Isampulu equlathe iiseli ezixhonyiweyo ixutywa nedayi ye-Trypan eluhlaza okwesibhakabhaka, emva koko izotywe kwi-Countstar Chamber Slide ehlalutywe yi-Countstar Automated Cell Counter.Ngokusekwe kumgaqo wokubala iiseli ze-trypan eluhlaza okwesibhakabhaka, izixhobo ze-Countstar zidibanisa itekhnoloji ye-imaging ye-optical, itekhnoloji ekrelekrele yokuqaphela umfanekiso kunye ne-algorithms yesoftware enamandla ukuba inganikezeli nje ukugxila kweeseli kunye nokusebenza, kodwa ikwabonelela ngolwazi lokuxinana kweeseli, ukusebenza, izinga lokudityaniswa, ukujikeleza. , kunye nonikezelo lwedayamitha ngokuqhutywa kanye kuphela.
Uhlalutyo lweSeli oludityanisiweyo
Umzobo 3 Ukubalwa kweeseli ezidityanisiweyo.
A. Umfanekiso wesampulu yeSeli;
B. Umfanekiso weSampuli yeSeli enophawu lokuchongwa yi-software ye-Countstar BioTech.(Isangqa esiLuhlaza: Iseli ephilayo, isangqa esimthubi: Iseli efileyo, isangqa esiBomvu: iSeli edibeneyo).
C. I-Aggregated Histogram
Ezinye iiseli zeprayimari okanye iiseli ze-subculture zidla ngokudibana xa imeko yenkcubeko ihlwempuzekileyo okanye ukugaya kakhulu, oko kubangela ubunzima obukhulu ekubaleni iiseli.NgoMsebenzi woQoqosho lokuQinisekisa, i-Countstar inokuqonda ukubalwa kovuselelo lwee-aggregations ukuqinisekisa ukubalwa kweeseli ezichanekileyo kunye nokufumana ireyithi yokudibanisa kunye ne-histogram yokudibanisa, ngaloo ndlela ibonelela ngesiseko sabenza umfuniselo ukugweba imeko yeeseli.
Ukubekwa esweni kokuKhula kweeSeli
Umzobo we-4 weSeli yokuKhula iCwave.
Igophe lokukhula kweseli yindlela eqhelekileyo yokulinganisa ukukhula ngokupheleleyo kwenani leseli, isalathisi esibalulekileyo sokumisela ukuxinana kweeseli kunye nenye yeeparamitha ezisisiseko zenkcubeko yeempawu zebhayoloji ezisisiseko zeeseli.Ukuze uchaze ngokuchanekileyo utshintsho oluguquguqukayo kwinani leeseli kuyo yonke inkqubo, ijika eliqhelekileyo lokukhula lingahlulwa libe ngamacandelo ama-4: ixesha lokufukamela kunye nokukhula okucothayo;Inqanaba lokukhula okubonakalayo kunye nethambeka elikhulu, isigaba sethafa kunye nexesha lokuhla.Ijika lokukhula kweeseli lingafumaneka ngokucwangcisa inani leeseli eziphilayo (10'000 / mL) ngokubhekiselele kwixesha lenkcubeko (h okanye d).
Ukulinganisa Ukugxila kwiSeli kunye nokuSebenza
Umzobo we-1 Imifanekiso ibanjwe yi-Countstar BioTech njengeeseli (i-Vero, i-3T3, i-549, i-B16, i-CHO, i-Hela, i-SF9, kunye ne-MDCK) ekumisweni yayigxininiswe yi-Trypan Blue ngokulandelanayo.
I-Countstar isetyenziswa kwiiseli ezinobubanzi obuphakathi kwe-5-180um, njengeseli ye-mammalian, iseli yezinambuzane, kunye nezinye iiplankton.
Ubungakanani beSeli yokulinganisa
Umzobo we-2 Ubungakanani beSeli Ukulinganisa ubungakanani beeseli ze-CHO ngaphambi nangemva kokudluliselwa kweplasmid.
A. Imifanekiso yeeseli ze-CHO zinqunyanyiswa yi-trypan eluhlaza okwesibhakabhaka ngaphambi nangemva kokudluliselwa kweplasmid.
B. Ukuthelekiswa kwe-CHO ye-cell size histogram ngaphambi nangemva kokudluliselwa kwe-plasmid.
Ukutshintsha kobungakanani beeseli luphawu oluphambili kwaye luqhele ukulinganiswa kuphando lweeseli.Ngokuqhelekileyo kuya kulinganiswa kolu vavanyo: ukudluliselwa kweeseli, uvavanyo lweziyobisi kunye novavanyo lokusebenzisa iseli.I-Countstar ibonelela ngedatha ye-morphology yezibalo, ezifana nobukhulu beeseli, ngaphakathi kwe-20s.
Ikhawuntala yeeseli ezizihambelayo ze-Countstar inokunika idatha yemopholoji yeeseli, kubandakanywa ukujikeleza kunye neehistograms zedayamitha.
