Countstar Rigel S3 Fluorescence Cell Analyzer

Countstar® Rigel S3
Fluorescence Cell Analyzer

  • CAR-T Cell cell mediated Cytotoxicity Studies
  • Phenotypic Charaterization of CAR-T cells
  • Viability and Concentration assay for :
    1. Quality control of original blood samples
    2. PBMC isolation efficiency tests
    3. Cultured status of incubated T-lymphocytes
    4. Process Monitoring of modified CAR-T cells
    5. Final Product released control.

Product Overview

The Countstar S3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks,which leaves you time to focus on the research!

Excitation light: 375nm, 480nm, 525nm 
Emission filter:460nm, 535nm, 600LP

Key Benefits

  • Integrated design computer / touch screen.
  • Automatic Detection 5 Samples in one single test sequence.
  • Open platform with maximum 4 fluorescence Channel
  • User Friendly Cell Analysis Protocols
  • Unrivaled "Fixed focus" patent, no manual focusing.
  • High Stability and Actuary
  • Powerful and user-friendly Data Management
  • GMP and 21 CFR part 11 ready
  • FCS Express Software
  • Trypan blue protocol for cell counting.
  • Fluorescence Cell Analyzer.
Product feature
Up to 4 fluorescent channel and one bright field analysis at the same time
Up to 4 fluorescent channel and one bright field analysis in the same time

13 different fluorescent analysis combinations

13 different fluorescent analysis combinations

User Friendly Cell Cycle Analysis Protocols

User Friendly Cell Analysis Protocols

Trypan blue ProtocolsTrypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.
AO/PI Viability ProtocolAO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.
Affinity of AntibodyAffinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.
Surface Marker AssaySurface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)
Cell CycleCell Analysis: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycles. This protocol determines cellular DNA content in cell cycle analysis.
Cell ApoptosisCell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.
GFP transfectionGFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.
Celling KillingCelling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

GMP and 21 CFR part 11 ready

Make a detailed record of any changes, edits, deletions, adjustments, etc. Electronic records cannot be deleted, including administrators. Electronic records include date, time, user name, machine serial number, and user's actions

GMP and 21 CFR part 11 ready

FCS Express Software

The optional De NovoTM FCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar FL together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

FCS Express Software

 Technical Specifications
Model: Countstar Rigel S3
Diameter range: 3μm ~ 180μm
Concentration range: 1×104 ~ 3×107/mL
Objective magnification: 5x
Imaging element: 1.4 megapixel ,CCD camera
Excitation Light 375nm, 480nm, 525nm
Emission Filter 460nm, 535nm, 600LP
USB 1×USB 3.0 1×USB 2.0
Storage: 500GB
Power supply: 110–230 V/AC, 50/60Hz
Screen: 10.4 inch touchscreen
Weight: 13kg (28lb)
Size (W X D X H): Machine: 254×303×453mm Package size: 430×370×610mm
Operating temperature: 10℃ ~ 40℃
Working humidity: 20% ~ 80%
Dual-Fluorescence Viability:
Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar system can be used for every step of the cell manufacturing process.

Dual-Fluorescence Viability
Dual-Fluorescence Viability

T/NKcell mediatedcytotoxicity
By labeling the target tumor cells with CFSE or transfect with GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

T/NK cell mediated cytotoxicity
Live tumor cell | Dead tumor cell | Live T cell | Dead T cell

Cell Apoptosis
The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® FL offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell Apoptosis

Cell Cycle
Countstar Rigel S6 enables users to get the result of a cell cycle quickly and accurately. Counstar Rigel S6 can analyze proportion of cell in different phase of the cell cycle.

Cell Cycle
Surface Maker Analysis
A lymphocyte subsets analysis is a typical experiment performed in cell related research fields and various diseases diagnosis. Countstar® Rigel S6 offers a faster and easier way to make immune cell typing more efficient.  With visible cell images and powerful data analysis

Surface Maker Analysis

Affinity of antibody
Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.The Countstar® Rigel S6 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

 Affinity of antibody

GFP Transfection Efficiency
In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar Rigel S6 enables users to get the result of a transfection efficiency assay quickly and accurately.

GFP Transfection Efficiency

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