countstar FL, AdMSCs waxaa lagu daray antibody kala duwan siday u kala horreeyaan (CD29, CD34, CD45, CD56, CD73, CD105, iyo HLADR).Nidaamka codsiga midabka calaamadda ayaa la sameeyay iyadoo la dejinayo kanaalka Cagaaran si uu u sawiro PE fluorescence, oo lagu daray goob dhalaalaya.Qaybta tixraaca sawirka bannaanka ee dhalaalaya ayaa loo adeegsaday sidii maaskaro si loo muunado calaamadda fluorescence ee PE.Natiijooyinka CD105 ayaa la muujiyay (Jaantuska 1).
Jaantuska 1 Aqoonsiga Immuno-phenotype ee AdMSCs.A. Goobta Iftiinka leh iyo Sawirka Fluorescence ee AdMSCs;B. Soo saarista CD-ga ee AdMSCs ee Countstar FL
Xakamaynta tayada MSC-yada - xaqiijinta natiijooyinka unug kasta
Jaantuska 2 A: Natiijooyinka Countstar FL waxa lagu soo bandhigay FCS express 5plus, iyaga oo helaya boqolkiiba togan ee CD105, iyo dulmarka miiska hal unug.B: Albaabka la isku hagaajiyay ee dhinaca midigta ah, sawirada miiska hal unug ayaa muujinaya unugyadaas muujinta sare ee CD105.C: Albaabka la isku hagaajiyay ee dhinaca bidixda ah, sawirada miiska unugyadu waxay muujinayaan unugyadaas muujinta hoose ee CD105.
Isbeddellada phenotypical inta lagu jiro gaadiidka
Jaantus 3. A: Falanqaynta tirada ee boqolkiiba togan ee CD105 ee muunado kala duwan ee FCS express 5 iyo software.B: Sawirro tayo sare leh ayaa bixiya macluumaad maskaxeed oo dheeraad ah.C: Natiijooyinka lagu xaqiijiyay thumbnails unug kasta, aaladaha software FCS waxay u qaybiyeen unugyo kala duwan
