Ihe atụ onyonyo nke yist Baker Saccharomyces cerevisiae
Onyonyo nke yist bred Saccharomyces cerevisiae enwetara ya na Countstar BioFerm. Ewepụtara ihe nlele site na usoro nrụpụta dị iche iche, nke nwere ntụpọ Methylene Blue (nke dị n'aka ekpe) yana Methylene Violet (n'aka nri nke ala)
Saccharomyces cerevisiae na ọkwa dị iche iche nke usoro ịgba ụka 2-nzọụkwụ
N'elu aka ekpe: Akụkụ nke onyonyo Countstar BioFerm na-egosi omenala mmalite, nke Methylene Blue (MB) metọsịrị.Ihe nlele ahụ nwere njupụta cell dị elu yana sel nwere ike dị ukwuu (onwu a tụrụ <5%).N'aka ekpe nke dị n'okpuru: Ihe nlele na-enweghị ntụpọ sitere na bioreactor a gbanyere ọhụrụ;buds na-ahụ anya nke ọma.N'aka nri ala: Ewere nlele na njedebe ikpeazụ nke usoro ịgba ụka, nke MB nwere ntụpọ 1:1 (onwu atụpụtara: 25%).Àkụ́ na-acha ọbara ọbara na-aka akara mkpụrụ ndụ nwụrụ anwụ, bụ́ ndị gbakwunyere ike ịcha agba MB, na-eduga na agba gbara ọchịchịrị nke mpịakọta cell dum.
Ntụnyere data nha
Eserese dị n'elu na-egosi ntunyere Countstar BioFerm na ngụ akwụkwọ ntuziaka, yana nnukwu ọdịiche dị ala na nsonaazụ nha, ma ọ bụrụ na atụnyere ọnụ ọgụgụ hemocytometer ntuziaka.
Ntụle akwụkwọ ntuziaka na akpaaka nkesa nkesa
Eserese ndị dị n'elu na-egosi oke nha nha dayameta Countstar BioFerm na nyocha akwụkwọ ntuziaka na hemocytometer.Dị ka n'akwụkwọ ntuziaka na-agụta ọnụ ọgụgụ sel dị ala 100 ka a na-enyocha, ụkpụrụ nkesa dayameta dị iche iche karịa na Countstar BioFerm, ebe nyochara ihe fọrọ nke nta ka ọ bụrụ 3,000 sel yist.
Mmụgharị nke ọnụ ọgụgụ cell na ọnụ ọgụgụ ọnwụ
25 aliquots nke diluted Saccharomyces cerevisiae Countstar BioFerm nyochara ihe nlele nke nwere ntinye aha nke 6.6 × 106 sel / mL n'otu aka ahụ yana n'ime hemocytometer ejiri aka.
Eserese abụọ a na-egosi mgbanwe dị elu na ọnụ ọgụgụ sel otu, nke ejiri aka mee na hemocytometer.N'ụzọ dị iche, Countstar BioFerm na-adịgasị iche naanị site na ọnụ ahịa aha na ntinye uche (aka ekpe) na ọnwụ (n'aka nri).
Saccharomyces cerevisiae na ọkwa dị iche iche nke usoro ịgba ụka 2-nzọụkwụ
Saccharomyces cerevisiae, Methylene Violet nwere ntụpọ wee jiri Countstar nyochaa ya Sistemụ BioFerm
Aka ekpe: Akụkụ nke onyonyo Countstar Bioferm enwetara Right: Otu ngalaba, sel ndị Countstar kpọrọ ya Algọridim ihe onyonyo BioFerm.Selụ nwere ike ime bụ okirikiri ndụ ndụ, mkpụrụ ndụ nwere ntụpọ (nwụrụ anwụ) gbara ya gburugburu akara na okirikiri odo (nke agbakwunyere maka broshuọ a nwere akụ edo edo).chịkọtara okirikiri pink gbara sel gburugburu.Ọnụ ọgụgụ dị elu nke mkpokọta mkpụrụ ndụ abụọ na-ahụ anya - ihe ngosi doro anya nke ọrụ mmalite nke omenala a, akụ na-acha odo odo, tinye aka na aka, akara mkpụrụ ndụ nwụrụ anwụ.
Ngụkọta histogram nke gbaa ụka yist na-eto ngwa ngwa na-edepụta ọkwa dị elu nke ọrụ ntolite, na-egosipụtakarị mkpokọta cell 2,
