Nyob rau lub sijhawm ntawm COVID-19 kev tshuaj xyuas ntawm peripheral ntshav mononuclear hlwb (PBMCs) thiab lawv cov qauv CD-kos cim yog qhov tseem ceeb ntsuas uas xa cov ntaub ntawv tseem ceeb kom nkag siab zoo dua txog kev kis tus kab mob los ntawm SARS-CoV-2 hauv tib neeg.
Feem ntau PBMC tsom xam ntawm tag nrho cov ntshav kuaj yog siv sijhawm
txheej txheem.Lub Countstar Rigel txo lub sij hawm tsom xam no kom luv los ntawm kev siv AO/PI staining method.Cov cuab yeej software txo qis kev suav yuam kev thiab cov kauj ruam hauv kev tshuaj xyuas ntxiv (inch / aggregation rate).
Lub Countstar Rigel muab cov txiaj ntsig zoo thiab sib piv ntxiv rau cov duab siab ntawm CD4+ hlwb sai dua li cov qauv cytometry mus kom ze.Ntxiv rau qhov ntawd, Countstar Rigel cov kws tshuaj ntsuam xyuas twb tau ua pov thawj lawv qhov tseeb thiab kev tsim tawm hauv ntau cGMP cov txheej txheem tsim khoom rau cov tshuaj tiv thaiv thiab cov khoom xyaw tshuaj nquag (APIs) thoob ntiaj teb.
Nug koj tus khub muag khoom hauv cheeb tsam lossis tiv tauj peb ncaj qha los teem sijhawm ua qauv lossis kev ntsuam xyuas ntawm Countstar Rigel qauv.Peb cov kws tshaj lij daim ntawv thov tau npaj los pab koj hauv kev taw qhia thiab kev cob qhia.
Fig. 1
Ntu ntawm daim duab ci ci, tau los ntawm tag nrho cov ntshav PBMC qauv los ntawm Countstar Rigel S3, muaj ntau cov khib nyiab, platelets thiab lwm yam khoom tsis tau txhais
Daim duab 2
Overlay duab, tib seem, hlwb stained los ntawm AO/PI, Channel 1 (Ex/Em 480nm / 535/40nm) Channel 2 (Ex/Em: 525nm / 580/25nm: Liab: cell tuag, ntsuab: siv tau cell, Txiv kab ntxwv: non-labeled, unspecific object
Daim duab 3
Flow cytometry cov ntaub ntawv piv rau Countstar Rigel cov txiaj ntsig, suav nrog CD3-FITC thiab CD4-PE daim ntawv lo ntawm lub cev tiv thaiv kab mob los ntawm IL-6
