Immuno-phenotyping ongororo kuyedza kunoitwa mumasero ane chekuita nekutsvagisa minda yekuongorora zvirwere zvakasiyana (autoimmune chirwere, immunodeficiency chirwere, tumor kuongororwa, hemostasis, allergic zvirwere, nezvimwe zvakawanda) uye chirwere chechirwere.Inoshandiswawo kuyedza mhando yesero mune dzakasiyana siyana dzechirwere chekuongorora.Flow cytometry uye fluorescence microscope ndiyo nzira dzekuongorora dzechinyakare mumaseru zvirwere masangano ekutsvagira anoshandiswa immuno-phenotyping.Asi idzi nzira dzekuongorora dzinogona kupa mifananidzo kana data nhevedzano, chete, iyo inogona kusasangana neyakaomesesa yemvumo zvinodiwa nevakuru vanotonga.
M Dominici el, Cytotherapy (2006) Vol.8, Nhamba 4, 315-317
Kuzivikanwa kweImmuno-phenotype yeAdMSCs
Iyo immunophenotype yeAdMSCs yakatemerwa neCountstar FL, AdMSCs akaiswa neanorwisa antibody akasiyana zvakateerana (CD29, CD34, CD45, CD56, CD73, CD105, uye HLADR).Iyo sign-color application maitiro akagadzirwa nekuisa Green chiteshi kumufananidzo PE fluorescence, pamwe nemunda wakajeka.Bright field reference segmentation yakashandiswa semask kuenzanisa chiratidzo chePE fluorescence.Migumisiro yeCD105 yakaratidzwa (Mufananidzo 1).
Mufananidzo 1 Kuzivikanwa kweImmuno-phenotype yeAdMSCs.A. Munda Wakajeka uye Fluorescence Mufananidzo weAdMSCs;B. CD Marker Kuonekwa kweAdMSCs neCountstar FL
Kudzora kwemhando yeMSCs - kusimbisa mhedzisiro yesero rega rega
Mufananidzo 2 A: Iyo Countstar FL mhedzisiro yakaratidzwa muFCS express 5plus, ichiisa iyo yakanaka muzana yeCD105, uye yekutarisa tafura masero mamwechete.B: Yakagadziridzwa gedhi kudivi rekurudyi, mifananidzo yesero rimwe chete tafura inoratidza iwo maseru ane yakakwirira kutaura kweCD105.C: Yakagadziridzwa gedhi kuruboshwe, mifananidzo yemaseru maseru tafura inoratidza iwo maseru ane yakaderera kutaura kweCD105.
Phenotypical Changes panguva Yekufambisa
Mufananidzo 3. A: Kuongororwa kwehuwandu hwechikamu chakanaka cheCD105 mumasampuli akasiyana neFCS express 5 plus software.B: Mifananidzo yemhando yepamusoro inopa mamwe morphological ruzivo.C: Mhedzisiro yakagadziriswa nezvigunwe zvesero rimwe nerimwe, FCS software maturusi akakamura maseru kuita akasiyana
