Loaʻa i ke koko a me nā pūnaewele mua i hoʻokaʻawale hou ʻia a i ʻole nā pūnaewele moʻomeheu i loko o nā mea haumia, nā ʻano cell a i ʻole nā mea hoʻopilikia e like me nā ʻōpala cell e hiki ʻole ai ke kālailai i nā keena hoihoi.Hiki i ka Countstar FL me ka nānā ʻana i ke ʻano fluorescence ʻelua ke hoʻokaʻawale i nā ʻāpana cell, nā ʻōpala a me nā ʻāpana artifact a me nā hanana undersized e like me nā platelets, e hāʻawi ana i kahi hopena pololei loa.
AO/PI Dual Fluorescence Viability Helu
ʻO ka ʻalani Acridine (AO) a me ka Propidium iodide (PI) nā mea hoʻopaʻa paʻa nukleika nukleika.Hoʻokuʻu ʻia ka ʻikepili i nā ʻāpana cell, nā ʻōpala a me nā ʻāpana artifact a me nā hanana undersized e like me ke koko ʻulaʻula, e hāʻawi ana i kahi hopena pololei loa.I ka hopena, hiki ke hoʻohana ʻia ka ʻōnaehana Countstar no kēlā me kēia pae o ke kaʻina hana kelepona.
Nā WBC i ke koko holoʻokoʻa
Kiʻi 2 Kiʻi laʻana koko holoʻokoʻa i hopu ʻia e Countstar Rigel
ʻO ka nānā ʻana i nā WBC i loko o ke koko holoʻokoʻa he hoʻomaʻamaʻa maʻamau i loko o ke keʻena lapaʻau a i ʻole ka waihona koko.ʻO ka noʻonoʻo ʻana a me ka hiki ʻana o nā WBC ka mea nui e like me ka mālama ʻana i ke koko.
Hiki i ka Countstar Rigel me ke ʻano AO/PI ke hoʻokaʻawale pololei i ke kūlana ola a make o nā cell.Hiki i ka Rigel ke hana pololei i ka helu WBC me ka haʻalele ʻana i ka hoʻopili ʻana o nā ʻāpana koko ʻulaʻula.
Helu a me ka Viability o PBMC
Kiʻi 3 Nā kiʻi Bright Field a me Fluorescence o ka PBMC i hopu ʻia e Countstar Rigel
ʻO ka helu AOPI Dual-fluoresces ka ʻano assay i hoʻohana ʻia no ka ʻike ʻana i ka neʻe ʻana o ke kelepona a me ka hiki.ʻO ka hopena, ʻo nā pūnaewele nucleated me nā membrane paʻa i hoʻopaʻa i ka ʻōmaʻomaʻo ʻōmaʻomaʻo a helu ʻia he ola, ʻoiai nā cell nucleated me nā membrane i hoʻopaʻapaʻa wale ʻia ka ʻulaʻula fluorescent a helu ʻia he make ke hoʻohana ʻia ka ʻōnaehana Countstar Rigel.ʻAʻole ʻike ʻia nā mea non-nucleated e like me ke koko ʻulaʻula, platelets a me nā ʻōpala a nānā ʻole ʻia e ka polokalamu Countstar Rigel.
