Intshayelelo
Ukulinganisa ukufakwa kwe-DNA-binding dyes ibe yindlela esekwe kakuhle yokumisela umxholo we-DNA yeselula kuhlalutyo lomjikelo weeseli.IPropidium iodide (PI) yidayi engcolisa inyukliya esetyenziswa rhoqo xa kumetwa umjikelo weseli.Kulwahlulo lweeseli, iiseli eziqulethe izixa ezandisiweyo ze-DNA zibonisa ngokulinganayo ukwanda kwe-fluorescence.Umahluko kwi-fluorescence intensity isetyenziselwa ukumisela umxholo we-DNA kwinqanaba ngalinye lomjikelo weseli.Inkqubo ye-Countstar Rigel (Fig.1) iyisilumko esihlakaniphile, esicacileyo, esinemisebenzi emininzi esinokuthi sifumane idatha echanekileyo kwi-cell cycle analysis kwaye inokubona i-cytotoxicity nge-cell viability assay.Ekulula ukuyisebenzisa, inkqubo ezenzekelayo ikhokela ukuba ugqibezele uvavanyo lweselula ukusuka kumfanekiso kunye nokufumana idatha.
