Ezikhathini ze-COVID-19 ukuhlaziya kwamaseli egazi e-mononuclear cell (PBMCs) kanye namaphethini awo omaka we-CD kuyizilinganiso ezibalulekile eziletha idatha ebalulekile ukuze kuqondwe kangcono ukuqhubeka kokutheleleka nge-SARS-CoV-2 kubantu.
Ngokuvamile ukuhlaziywa kwe-PBMC kwamasampula egazi lonke kudla isikhathi
inqubo.I-Countstar Rigel ifinyeza lesi sikhathi sokuhlaziya ngokuphawulekayo ngokusebenzisa indlela yokwenza amabala ye-AO/PI.Isofthiwe yethuluzi inciphisa ukubala okujwayeleke ukwenza amaphutha kanye nezinyathelo zokuhlaziya okwengeziwe (ububanzi / izinga lokuhlanganisa).
I-Countstar Rigel iletha imiphumela enembayo neqhathanisekayo ngaphezu kwezithombe ezihlaziya kakhulu zamaseli e-CD4+ ngokushesha kunendlela evamile yokugeleza kwe-cytometry.Ngaphandle kwalokho, abahlaziyi be-Countstar Rigel sebevele bakufakazele ukunemba kwabo kanye nokuzala kabusha ezinqubweni eziningi zokukhiqiza ezilawulwa yi-cGMP zemithi yokugoma nezithako ezisebenzayo zemithi (ama-API) emhlabeni jikelele.
Buza uzakwenu wezentengiso wesifunda noma uxhumane nathi ngokuqondile ukuze uhlele idemo noma ukuhlolwa kwamamodeli we-Countstar Rigel.Ochwepheshe bethu bezinhlelo zokusebenza bakulungele ukukusiza esethulweni nasekuqeqesheni.
Umdwebo 1
Ingxenye yesithombe sensimu ekhanyayo, etholwe kusampula yegazi lonke le-PBMC yi-Countstar Rigel S3, iqukethe imfucumfucu eningi, amaplatelet nezinye izinto ezingachazwanga.
Umdwebo 2
Isithombe esiyimbondela, isigaba esifanayo, amaseli angcoliswe yi-AO/PI, Isiteshi 1 (Ex/Em 480nm / 535/40nm) Isiteshi 2 (Ex/Em: 525nm / 580/25nm : Okubomvu: iseli elifile, Okuhlaza: iseli elisebenzayo, Okuwolintshi: into engeyona ilebula, engacacisiwe
Umdwebo 3
Idatha ye-cytometry egelezayo uma iqhathaniswa nemiphumela ye-Countstar Rigel, ilinganisa ukulebula kwe-CD3-FITC kanye ne-CD4-PE yamaseli omzimba omzimba akhuthazwe yi-IL-6.
