Ukusebenzisa i-Cytometer yoMfanekiso uPhuculo loPhuhliso lweSeli kwi-Biologics kunye ne-rAAV Production

I-Biologics kunye ne-AAV-based gene therapies ifumana isabelo esingakumbi semarike kunyango lwezifo.Nangona kunjalo, ukuphuhlisa umgca weseli owomeleleyo kunye nosebenzayo we-mammalian kwimveliso yabo kulucelomngeni kwaye ngokuqhelekileyo kufuna ukulinganiswa okubanzi kweeseli.Ngokwembali, i-flow cytometer isetyenziswa kolu vavanyo lusekwe kwiseli.Nangona kunjalo, i-cytometer yokuhamba iyabiza kwaye ibandakanya uqeqesho olubanzi ekusebenzeni kunye nokugcinwa.Kungekudala, ngokunyuka kwezakhono zekhompyutheni kunye neekhamera eziphezulu zekhamera, i-cytometry-based based based cytometry iye yahlaziywa ukuze ibonelele ngenye indlela echanekileyo neyindleko yokuphuhliswa kwenkqubo yeseli.Kulo msebenzi, sichaze ukuhamba komsebenzi wokuphuhlisa umgca weseli obandakanya i-cytometer esekelwe kumfanekiso, ebizwa ngokuba yi-Countstar Rigel, yokuvavanya ukuhanjiswa kwe-transfection kunye novavanyo oluzinzileyo lwe-pool usebenzisa i-CHO kunye neeseli ze-HEK293 ezibonisa i-antibody kunye ne-rAAV vector, ngokulandelanayo.Kwimizekelo emibini yophononongo, sibonise:

1. I-Countstar Rigel inike ukuchaneka okufanayo kokufumanisa ukuhamba kwe-cytometry.
2. I-Countstar Rigel-based pool evaluation inokunceda ukugqiba iqela elinqwenelekayo le-single-cell cloning (SCC).
3. I-Countstar Rigel ifake iqonga lophuhliso lomgca weseli iphumelele i-2.5 g/L mAb titer.

Siye saxoxa nangokunokwenzeka kokusebenzisa i-Countstar njengolunye umaleko we-rAAV-based optimization target.

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