Immuno-phenotyping su'esu'ega ose fa'ata'ita'iga masani e fa'atino i vaega su'esu'e e feso'ota'i sela e su'esu'e ai fa'ama'i eseese (fa'ama'i autoimmune, fa'ama'i immunodeficiency, su'esu'ega tumo, hemostasis, fa'ama'i fa'ama'i, ma le tele o isi mea) ma fa'ama'i fa'ama'i.E fa'aaogaina fo'i e su'e ai le tulaga lelei ole sela i su'esu'ega fa'ama'i eseese.Flow cytometry ma fluorescence microscope o auala masani ia au'ili'ili i totonu o fale su'esu'e fa'ama'i sela fa'aoga mo le immuno-phenotyping.Ae o nei metotia auʻiliʻili e mafai ona tuʻuina atu ata poʻo faʻasologa o faʻamaumauga, naʻo, atonu e le fetaui ma manaʻoga faʻamaonia a le pulega faʻatonutonu.
M Dominici el, Cytotherapy (2006) Vol.8, Nu. 4, 315-317
Fa'ailoaina ole Immuno-phenotype o AdMSCs
O le immunophenotype o AdMSCs na fuafuaina e Countstar FL, AdMSCs na faʻapipiʻiina i antibody eseese (CD29, CD34, CD45, CD56, CD73, CD105, ma HLADR).Na faia se faiga fa'ailoga lanu e ala i le fa'atulagaina o le alalaupapa Green i le ata PE fluorescence, fa'atasi ai ma se fanua susulu.Na fa'aogaina le vaeluaga o fa'amatalaga fa'amatalaga o le fanua e fai ma ufimata e fa'ata'ita'i ai le fa'ailoga fa'alili PE.O faʻaiʻuga o le CD105 na faʻaalia (Ata 1).
Ata 1 Fa'ailoaina ole Immuno-phenotype o AdMSCs.A. Malaga Malamalama ma Ata Fluorescence o AdMSCs;B. Fa'ailoga CD Su'esu'ega o AdMSCs e Countstar FL
Pulea lelei o MSC - fa'amaonia iuga mo sela ta'itasi
Ata 2 A: O fa'ai'uga o le Countstar FL na fa'aalia ile FCS express 5plus, fa'atasi ai le pasene lelei o le CD105, ma le va'aiga lautele o sela ta'itasi.B: Fetuuna'i gating i le itu taumatau, o ata o le laulau sela tasi o loʻo faʻaalia ai na sela e maualuga le faʻaaliga o le CD105.C: Fetuuna'i gating i le itu agavale, o ata o le laulau sela tasi o loʻo faʻaalia ai na sela e maualalo le faʻaaliga o le CD105.
Suiga Phenotypical i le taimi o Felauaiga
Ata 3. A: Su'esu'ega fa'atatau o le pasene lelei o le CD105 i fa'ata'ita'iga 'ese'ese e le FCS express 5 fa'aopoopo polokalame.B: O ata sili ona lelei e maua ai fa'amatalaga fa'aopoopo fa'apitoa.C: Fa'amaonia fa'ai'uga e la'ititi o sela ta'itasi, o meafaigaluega faakomepiuta FCS vaevae sela i ni 'ese'ese
