Hoʻohui ʻia kahi laʻana i loko o nā cell i hoʻokuʻu ʻia me Trypan blue dye, a laila huki ʻia i loko o ka Countstar Chamber Slide i kālai ʻia e Countstar Automated Cell Counter.Ma muli o ka trypan blue cell counting principle, Countstar's instruments integrate advanced optical imaging technology, intelligent image recognition technology and powerful software algorithms to provide the cell concentration and viability, but also provide the information of cell concentration, viability, aggregation rate, roundness. , a me ka mahele anawaena me ka holo hookahi wale no.
ʻIkepili Pūnaewele Hui
Kiʻi 3 Ka helu ʻana o nā pūnaewele i hōʻuluʻulu ʻia.
A. Kiʻi o Cell sample;
B. Kiʻi o Cell Sample me ka hōʻailona ʻike e ka polokalamu Countstar BioTech.(Green Circle: Live cell, Yellow Circle: Dead Cell, Red Circle: Aggregated Cell).
C. Hōʻuluʻulu Histogram
Hiki ke hōʻuluʻulu ʻia kekahi mau cell mua a i ʻole subculture cell i ka wā o ka moʻomeheu maikaʻi ʻole a i ʻole ka ʻai nui ʻana, no laila e pilikia nui ai ka helu ʻana o nā cell.Me ka Aggregation Calibration Function, hiki i ka Countstar ke hoʻomaopopo i ka helu hoʻoulu ʻana o nā hōʻuluʻulu e hōʻoia i ka helu kelepona pololei a loaʻa ka helu aggregation a me ka histogram aggregation, no laila e hāʻawi i kumu no nā mea hoʻokolohua e hoʻoponopono i ke kūlana o nā cell.
Ka kiaʻi ʻana i ka ulu ʻana o ke kelepona
Kiʻi 4 Cell Grow Curve.
He ʻano maʻamau ka pihi ulu ʻana o ka cell no ke ana ʻana i ka ulu piha ʻana o ka helu kelepona, kahi hōʻailona koʻikoʻi e hoʻoholo ai i ka neʻe ʻana o ka cell a me kekahi o nā ʻāpana kumu no ka moʻomeheu o nā waiwai olaola kumu o nā cell.I mea e wehewehe pono ai i ka hoʻololi ikaika i ka helu o nā cell i loko o ke kaʻina holoʻokoʻa, hiki ke hoʻokaʻawale ʻia ka pihi ulu maʻamau i 4 mau ʻāpana: ka wā incubation me ka ulu lohi;ka wā ulu exponential me ka piʻi nui, ka pae pāpū a me ka wā emi.Hiki ke loaʻa ka pihi ulu o ke kelepona ma ka hoʻolālā ʻana i ka helu o nā cell ola (10'000/mL) e kūʻē i ka manawa moʻomeheu (h a i ʻole d).
Ke ana i ka helu kelepona a me ka hiki
Kiʻi 1 Ua kiʻi ʻia nā kiʻi e Countstar BioTech ma ke ʻano he mau cell (Vero, 3T3, 549, B16, CHO, Hela, SF9, a me MDCK) i ka hoʻokuʻu ʻia ʻana e Trypan Blue.
Hoʻohana ʻia ka Countstar i nā cell me ke anawaena ma waena o 5-180um, e like me ka cell mammalian, cell insect, a me kekahi mau plankton.
Ana Nui Cell
Kiʻi 2 ʻO ke ana ʻana o ka nui o nā cell CHO ma mua a ma hope o ka hoʻololi plasmid.
A. Nā kiʻi o ka hoʻokuʻu ʻia ʻana o nā keʻena CHO i hoʻopaʻa ʻia e ka trypan blue ma mua a ma hope o ka hoʻololi ʻana i ka plasmid.
B. Ka hoʻohālikelike ʻana o ka histogram ka nui o ka cell CHO ma mua a ma hope o ka hoʻololi ʻana i ka plasmid.
ʻO ka hoʻololi ʻana o ka nui o ke kelepona kahi hiʻohiʻona nui a ua ana maʻamau i ka noiʻi kelepona.ʻO ka maʻamau e ana ʻia i loko o kēia mau hoʻokolohua: ka hoʻololi ʻana o ke kelepona, ka hoʻāʻo lāʻau a me ka hoʻāʻo ʻana o ke kelepona.Hāʻawi ʻo Countstar i ka ʻikepili morphology helu, e like me ka nui o nā cell, i loko o 20s.
Hiki iā Countstar ke hāʻawi i ka ʻikepili morphological o nā cell, me ka circularity a me ke anawaena histograms.
