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Quantitative Cell Cycle Analysis by Using Countstar FL Image Cytometer

 Introduction

Measuring the incorporation of DNA-binding dyes has been a well-established method for determining cellular DNA content in cell cycle analysis. Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. In cell division, cells containing increased amounts of DNA display proportionally increased fluorescence. Differences in fluorescence intensity are used to determine the DNA content in each phase of the cell cycle. The Countstar Rigel system (Fig.1) is a smart, intuitive, multifunctional cell analysis instrument that can obtain precise data in cell cycle analysis and can detect cytotoxicity by cell viability assay. The easy-to-use, automated procedure guides you to complete a cellular assay from imaging and data acquisition.

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