Blood and freshly isolated primary cells or cultured cells may contain impurities, several cell types or interfering particles such as cell debris which will make it impossible to analyze the cells of interest. Countstar FL with dual fluorescence method analysis can exclude cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result.
AO/PI Dual Fluorescence Viability Counting
Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as red blood cell, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.
WBCs in Whole Blood
Figure 2 Whole blood sample image captured by Countstar Rigel
Analyzing the WBCs in whole blood is a routine assay in a clinical lab or blood bank. The concentration and viability of the WBCs are the vital index as quality control of blood storage.
The Countstar Rigel with AO/PI method can accurately distinguish the live and dead state of cells. The Rigel can also can do the WBC count accurately while excluding the interference of red blood cells.
Counting and Viability of PBMC
Figure 3 Bright Field and Fluorescence images of the PBMC Captured by Countstar Rigel
AOPI Dual-fluoresces counting is the assay type used for detecting cell concentration and viability. As a result, nucleated cells with intact membranes stain fluorescent green and are counted as live, whereas nucleated cells with compromised membranes only stain fluorescent red and are counted as dead when using the Countstar Rigel system. Non-nucleated material such as red blood cells, platelets and debris do not fluoresce and are ignored by the Countstar Rigel software.
