Muenzaniso une maseru ekusimudzwa uri kusanganiswa neTrypan dhayi yebhuruu, yobva yadhonzwa muCountstar Chamber Slide yakaongororwa neCountstar Automated Cell Counter.Kubva pane yekare trypan bhuruu cell counting musimboti, Countstar's zviridzwa zvinosanganisa advanced optical imaging tekinoroji, yakangwara yekuona mufananidzo tekinoroji uye ine simba software algorithms kwete kungopa iyo sero kusungwa uye kushanda, asi zvakare inopa ruzivo rwekusemburwa kwesero, kugona, chiyero chekuunganidza, kutenderera. , uye kugovera dhayamita nekumhanya kumwe chete.
Aggregated Cell Analysis
Mufananidzo 3 Kuverenga kwemasero akaunganidzwa.
A. Mufananidzo weSero muenzaniso;
B. Mufananidzo weSero Sample ine chitupa chiratidzo neCountstar BioTech software.(Green Circle: Live cell, Yellow Circle: Dead Sero, Red Circle: Aggregated Cell).
C. Aggregated Histogram
Mamwe maseru ekutanga kana maseru etsika anowanzo kuunganidza kana tsika isina kunaka yamira kana kugaya zvakanyanya, zvichikonzera kuoma kukuru mukuverenga masero.NeAggregation Calibration Function, Countstar inogona kuona kurudziro kuverenga kweaggregations kuti ive nechokwadi chekuverenga maseru uye kuwana chiyero chekubatanidza uye aggregation histogram, zvichipa hwaro hwevanoedza kutonga mamiriro emasero.
Kuongorora Kukura Kwesero
Mufananidzo 4 Sero Rikure Curve.
Kukura kwesero curve inzira yakajairika yekuyera kukura kwakazara kwenhamba yesero, chiratidzo chakakosha chekuona kusungwa kwesero uye imwe yeakakosha maparamita etsika yezvinhu zvebhayoloji zvemasero.Kuti unyatso tsanangura shanduko ine simba muhuwandu hwemasero mukati mekuita kwese, iyo yakajairika yekukura curve inogona kukamurwa kuita zvikamu zvina: incubation nguva nekukura kunonoka;exponential growth phase ine mutsetse wakakura, plateau phase uye kudzikira nguva.Iyo sero yekukura curve inogona kuwanikwa nekuronga huwandu hwemasero mapenyu (10'000/mL) maererano nenguva yetsika (h kana d).
Kuyera Sero Concentration uye Viability
Mufananidzo 1 Mifananidzo yakatorwa neCountstar BioTech semasero (Vero, 3T3, 549, B16, CHO, Hela, SF9, uye MDCK) mukumiswa yakasvibiswa neTrypan Blue zvakateerana.
Countstar inoshandiswa kumaseru ane dhayamita ari pakati pe5-180um, senge mammalian sero, sero rezvipembenene, uye mamwe mapurani.
Sero Kuyera Kuyera
Mufananidzo 2 Cell Size Kuyera kweCHO masero pamberi uye mushure mekutapurirana kweplasmid.
A. Mifananidzo yeCHO masero akamiswa akasvibiswa netrypan yebhuruu pamberi uye mushure mekutapurirana kweplasmid.
B. Kufananidza kweCHO cell size histogram isati yasvika uye mushure mekutapurirwa kweplasmid.
Shanduko yehukuru hwesero chinhu chakakosha uye inowanzoyerwa mukutsvaga kwesero.Kazhinji ichayerwa mune izvi zviedzo: kuchinjisa masero, bvunzo yezvinodhaka uye maseru activation assays.Countstar inopa nhamba ye morphology data, senge hukuru hwemasero, mukati memakore makumi maviri.
Countstar automated cell counter inogona kupa iyo morphological data yemasero, kusanganisira kutenderera uye dhayamita histograms.
