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GFP Transfection Efficiency Assay Using 293 and Propidium Iodide

Introduction

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms, it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism, or in selected organs or cells or interest. GFP can be introduced into animals or other species through transgenic techniques and maintained in their genome and that of their offspring.

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